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http://dx.doi.org/10.7841/ksbbj.2013.28.1.42

Optimization of Cellulolytic Enzyme Production for newly isolated Bacillus sp. H9-1 from Herbivore Feces  

Yoon, Young Mi (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
An, Gi Hong (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Kim, Jung Kon (Animal Environment Division, National Institute of Animal Science, RDA)
Cha, Young-Lok (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Park, Yu Ri (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Ahn, Jong-Woong (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Moon, Youn-Ho (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Ahn, Seung-Hyun (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Koo, Bon-Cheol (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Park, Kwang-Geun (Bioenergy Crop Research Center, National Institute of Crop Science, RDA)
Publication Information
KSBB Journal / v.28, no.1, 2013 , pp. 42-47 More about this Journal
Abstract
This study was performed to find cellulolytic strain of enzymatic saccharification for bioethanol production. Cellulolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-Do. The celluloytic strain was selected by congo red staining and DNS method. Among the isolated strains, H9-1 strain isolated from the feces of rabbit has the highest CMCase activity. H9-1 strain was identified as Bacillus sp. based on 16S rDNA gene sequencing. The optimal conditions for CMCase activity by Bacillus sp. H9-1 were at $40^{\circ}C$ and at initial pH 8.
Keywords
bioethanol; herbivore feces; Bacillus sp. H9-1; CMCase activity;
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Times Cited By KSCI : 5  (Citation Analysis)
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