The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Antioxidant Activity of Nelumbinis Stamen in GC-2 spd(ts) Cells

연수(蓮鬚)가 GC-2 spd(ts) Cell의 항산화에 미치는 영향

  • Park, Eun-Hwa (Dept. of Herbal Pharmaceutical Science, Joongbu University) ;
  • Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kil, Ki-Jeong (Dept. of Herbal Pharmaceutical Science, Joongbu University) ;
  • Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 박은화 (중부대학교 한방제약과학과) ;
  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 길기정 (중부대학교 한방제약과학과) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Received : 2012.08.01
  • Accepted : 2012.09.10
  • Published : 2012.09.30
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Abstract

Objectives : The purpose of this study was to estimate the antioxidant activity of water extract of Nelumbinis stamen (WNS) in GC-2 spd (ts) cells. Methods : we investigated the effect of WNS in mouse GC-2 spd (ts) cells by MTT assay. The protective effects of WNS against hydrogen peroxide-induced oxidative stress in GC-2 spd (ts) cells were examined by measuring cell viability. Lipid peroxidation levels and catalase were measured. Results : WNS showed cell viability as 101.9, 108.9, 111.8, 125.8, 134.5% in 2.5, 5, 10, 25, 50 ${\mu}g/ml$ concentrations, respectively. The protective effect of WNS concentration was 2.5 ${\mu}g/ml$ against hydrogen peroxide-induced oxidative stress in GC-2 spd (ts) cells. LPO were decreased significantly at 2.5, 5, 25 ${\mu}g/ml$ of WNS concentrations. Catalase activity was significantly increased at 2.5, 5 and 10 ${\mu}g/ml$ of WNS concentrations, respectively. Conclusions : In conclusion, WNS has antioxidant activities in GC-2 spd(ts) cells against oxidative stress.

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References

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