• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.2
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• pp.71-76
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• 2006

Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by flowing to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was 100% & 10%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The Present author think that Coptidis rhizoma extract must be made a new eye drops.

• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.99-103
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• 2006

Objective : This experimental study was performed to investigate the effect of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\beta}^{\circ}$ according to density Hwangryunhaedoktang Herbal-acupuncture & extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone With change of volume. Results : 1. MIC on Staphylococcus aureus in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. Conclusions : The present author think that Hwangryunhaedoktang Herbal-acupuncture must be extract a new method for anti-bacterial potency.

• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.73-77
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• 2006

Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture(100%, 10%, 1%, 0.1%). Antibacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture disappear all sample. Conclusions : The present author think that Soyum Herbal-acupuncture must be extract a new method for anti-bacterial potency.

• Journal of Pharmacopuncture
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• v.13 no.1
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• pp.45-52
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• 2010

Objectives : This experimental study was performed to investigate the effectiveness of Fel Ursi Pharmacopunture solution(FUPS) manufactured by using alcohol/water extraction method. To identify the use of it as eyedrops, antibacterial test on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans was performed. Methods : After treatment FUPS on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, we investigated anti-bacterial effects of FUPS on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans in terms of measuring MIC and size of inhibition zone respectively. Results : After FUPS was treated, significant changes of MIC on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans were not observed at all. Conclusions : The present study suggests that FUPS doesn't have anti-bacterial effects on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans which cause Keratitis. Perhap These results recommend that FUPS doesn't have anti-bacterial effects but have other mechanism which suppress inflammation.

• Journal of Microbiology
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• v.39 no.4
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• pp.321-325
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• 2001

Baird-Parker agar with egg yolk/tellurite emulsion (BPA) is widely accepted as a medium for the enu-meration of Staphylococcus aureus in foods. Howerver, it is not vompletely selective and colonies of other genera of species could be similar to those of Staphylococcus aureus. Moreover, the strains of Staphylococcus aureus that are lecithinase negatrive could go unnoticed. Both facts could affect the counts. The aim of this study was to dtermine whether the enumeration of the colonies with the typical morphol-ogy of Staphylococcus aureus on BPA is sufficien to quantity this species in poultry meat. Forty chiken carcasses were tested for Staphylococcus aureus by surface plating using BPA, Results indicate that the predictiv value of the morphoogy of the colonies of BPA is 85.71% and 68.42% for typical and atypical colonies of Staphylococcus aureus, respectively. However, Staphylococcus aureus counts (after identification) and counts of typical colonies did not show any significant differences(P>0.05) and are significantly(P<0.001) correlated (r=0.996).These results suggest that , for screening purposes. enumeration of Staphylococcus aureus from poultry meat does not require any identification of stains. resulting in a saving of time and money.

• Journal of Microbiology and Biotechnology
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• v.30 no.3
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• pp.341-351
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• 2020

To shed light on the genetic differences among food-originated coagulase-negative Staphylococcus (CNS), we performed pan-genome analysis of five species: Staphylococcus carnosus (two strains), Staphylococcus equorum (two strains), Staphylococcus succinus (three strains), Staphylococcus xylosus (two strains), and Staphylococcus saprophyticus (one strain). The pan-genome size increases with each new strain and currently holds about 4,500 genes from 10 genomes. Specific genes were shown to be strain dependent but not species dependent. Most specific genes were of unknown function or encoded restriction-modification enzymes, transposases, or prophages. Our results indicate that unique genes have been acquired or lost by convergent evolution within individual strains.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.17 no.1
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• pp.143-153
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• 2004

Antimicrobial activities against Streptococcus mutans, Candida albicans, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus were assayed through the hot water extracts and the ethanol extracts from Chungyulsodokeum and its composition oriental medicines. The stains were incubated on culture medium and activated. We observed the size of inhibition zone on the strains that is incubated in strilized filter paper disc with various concentration extracts of Chungyulsodokeum and its composition 1. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, Gardeniae Fructus showed antimicrobial activities against Staphylococcus aureus. 2. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Forsythiae Fructus Ghycyrrhizae Radix, showed antimiaobial activities against Staphylococcus epidermidis. 3. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, showed antimicrobial activities against Streptococcus mutans. 4. The extract of Coptidis Rhizoma showed antimicrobial activities against Candida albicans. 5. None of the extracts showed antimicrobial activities against Escherichia coli. We observed antimicrobial activities of Chungyulsodokeum and its composition against Streptococcus mutans, Staphylococcus epidermidis, Staphylococcus aureus.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.1 s.32
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• pp.195-200
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• 2007

Objective : This experimental study was performed to investigate the effect of Jinpi-san on Staphylococcus aureus(S. aureus) and Staphylococcus epidermidis(S. epidermidis) that induce keratitis. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Jinpi-san(100%, 10%, 1%, 0.1 %). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on S. aureus in Jinpi-san was $40{\mu}l$ undiluted solution. 2. MIC on S. epidermidis in Jinpi-san was $20{\mu}l$ undiluted solution. Conclusions : These results indicate that Jinpi-san can be used to cure S. aureus, S. epidermidis that induce eye disease(keratitis). If further study is performed, the use of eye drops will be valuable and beneficial in the clinical medicines.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.2
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• pp.76-85
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• 2007

The total number of clinical materials collected from Dec 1, 2005 to Nov 30, 2006 in C. hospital was 63,133. Among these materials, the isolation of Staphylococcus spp. was 5,252 strains. The proportions of Staphylococcus species infection were as follows ; 46.7% in S. aureus, 39.3% in S. epidermidis, 4.7% in S. hominis, 3.8% in S. haemolyticus, 2.9% in S. capitis and 2.5% in others. Seventeen strains of Staphylococcus species were identified with an isolation range of 0.02-46.7%. The isolation ratios of Staphylococcus species were 7.8% (2,134/27,362) in female, 8.7% (3,118/35,807) in male and 8.3% (5,252/63,133) in both. The age-related frequencies of Staphylococcus species were 22.1% in the 60-69 year group, 19.3% in the 70-79 year group and 17.1% in the 50-59 year group. The isolation ratio of the 0-49 year group to the 50-over 80 year group were 1.74 times in female, 1.92 in male and 1.85 in both. The isolation frequency of Staphylococcus spp. was at its highest in April (9.9%) and at its lowest in February (6.8%) according to monthly analysis. According to seasonal variation, the isolation frequencies of Staphylococcus spp. were 27.0% in Summer, 25.9% in Spring, 23.9% in Fall and 23.2% in Winter. The clinical departments showing over 9% isolation rate against Staphylococcus spp. were critical care medicine (12.9%), department of pediatrics (11.6%), department of urology (9.8%) and department of neuro-surgery (9.1%). On the other hand, the lowest number was observed in the department of family medicine. The clinical material showing over 15% isolation rate against Staphylococcus spp. were 32.1% in urine (random urine and catheterized urine), 20.6% in blood and 16.0% in sputum.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.1
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• pp.11-20
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• 2006

Objective : This experimental study was performed to investigate the effect of herbal eye drops, Sean-tang and Coptidis rhizoma on Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa keratitis. Methods : After administering herbal eye drops (Sean-tang, Coptidis rhizoma) on Staphylococcus ausreus, Staphylococcus. epidermidis and Pseudomonas aeruginosa, I measured MIC and the size of inhibition zone. MIC was measured by dropping to $50{\mu}{\ell}$ according to density(l00%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Result : On Staphylococcus aureus, Staphylococcus epidermidis, Coptidis rhizoma showed the highest anti-bacterial potency and on Pseudomonas aeruginosa, Sean-tang, Coptidis rhizoma was nor anti-bacterial potency. Conclusions : The present author think that the herbal eye drops, Sean-tang and Coptidis rhizoma can be used to cure Staphylococcus aureus, Staphyloccus epidermidis keratitis and if further study is performed, the use of the herbal eye drops will be valuable and beneficial in the clinical medicines.