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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimization of Cellulolytic Enzyme Production for newly isolated Bacillus sp. H9-1 from Herbivore Feces

초식동물 배설물로부터 분리한 Bacillus sp. H9-1의 섬유소 분해효소생산 최적화

  • Yoon, Young Mi (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • An, Gi Hong (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Jung Kon (Animal Environment Division, National Institute of Animal Science, RDA) ;
  • Cha, Young-Lok (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Park, Yu Ri (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Ahn, Jong-Woong (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Moon, Youn-Ho (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Ahn, Seung-Hyun (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Koo, Bon-Cheol (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Park, Kwang-Geun (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
  • 윤영미 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 안기홍 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 김중곤 (농촌진흥청 국립축산과학원 축산환경과) ;
  • 차영록 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 박유리 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 안종웅 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 문윤호 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 안승현 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 구본철 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 박광근 (농촌진흥청 국립식량과학원 바이오에너지작물센터)
  • Received : 2012.11.28
  • Accepted : 2013.01.15
  • Published : 2013.02.27
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Abstract

This study was performed to find cellulolytic strain of enzymatic saccharification for bioethanol production. Cellulolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-Do. The celluloytic strain was selected by congo red staining and DNS method. Among the isolated strains, H9-1 strain isolated from the feces of rabbit has the highest CMCase activity. H9-1 strain was identified as Bacillus sp. based on 16S rDNA gene sequencing. The optimal conditions for CMCase activity by Bacillus sp. H9-1 were at $40^{\circ}C$ and at initial pH 8.

Keywords

References

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