The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of OMC-2010 extracts on cytokine productions in mouse spleen cells

OMC-2010 추출물이 마우스의 비장세포 cytokine 생성에 미치는 영향

  • Bae, Gi-Sang (Hanbang Body-fluid Research Center, Wonkwang University) ;
  • Park, Kyoung-Chel (Dept. of Herbology, College of oriental Medicine, Wonkwang University) ;
  • Choi, Sun-Bok (Dept. of Herbology, College of oriental Medicine, Wonkwang University) ;
  • Jo, Il-Joo (Dept. of Herbology, College of oriental Medicine, Wonkwang University) ;
  • Seo, Sang-Wan (ChungBuk Oriental Medicine Center, ChungBuk Techno Park) ;
  • Kim, Jong-Jin (ChungBuk Oriental Medicine Center, ChungBuk Techno Park) ;
  • Shin, Yong-Kook (ChungBuk Oriental Medicine Center, ChungBuk Techno Park) ;
  • Kim, Min-Sun (Division of Mass Spectrometry Research, Korea Basic Science Institute) ;
  • Park, Kyu-Hwan (Division of Mass Spectrometry Research, Korea Basic Science Institute) ;
  • Kim, Hyun-Sik (Division of Mass Spectrometry Research, Korea Basic Science Institute) ;
  • Song, Ho-Joon (Dept. of Herbology, College of oriental Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Hanbang Body-fluid Research Center, Wonkwang University)
  • 배기상 (원광대학교 한방체액조절연구센터) ;
  • 박경철 (원광대학교 한의과대학 본초학교실) ;
  • 최선복 (원광대학교 한의과대학 본초학교실) ;
  • 조일주 (원광대학교 한의과대학 본초학교실) ;
  • 서상완 (충북테크노파크 전통의약산업센터) ;
  • 김종진 (충북테크노파크 전통의약산업센터) ;
  • 신용국 (충북테크노파크 전통의약산업센터) ;
  • 김민선 (한국기초과학지원연구원 질량분석연구부) ;
  • 박규환 (한국기초과학지원연구원 질량분석연구부) ;
  • 김현식 (한국기초과학지원연구원 질량분석연구부) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 박성주 (원광대학교 한방체액조절연구센터)
  • Received : 2012.08.20
  • Accepted : 2012.08.31
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study was performed to estimate the effects of OMC-2010 extract on cytokine production in mouse spleen cells. Methods : Mouse spleen cells were pre-treated with ethanol and water extract of OMC-2010 for 1 h, then stimulated with lipopolysaccharide (LPS, 1 ${\mu}g/ml$) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokines. Results : OMC-2010 ethanol extract significantly inhibited the LPS-induced interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and IL-5 mRNA expressions, but not shown such changes in IL-6, IL-4, IL-13. OMC-2010 water extract significantly inhibited the LPS-induced TNF-alpha, and IL-5 mRNA expressions, but not shown such changes in IL-1beta, IL-6, IL-4, IL-13. Conclusions : Theses results could suggest that both ethanol and water OMC-2010 extract could inhibit the TNF-alpha and IL-5 mRNA expression.

Keywords

References

  1. Germano G, Mantovani A, Allavena P. Targeting of the innate immunity/inflammation as complementary anti-tumor therapies. Ann Med. 2011 ; 43(8) : 581-93. https://doi.org/10.3109/07853890.2011.595732
  2. Khayyamian S, Hutloff A, Buchner K, Grafe M, Henn V, Kroczek RA, Mages HW. ICOS-ligand, expressed on human endothelial cells, costimulates Th1 and Th2 cytokine secretion by memory CD4+ T cells. Proc Natl Acad Sci U S A. 2002 ; 99(9) : 6198-203. https://doi.org/10.1073/pnas.092576699
  3. Marzo AL, Vezys V, Williams K, Tough DF, Lefrancois L. Tissue-level regulation of Th1 and Th2 primary and memory CD4 T cells in response to Listeria infection. J Immunol. 2002 ; 168(9) : 4504-10. https://doi.org/10.4049/jimmunol.168.9.4504
  4. Jo JK, An CG, Kim KJ, Kim NK, Park MC. Effects of Kumsooyukkun-jeon extract on the Ovalbumin-Induced Allergic Asthma in Mice. JKOOD. 2007 ; 20(3) : 107-17.
  5. Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci. U.S.A. 1975 ; 72 (9) : 3666-70. https://doi.org/10.1073/pnas.72.9.3666
  6. Beutler B, Greenwald D, Hulmes JD, Chang M, Pan YC, Mathison J, Ulevitch R, Cerami A. Identity of tumour necrosis factor and the macrophage-secreted factor cachectin. Nature 1985 ; 316 (6028) : 552-4. https://doi.org/10.1038/316552a0
  7. Micheau O, Tschopp J. Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes. Cell 2003 ; 114 (2) : 181-90. https://doi.org/10.1016/S0092-8674(03)00521-X
  8. Mikocka-Walus AA, Turnbull DA, Moulding NT, Wilson IG, Andrews JM, Holtmann GJ. Controversies surrounding the comorbidity of depression and anxiety in inflammatory bowel disease patients: a literature review. Inflammatory Bowel Diseases 2007 ; 13 (2) : 225-234. https://doi.org/10.1002/ibd.20062
  9. Tracey KJ, Fong Y, Hesse DG, Manogue KR, Lee AT, Kuo GC, Lowry SF, Cerami A. Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia. Nature 1987 ; 330 (6149) : 662-64. https://doi.org/10.1038/330662a0
  10. Dubucquoi S, Desreumaux P, Janin A, Klein O, Goldman M, Tavernier J, Capron A, Capron M. Interleukin 5 synthesis by eosinophils: association with granules and immunoglobulin-dependent secretion. J Exp Med. 1994 ; 179 (2) : 703-8. https://doi.org/10.1084/jem.179.2.703
  11. Sanderson CJ. Interleukin-5, eosinophils, and disease. Blood 1992 ; 79 (12) : 3101-9.
  12. Bradding P, Roberts JA, Britten KM, Montefort S, Djukanovic R, Mueller R, Heusser CH, Howarth PH, Holgate ST. Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines. Am J Respir Cell Mol Biol. 1994 ; 10 (5) : 471-80. https://doi.org/10.1165/ajrcmb.10.5.8179909
  13. Shen HH, Ochkur SI, McGarry MP, Crosby JR, Hines EM, Borchers MT, Wang H, Biechelle TL, O'Neill KR, Ansay TL, Colbert DC, Cormier SA, Justice JP, Lee NA, Lee JJ. A causative relationship exists between eosinophils and the development of allergic pulmonary pathologies in the mouse. J Immunol. 2003 ; 170 (6) : 3296-305. akchulpansa. 1996 : 170-1. https://doi.org/10.4049/jimmunol.170.6.3296

Cited by

  1. OMC-2010 구성약재가 마우스의 비장세포 cytokine 생성에 미치는 영향 vol.27, pp.6, 2012, https://doi.org/10.6116/kjh.2012.27.6.49
  2. OMC-2010 구성약재 배합추출물 투여가 Ovalbumin으로 유도한 마우스 알레르기성 기관지 천식에 미치는 영향 vol.28, pp.5, 2012, https://doi.org/10.6116/kjh.2013.28.5.87