Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Profile of the Staphylococcal Exotoxin Gene and its Relation with Canine Atopic Dermatitis

포도알구균의 외독소 유전자 분석과 그 외독소가 개 아토피 피부염에 미치는 영향

  • Nam, Eui-Hwa (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Chung, Tae-Ho (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Ji-Hyun (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Park, Seol-Hee (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Hyo-Eun (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Youn, Hwa-Young (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Chae, Joon-Seok (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Park, Yong-Ho (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Hwang, Cheol-Yong (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • 남의화 (서울대학교 수의과대학.수의과학연구소) ;
  • 정태호 (서울대학교 수의과대학.수의과학연구소) ;
  • 김지현 (서울대학교 수의과대학.수의과학연구소) ;
  • 박설희 (서울대학교 수의과대학.수의과학연구소) ;
  • 김효은 (서울대학교 수의과대학.수의과학연구소) ;
  • 윤화영 (서울대학교 수의과대학.수의과학연구소) ;
  • 채준석 (서울대학교 수의과대학.수의과학연구소) ;
  • 박용호 (서울대학교 수의과대학.수의과학연구소) ;
  • 황철용 (서울대학교 수의과대학.수의과학연구소)
  • Accepted : 2011.04.04
  • Published : 2011.04.30
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Abstract

Staphylococcus spp. is one of the most common bacteria isolated from the lesions of atopic dermatitis (AD) in humans, and their colonization is known to be a possible trigger factor of clinical signs. The aim of this study was to determine the prevalence of Staphylococcus spp. in canine AD (CAD), the types of exotoxins present, and their relation with the clinical severity of CAD. From 79 dogs with AD, 72 samples of Staphylococcus spp. were isolated (91.1%), and 65 (90.3%) were confirmed as Staphylococcus pseudintermedius. Concerning the profile of the exotoxin gene, 50 isolates (69.4%) contained at least one exotoxin gene, and 28 isolates (56%) were found to contain more than 2 different exotoxins. There was a significant difference in clinical severity with the presence of staphylococcal exotoxins (P=0.028), whereas no correlation was found with the presence of Staphylococcus spp. (P=0.598). The clinical severity of CAD increased only in relation to staphylococcal enterotoxin D (SED) and exfoliative toxins (P<0.05). Some clinical evaluation criteria (erythema, papule/pustule) were correlated with the presence of the exotoxin gene (P<0.05). This study showed that the high prevalence of Staphylococcus spp. and staphylococcal exotoxins in lesions from dogs with AD may be regarded as an important trigger factor for exacerbation of the clinical signs of CAD.

포도알구균은 아토피 피부염 환자의 피부병변에서 가장 많이 발견되는 세균으로, 이들 균집락의 정도는 사람 아토피 피부염의 임상증상 악화요인으로 알려져있다. 이에 본 연구에서는 개 아토피 피부염 환자의 피부에서 포도알구균의 존재를 확인하고, 이들 균주가 생산하는 외독소 유형을 분석하여 개 아토피 피부염 환자의 임상증상과의 연관성을 알아보았다. 79마리의 개 아토피 피부염 환자 중 91.1%인 72마리에서 포도알구균이 검출되었으며, 이 중 62마리에서 Staphylococcus pseudintermedius가 가장 높은 빈도로 확인되었다. 외독소 유전자 분석에서는 69.4%인 50마리에서 1가지 이상의 외독소 유전자를 포함하였고, 이들 중 56%인 28마리에서 2가지 이상의 다른 외독소 유전자를 가지고 있는 것으로 나타났다. 개 아토피 피부염 환자를 포도알구균의 존재 유무에 따라 분류하였을 때, 임상증상 점수의 차이에 통계적인 의미는 없었지만 (P=0.598), 외독소 유무에 따라 임상증상 점수를 비교하였을 때는 의미있는 차이를 보였다 (P=0.028). 또한 외독소 유형에 따라 분류하였을 때 외독소 중 SED와 exfoliative toxins에서 임상증상에 의미있는 차이를 보였으며 (P<0.05), 외독소 유무에 따라 분류하였을 때는 임상증상 점수 중 특히 발적과 구진/농포에서 의미있는 차이를 보였다 (P<0.05). 이와 같은 결과를 통해 개 아토피 피부염에서 포도얄구균이 생산하는 외독소가 개 아토피 피부염의 증상악화와 관련이 있는 것으로 사료된다.

Keywords

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