한국가시화정보학회지 (Journal of the Korean Society of Visualization)

  • 제11권2호
  • /
  • Pages.41-45
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  • 2013
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  • 1598-8430(pISSN)
  • /
  • 2093-808X(eISSN)
한국가시화정보학회 (The Korean Society of Visualization)
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고속 트립토판 자가형광 이미징을 위한 시공간적 집중 기반의 라인 스캐닝 이광자 현미경 개발

Development of line-scanning two-photon microscopy based on spatial and temporal focusing for tryptophan based auto fluorescence imaging

  • 이준호 (포항공과대학교 기계공학과) ;
  • 남효석 (포항공과대학교 기계공학과) ;
  • 김기현 (포항공과대학교 기계공학과)
  • 투고 : 2013.09.05
  • 심사 : 2013.09.26
  • 발행 : 2013.09.30
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초록

Two-photon microscopy (TPM) is minimally-invasive 3D fluorescence microscopy based on nonlinear excitation, and TPM can visualize cellular structures based on auto-fluorescence. Line-scanning TPM is one of high-speed TPM methods without sacrificing the image resolution by using spatial and temporal focusing. In this paper, we developed line-scanning TPM based on spatial and temporal focusing for auto-fluorescence imaging by exciting the tryptophan. Laser source for this system was an optical parametric oscillator (OPO) and it made near 570 nm femtosecond pulse laser. It had 200fs pulse width and 1.72 nm bandwidth, so that the achievable depth resolution was 2.41um and field of view (FOV) is 10.8um. From the characterization, our system has 3.0 um depth resolution and 12.3 um FOV. We visualized fixed leukocyte cell sample and compared with point scanning system.

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참고문헌

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