• Bulletin of the Korean Chemical Society
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• 제26권2호
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• pp.268-272
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• 2005

Different detection characteristics of fluorescence immunochromatography method and high performance liquid chromatography (HPLC) method for the analysis of cyanobacterial toxins were studied. In particular, low and high limits of detection, detection time and reproducibility and detectable microcystin species were compared when fluorescence immunochromatography method and high performance liquid chromatography method were applied for the detection of microcystin (MC), a cyclic peptide toxin of the freshwater cyanobacterium Microcystis aeruginosa. A Fluorescence immunochromatography assay system has the unique advantages of short detection time and low detection limit, and high performance liquid chromatography detection method has the strong advantage of individual quantifications of several species of microcystins.

• ALGAE
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• 제28권3호
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• pp.289-296
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• 2013

For the quantitative detection of algal toxin, microcystin, a chemiluminescence immunochromatographic assay method was developed. The developed system consists of four parts, chemiluminescence assay strip (nitrocellulose membrane), horse radish peroxidase labeled microcystin monoclonal antibodies, chemiluminescence substrate (luminol and hydrogen peroxide), and luminometer. The performance of the chemiluminescence immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. The detection limit of chemiluminescence immunochromatographic assay system is several orders of magnitude lower than with HPLC. The chemiluminescence immunochromatography and HPLC results correlated very well with the correlation coefficient ($r^2$) of 0.979.

• 한국수산과학회지
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• 제48권4호
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• pp.459-465
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• 2015

We tested biomarker systems [enzyme-linked immunosorbent assay (ELISA) and immunochromatography assay (ICG) kits] for the screening of endocrine-disrupting chemicals in contaminated environments using antibodies resulting from $17{\beta}$-Estradiol-induced vitellogenin (Vtg) in the wild scorpion fish Sebastiscus marmoratus. Monoclonal antibodies of two clones (S28 and S15) were used as capture and tracer antibodies for ELISA and ICG assays. ELISA detected Vtg at levels greater than $0.1{\mu}g/mL$, while ICG detected Vtg at levels greater than $1{\mu}g/mL$. However, the ICG system was able to detect antibodies from $17{\beta}$-Estradiol-induced Vtg serum that had been diluted 1,000 times. Our results suggest that previously developed biomarker assays can be used as detection systems to detect known endocrine-disrupting chemicals in contaminated environments, and to measure their activity.

• Bulletin of the Korean Chemical Society
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• 제32권1호
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• pp.149-152
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• 2011

A new chemiluminescence immunochromatographic analysis system with high sensitivity and high reproducibility was developed for the determination of microcystins (MCs) in water. Horse radish peroxidase (HRP) labeled microcystin monoclonal antibody was used for the sensitive chemiluminescence detection. The chemiluminescence immunochromatographic analysis system was composed of microcystin LR (MCLR)-monoclonal antibody (mAb)-Horse Radish Peroxidase (HRP) conjugate, MCLR-BSA conjugate, luminol, hydrogen peroxide mixture solution, an immunochromatographic assay strip and luminometer. To detect the concentration of microcystins in water, we utilized one spot analysis of the strip instead of flow type analysis. We could detect the microcystins in water at a concentration as low as 9.45 pg/mL with the chemiluminescence (CL) detection.

• 한국산학기술학회논문지
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• 제14권7호
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• pp.3419-3424
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• 2013

B형간염표면 항원 및 항체(HBsAg와 anti-HBs)에 대한 검사 방법으로 화학발광면역검사법(CIA)을 포함한 면역검사법을 주로 사용하고 있으나 최근 간편한 면역크로마토그래피법(ICA)의 사용이 늘고 있는 추세이다. 본 연구에서는 CIA법을 이용한 정량검사법과 비교하여 신속검사인 ICA법의 민감도를 평가하고자 하였다. 대학병원에 간염검사를 의뢰한 검체 중 CIA법에서 양성 및 음성으로 나온 120 검체를 선정하여 ICA법으로 분석하였다. ICA법과 CIA법의 일치율은 항원 97%와 항체 90%로 나타났다. 항원검사의 ICA법 민감도, 특이도, 양성예측도, 음성예측도는 각각 97%, 100%, 100% 및 96.8%로 나타났으며, 항체검사는 각각 90%, 93.3%, 93.1% 및 90.3%로 나타났다. ICA법을 임상에서 사용 시 결과판독에 주의가 필요하며, 낮은 역가의 경우 검출되지 않는 제한점이 있으므로 예민도가 높은 다른 검사법을 이용하여 이중검사를 시행해야 할 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제17권10호
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• pp.1629-1637
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• 2007

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

• 한국식품과학회지
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• 제39권3호
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• pp.299-303
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• 2007

본 연구에서는 L. monocytogenes를 신속하게 분석할 수 있는 면역크로마토그래피법을 개발하고자 하였다. 먼저 L. monocytogenes에 대한 단크론성 항체(FKLM-3B12-37)를 개발하여 직경 40nm로 제작된 colloidal gold와 합성한 후 conjugate pad에 처리하였고 nitrocellulose membrane 상의 test line과 control line에 단크론성 항체와 anti-mouse IgG를 각각 처리하여 면역크로마토그래피법을 개발하였다. 개발된 면역크로마토그래피법의 검출한계는 $10^{5}$ cell/mL 수준까지 검출이 가능했으며 다른 식중독 세균과는 교차 반응이 없는 것으로 나타났다. 임으로 오염시킨 식품시료의 경우 증균과정 없이 $10^{4}$ cell/25g까지 바로 분석하는데 어려움이 있으며 24시간의 증균과정을 거친 후에는 $10^{4}$ cell/25g까지 검출할 수 있었다. 본 연구에서 개발된 면역크로마토그래피법은 24시간의 증균 만으로 식품에 오염되어있는 L. monocytogenes를 검출할 수 있는 것으로 사료되었고, 기존의 식중독균 진단방법에 비해 신속하고 간편하게 그 결과를 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1152-1161
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• 2007

An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L. monocytogenes was produced from cloned hybridoma cells (FKLM-3B12-37) and used to develop an ICG strip test. The antibody showed a stronger binding to L. monocytogenes than other Listeria species, and a weak cross-reaction to S. aureus based on an ELISA. The detection limit of the ICG strip test was $10^5\;cell/ml$. In total, 116 meat and processed-meat samples were collected and analyzed using both the ICG strip test and a PCR. The ICG strip test and PCR indicated L. monocytogenes contamination in 34 and 27 meat samples, respectively. The 7 meat samples not identified as L. monocytogenes positive by the PCR were also tested using an API kit and found to be contaminated by Listeria species. In conclusion, the ICG strip test results agreed well with those obtained using the PCR and API kit. Thus, the developed ICG has potential use as a primary screening tool for L. monocytogenes in various foods and agricultural products, generating results within 20 min without complicated steps.

• 한국토양비료학회지
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• 제45권6호
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• pp.900-903
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• 2012

Cadmium is known to be very toxic to human health and can be relative easily translocated from soils in plants. Therefore, a rapid method for screening Cd in soils and crops has become more and more important. For this reason, we examined a rapid immunochromatograpy (ICG) test kit which uses antigen-antibody reaction based on immunoassay and chromatography. Soils and rice grains collected from mine waste-contaminated sites were determined for their Cd contents using this kit. For comparison purposes, 0.1 M HCl and ICP-OES were employed as a conventional extraction and determination method. Cadmium contents in rice grains determined using ICG technique were $0.46{\sim}2.39mg\;kg^{-1}$ and Cd contents determined using 0.1 M HCl and ICP-OES were $0.52{\sim}1.97mg\;kg^{-1}$. The correlation between these two Cd contents were statistically significant ($r^2$=0.930). The results of Cd contents in soils also showed a statistically significant relationship between these two methods ($r^2$=0.975). On the basis of these results, ICG technique can be applied to rapidly quantify Cd in crops and soils. However, further research is necessary to apply ICG technique for the field screening.

• 한국산학기술학회논문지
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• 제20권7호
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• pp.127-132
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• 2019

인플루엔자는 인플루엔자 바이러스에 의해 발생하는 급성 호흡기 질환으로 고열, 두통 등을 유발하는 질병이다. 인플루엔자는 특히 변이를 통하여 다 종의 아형을 만들어, 스페인 독감과 같이 수천만 명의 사망자를 내는 등 인류에 심각한 위협을 미치고 있다. 이러한 인플루엔자는 감염 이후에 신속한 진단 검사를 통하여 항바이러스 사용이 필수적인데, 이를 위하여 일반적으로 응급 상황에서 신속하게 진단을 할 수 있는 면역크로마토그래피 기반의 인플루엔자 간이 진단 키트를 사용한다. 본 논문에서는 응급상황에서 준 의료인 등이 인플루엔자 감염이 의심되는 다수의 환자 검진을 가능하게 할 수 있도록 영상 기반의 인플루엔자 판독 기법을 개발한다. 특히 영상 기반의 인플루엔자 판독 시, 판독하는 광원 환경에 따라 발생하는 오류를 최소화하기 위하여 결합 누적 분포 함수 기반의 색상 변환을 통하여 광원의 영향을 최소화하는 알고리즘을 제안한다. 다양한 밝기 변환, 색 온도 등의 환경 조건을 가지는 90개의 실험군에 대하여 본 연구에서 제안하는 알고리즘이 다양한 광원 환경에서 강인함을 확인한다.