Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Evaluation of a Rapid Immunochromatography Technique for Determination of Cd in Soils and Rice Grains

면역크로마토그래피를 이용한 토양 및 쌀의 Cd 간이진단법 평가

  • Lee, Sang-Phil (Department of Biological Environment, Kangwon National University) ;
  • Kim, Rog-Young (Department of Biological Environment, Kangwon National University) ;
  • Abe, Kaoru (National Institute for Agro-Environmental Sciences) ;
  • Kim, Sung-Chul (Department of Bio Environmental Chemistry, Chungnam Natinal University) ;
  • Kim, Won-Il (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Yang, Jae E. (Department of Biological Environment, Kangwon National University)
  • 이상필 (강원대학교 바이오자원환경학과) ;
  • 김록영 (강원대학교 바이오자원환경학과) ;
  • ;
  • 김성철 (충남대학교 생물환경화학과) ;
  • 김원일 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 양재의 (강원대학교 바이오자원환경학과)
  • Received : 2011.11.20
  • Accepted : 2012.12.07
  • Published : 2012.12.31
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Abstract

Cadmium is known to be very toxic to human health and can be relative easily translocated from soils in plants. Therefore, a rapid method for screening Cd in soils and crops has become more and more important. For this reason, we examined a rapid immunochromatograpy (ICG) test kit which uses antigen-antibody reaction based on immunoassay and chromatography. Soils and rice grains collected from mine waste-contaminated sites were determined for their Cd contents using this kit. For comparison purposes, 0.1 M HCl and ICP-OES were employed as a conventional extraction and determination method. Cadmium contents in rice grains determined using ICG technique were $0.46{\sim}2.39mg\;kg^{-1}$ and Cd contents determined using 0.1 M HCl and ICP-OES were $0.52{\sim}1.97mg\;kg^{-1}$. The correlation between these two Cd contents were statistically significant ($r^2$=0.930). The results of Cd contents in soils also showed a statistically significant relationship between these two methods ($r^2$=0.975). On the basis of these results, ICG technique can be applied to rapidly quantify Cd in crops and soils. However, further research is necessary to apply ICG technique for the field screening.

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References

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