Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Tobermolite, Perlite and Polyurethane Packing Materials on Methanotrophic Activity

메탄산화세균의 활성에 미치는 tobermolite, perlite 및 Polyurethane 담체의 영향

  • Jeong, So-Yeon (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Yoon, Hee-Young (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Kim, Tae Gwan (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
  • 정소연 (이화여자대학교 환경공학과) ;
  • 윤희영 (이화여자대학교 환경공학과) ;
  • 김태관 (이화여자대학교 환경공학과) ;
  • 조경숙 (이화여자대학교 환경공학과)
  • Received : 2013.01.09
  • Accepted : 2013.03.31
  • Published : 2013.06.28
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Abstract

Biofilters for the removal of methane using tobermolite, perlite and polyurethane as packing materials have been undergoing recent development. The effects of these packing materials on methane oxidation activity were evaluated in this study. Mixed methanotrophs (consortia A, B, C and D) from wetland and landfill soils were used as the inoculum sources. The influences of packing materials, consisting of tobermolite, perlite, and polyurethane, on the methane oxidation rate and methanotrophic bio-mass, were estimated. When perlite was added into the methanotrophic cultures, the methane oxidation rate was more than twice that of the control (without packing materials), and the methanotrophic biomass increased more than 10 fold. The ratio of methanotrophic bacteria to total bacteria under with tobermolite packing material was higher than the control and the other packing materials, indicating that tobermolite can serve as a specific packing material where dominance of methanotrophs is desired. Therefore, perlite and tobermolite provide habitats which increase the activity of methanotrophic bacteria, and these packing materials are promising for use in methane oxidation processes.

Tobermolite, perlite 및 polyurethane을 충전재로 이용한 바이오필터에 의한 메탄제거 기술이 개발되고 있다. 본 연구에서는 이들 충전재가 메탄산화능에 미치는 영향을 알아보았다. 습지토양과 매립지토양에서 분리한 혼합 메탄산화세균(consortium A, B, C and D)를 접종원으로 하고, 메탄산화속도와 메탄산화세균 수에 미치는 담체(perlite, tobermolite,polyurethane)의 영향을 조사하였다. Perlite를 첨가한 경우 메탄산화속도는 대조군 (담체 미첨가)보다 두 배 이상 증가하였고, 메탄산화세균 수도 10배 이상 증가하였다. Tobermolite를 첨가한 경우에는 일반세균 수 대비 메탄산화세균의 비율이 대조군과 다른 담체에 비해 높았다. 이는 tobermolite가 메탄산화세균이 우점할 수 있는 특이적 담체로 작용함을 시사한다. 이상의 결과로 부터 perlite와 tobermolite는 메탄산화세균의 활성을 증가시키는 서식지를 제공하며 메탄산화 공정시스템에 적용 시 좋은 담체의 역할을 할 수 있을 것으로 기대된다.

Keywords

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