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http://dx.doi.org/10.6116/kjh.2012.27.5.21

Effects of OMC-2010 extracts on cytokine productions in mouse spleen cells  

Bae, Gi-Sang (Hanbang Body-fluid Research Center, Wonkwang University)
Park, Kyoung-Chel (Dept. of Herbology, College of oriental Medicine, Wonkwang University)
Choi, Sun-Bok (Dept. of Herbology, College of oriental Medicine, Wonkwang University)
Jo, Il-Joo (Dept. of Herbology, College of oriental Medicine, Wonkwang University)
Seo, Sang-Wan (ChungBuk Oriental Medicine Center, ChungBuk Techno Park)
Kim, Jong-Jin (ChungBuk Oriental Medicine Center, ChungBuk Techno Park)
Shin, Yong-Kook (ChungBuk Oriental Medicine Center, ChungBuk Techno Park)
Kim, Min-Sun (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Park, Kyu-Hwan (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Kim, Hyun-Sik (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Song, Ho-Joon (Dept. of Herbology, College of oriental Medicine, Wonkwang University)
Park, Sung-Joo (Hanbang Body-fluid Research Center, Wonkwang University)
Publication Information
The Korea Journal of Herbology / v.27, no.5, 2012 , pp. 21-25 More about this Journal
Abstract
Objective : This study was performed to estimate the effects of OMC-2010 extract on cytokine production in mouse spleen cells. Methods : Mouse spleen cells were pre-treated with ethanol and water extract of OMC-2010 for 1 h, then stimulated with lipopolysaccharide (LPS, 1 ${\mu}g/ml$) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokines. Results : OMC-2010 ethanol extract significantly inhibited the LPS-induced interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and IL-5 mRNA expressions, but not shown such changes in IL-6, IL-4, IL-13. OMC-2010 water extract significantly inhibited the LPS-induced TNF-alpha, and IL-5 mRNA expressions, but not shown such changes in IL-1beta, IL-6, IL-4, IL-13. Conclusions : Theses results could suggest that both ethanol and water OMC-2010 extract could inhibit the TNF-alpha and IL-5 mRNA expression.
Keywords
OMC-2010; lipopolysaccharide (LPS); splenocyte; inflammation;
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