• IMMUNE NETWORK
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• v.9 no.5
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• pp.153-157
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• 2009

The leukocyte adhesion to endothelium is pivotal in leukocyte recruitment which takes place during inflammatory, autoimmune and infectious conditions. The interaction between leukocytes and endothelium requires an array of adhesion molecules expressed on leukocytes and endothelial cells, thereby promoting leukocyte recruitment into sites of inflammation and tissue injury. Intervention with the adhesion molecules provides a platform for development of anti-inflammatory therapeutics. This review will focus on developmental endothelial locus-1 (Del-1), an endogenous inhibitor of leukocyte adhesion.

• Journal of Korean Neurosurgical Society
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• v.38 no.3
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• pp.221-226
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• 2005

Objective : Reactive oxygen metabolites and polymorphonuclear leukocytes have been implicated in the pathophysiology of reperfusion injury. The mechanisms involved in superoxide-mediated leukocyte adherence remain unclear, however, nitric oxide[NO] may contribute to this response. The present study is undertaken to elucidate mechamisms controlling NO based mechanisms that regulated leukocyte-endothelial interactions in the cerebral vasculature after global cerebral ischemia and reperfusion. Methods : Pial venular leukocyte adherence of anesthetized newborn piglets was quantified by in situ fluorescence videomicroscopy through closed cranial windows during basal conditions and during 2hours of reperfusion after global ischemia induced by 9minutes of asphyxia. Nitric oxide synthase[NOS] was inhibited by local window superfusion of L-nitroarginine[NA]; superfusion of sodium nitroprusside[SNP] was used to donate NO. Results : The mean number of adherent leukocytes to cerebral venules in the 9minutes asphyxia and 2hours reperfusion group were $161{\pm}19$ compared with $13{\pm}4$ in the nonasphyxial group. Superfusion of L-NA through the cranial window for 2hours resulted in leukocyte adherence similar to that observed during the initial 2hours of reperfusion after asphyxia. Leukocyte adherence was not additionally increased in asphyxic animal treated with L-NA. SNP inhibited asphyxia induced leukocyte adherence back to control levels. Conclusions : Nitric oxide inhibits leukocyte adherence to cerebral venules during the initial hours of reperfusion after asphyxia, and that NO supplementation inhibit asphyxia induced leukocyte adherence back to control levels. These results indicate that NO is an important factor in ischemia-reperfusion induced leukocyte adherence.

• Biomedical Science Letters
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• v.7 no.2
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• pp.91-98
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• 2001

The present study was prospectively designed to assess the clinical effect of leukocyte-depleted blood cardioplegic solution (BCS) on myocardium during cardiac surgery with cardiopulmonary bypass (CPB). 30 adult patients scheduled for elective cardiac surgery were divided into control group (n＝15), which infused routine BCS, and leukocyte-depleted (LD) group (n＝15), which infused leukocyte-depleted BCS. Total and differential leukocyte counts in BCS, malondialdehyde (MDA) and troponin-T (TnT) concentrations in coronary sinus blood, and cardiac index (CI) were measured at preoperative and postoperative period. The BCS in LD group had less total leukocyte counts with neutropenia than that in control group (P＜0.01). MDA (3.70$\pm$0.35 vs 5.90$\pm$0.57 $\mu$mol/L, p<0.05) and TnT (0.42$\pm$0.03 vs 0.60$\pm$0.09 ng/mL, p<0.05) were significantly low in LD group compared with control group, while LD group had higher CI (3.28$\pm$0.16 L/min/$m^2$, p<0.05) than control group (2.69$\pm$0.18 L/min/$m^2$). These results suggest that leukocyte-depleted blood cardioplegic solution has a better myocardial protective effect with less generations of oxygen free radicals and ischemia/reperfusion injury.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.4
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• pp.586-590
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• 2009

Leukocyte adhesion deficiency is rare inherited defect on phagocytic function resulting lack of leukocyte cell surface expression of $\beta2$ integrin molecule that are essential for leukocyte adhesion to endothelial cells and chemotaxis. Clinical features of patients with leukocyte adhesion deficiency type I include recurrent necrotic infection of the skin mucous membranes, and intestinal tract with septicemia, and omphalitis arising from delayed umbilical cord separation. Oral manifestations are severe progressive periodontitis with alveolar bone loss, periodontal pockets, and partial and total premature loss of the deciduous and permanent dentitions. We report a case of leukocyte adhesion deficiency type I in a 5-year-old child with severe periodontitis. In order to prevent local and systemic infection, we controlled periodontal disease with periodic oral prophylaxis. Oral swabs and blood cultures were perfomed for suspected infection, so that optimal measures were taken through the use of appropriate antibiotics.

• Neonatal Medicine
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• v.25 no.4
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• pp.191-195
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• 2018

Leukocyte adhesion deficiency is a rare primary immunodeficiency and autosomal recessive disorder caused by a mutation in the gene encoding CD18, which is a constituent of leukocyte integrins. Clinical features usually begin with a delay in the separation of the umbilical cord in the neonatal period, and are characterized by marked leukocytosis with infection, delayed wound healing, and repeated bacterial and fungal infections. We experienced a case of leukocyte adhesion deficiency diagnosed in the neonatal period, in which a late preterm infant admitted to neonatal intensive care unit presented with a septic hip. Flow cytometry analysis of whole blood showed a decrease in the expression of CD11b/CD18. This is the first case of leukocyte adhesion deficiency with neonatal septic hip diagnosed in Korea.

• Korean Journal of Veterinary Research
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• v.36 no.2
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• pp.337-348
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• 1996

The proportion of leukocyte subpopulation in the host is of a great importance in understanding their functions and disease progress. Many methods have been developed to seperate leukocytes and to measure their activities. Characterization of immune cell subpopulations in Korean native cattle was performed using a set of monoclonal antibodies specific which are specific to bovine leukocyte differentiation antigen. Peripheral blood leukocytes from fifty Korean native and ten Holstein cattle were collected and analyzed for the investigation of leukocyte subpopulation by using monoclonal antibodies and flow cytometry. The result indicated that Korean native cattle have significantly higher proportion of leukocyte subpopulations expressing MHC class II molecules and BoCD4 than Holstein cattle.

• Journal of the Institute of Convergence Signal Processing
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• v.15 no.4
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• pp.128-134
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• 2014

In this paper, we propose a detection method of the leukocyte motions in a microvessel by using spatiotemporal image analysis. The leukocyte motions that adhere to blood vessel walls can be visualized to move along the blood vessel wall's contours in a sequence of images. In this proposal method, we use the constraint that the leukocytes move along the blood vessel wall's contours and detect the leukocyte motions by using the spatiotemporal image analysis method. The generated spatiotemporal image is processed by a special-purpose orientation-selective filter and then subsequent grouping processes are done. The subsequent grouping processes select and group the leukocyte trace segments among all the segments obtained by simple thresholding and skeletonizing operations. Experimental results show that the proposed method can stably detect the leukocyte motions even when multiple leukocyte traces intersect each other.

• The Journal of the Korean dental association
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• v.21 no.10 s.173
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• pp.805-812
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• 1983

For the assessment of peripheral blood leukocyte chemotaxis dysfunction in rapidly progressive periodontitis, peripheral blood leukocyte of 13 patients were isolated, and tested by the method of blind well chamber technique which was recently developed by Park. Data analysis was performed from the speed of cell migration from the bottom of filter paper to the top for 18 minutes at the condition of chemo-attractant(Zymosan-activated serum). Eight of the 13 patients (62%) exhibited statistically significant inhibition of leukocyte chemotaxis, 4 patients revealed normal function and the other one patients showed elevated leukocyte chemotaxis. Four of the 8 patients in chemotaxis dysfunction showed significant inhibition of random migration and chemotaxis dysfunction both. Two of these 4 patients exhibited random migration impairment, elevation of C.D.I. and chemotaxis dysfunction. The results indicated that the progression of rapidly progressive periodontitis was influenced by the leukocyte chemotaxis dysfunction, and half of these dysfunction was associated with cell-defect of leukocyte.

• Bulletin of the Korean Chemical Society
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• v.35 no.12
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• pp.3489-3494
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• 2014

In search of new antileukopenia agents, twenty dithiolopyrrolone derivatives were synthesized and evaluated for their leukocyte-increasing activities in normal mice. Among the synthesized compounds 4-23, compounds 5 and 6 showed significant leukocyte-increasing activity ( p < 0.01), and compounds 4, 9 and 16 had a moderate effect ( p < 0.05). Compound 5 also displayed stronger leukocyte-increasing activity than that of the positive recombinant human granulocyte colony stimulating factor (rhG-CSF). Above all, compound 5 would be a potential antileukopenia agent which deserved further research.

• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.465-470
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• 1994

This study was undertaken to measure the activity of myeloperoxidase and leukocyte peroxidase of hen. 70 hens were decapitated to observe the activity of enzymes according to ages. The activity of peroxidase by the Lowry's method with bovine serum albumin as standard. Gel filtration chromatography was carried out of sephacryl S-300 column. The results obtained were summarized as follows; 1. The mean of specific activity of myeloperoxidase and leukocyte peroxidase was 16.80(units/mg) and 15(units/mg), respectively. 2. The specific activity of myeloperoxidase in 35 days hen was significantly increased and showed almost the same level of activity to 350 days hen. 3. The specific activity of leukocyte peroxidase in 35 days hen was significantly increased and showed a little increased tendency from 210 days to 350 days hen. 4. On the sephacryl S-300 column chromatography, two separated peaks of myeloperoxidase activity were observed. The molecular weights of myeloperoxidase were 57,000 dalton and 13,700 dalton.