KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Pretreatment of Wastepaper using Aqueous Glycerol to enhance Enzymatic Hydrolysis

효소 가수분해 향상을 위해 glycerol 수용액을 사용한 폐지의 전처리

  • Seo, Dong Il (Department of Chemical & Biological Engineering, Gyeongsang National University) ;
  • Kim, Chang-Joon (Department of Chemical & Biological Engineering, Gyeongsang National University) ;
  • Kim, Sung Bae (Department of Chemical & Biological Engineering, Gyeongsang National University)
  • 서동일 (경상대학교 공과대학 생명화학공학과) ;
  • 김창준 (경상대학교 공과대학 생명화학공학과) ;
  • 김성배 (경상대학교 공과대학 생명화학공학과)
  • Received : 2013.01.23
  • Accepted : 2013.02.22
  • Published : 2013.02.27
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Abstract

Pretreatment of wastepaper using aqueous glycerol was investigated to enhance the enzymatic hydrolysis. The effects of four factors (solid/liquid ratio, glycerol concentration, acid concentration, and reaction time) on the dissolution yield, the removal of cellulose, hemicellulose and lignin, and the enzymatic digestibility were examined at $150^{\circ}C$. The 1/8 of solid/liquid was determined to perform the reaction uniformly, and the 93% of glycerol concentration was found to be a minimum concentration to conduct the reaction under atmospheric pressure. Also, it was found that the acid concentration and reaction time were strongly related to the dissolution yield and the removal of cellulose, hemicellulose and lignin, but moderately to the enzymatic digestibility. At an optimum condition of $150^{\circ}C$, 1 h and 1% acid concentration, 56% and 49% of hemicellulose and lignin, respectively, were removed, while only 4% of cellulose was removed. The enzymatic digestibility at this condition was 86%, meaning that 83% of the glucan present in the initial substrate was converted to glucose. Compared to glycerol with ethylene glycol as a pretreatment solvent, glycerol is much cheaper than ethylene glycol, but ethylene glycol is superior to glycerol in delignification.

Keywords

References

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