농업생명과학연구 (Journal of agriculture & life science)

  • 제43권3호
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  • Pages.15-26
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  • 2009
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  • 1598-5504(pISSN)
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  • 2383-8272(eISSN)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
권호 표지

Trichoderma 속에 대한 낙엽송 및 미송 추출물의 항균활성

Antifungal Activity of Wood Extracts of Larix leptolepis and Pseudotsuga menziesii againstTrichoderma spp.

  • 김지수 (경상대학교 환경산림과학부(농업생명과학연구원)) ;
  • 여희동 (경상대학교 환경산림과학부(농업생명과학연구원)) ;
  • 정지영 (경상대학교 환경산림과학부(농업생명과학연구원)) ;
  • 남정빈 (경상대학교 환경산림과학부(농업생명과학연구원)) ;
  • 김지운 (경상대학교 환경산림과학부(농업생명과학연구원)) ;
  • ;
  • 최명석 (경상대학교 환경산림과학부(농업생명과학연구원)) ;
  • 양재경 (경상대학교 환경산림과학부(농업생명과학연구원))
  • Kim, Ji-Su (Div. of Environmental Forest Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Yeo, Hee-Dong (Div. of Environmental Forest Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Jung, Ji-Young (Div. of Environmental Forest Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Nam, Jung-Bin (Div. of Environmental Forest Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Kim, Ji-Woon (Div. of Environmental Forest Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Rinker, Danny Lee (Department of Plant Agriculture, University of Guelph) ;
  • Choi, Myung-Suk (Div. of Environmental Forest Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Yang, Jae-Kyung (Div. of Environmental Forest Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.)
  • 투고 : 2009.06.17
  • 심사 : 2009.06.20
  • 발행 : 2009.06.30

⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 국내산 침엽수 톱밥추출물을 이용하여 버섯의 푸른곰팡이병 방제약제 원료를 개발하고자 시도 되었으며, 푸른곰팡이병의 원인균의 하나인 Trichoderma 속 곰팡이를 대상으로 항균활성을 시험하였다. 1,000 ppm 농도의 낙엽송 열수추줄물은 Trichoderma 속 균주에 대하여 최고 20.6%의 항균활성을 나타냈다. 1,000 ppm 농도의 미송 열수추출물은 T. aggressivum에 대하여 60.3%, T. harzianum 52.2%, T. atroviride 46.8%, T.viride 46.1%의 높은 항균활성을 나타냈으며 T. koningii에서는 36.2%의 항균활성을 나타냈다. 미송 열수출물이 낙엽송 열수출물보다 Trichoderma 속 균주에 대하여 보다 효과적인 항균화합물을 함유하고 있다고 판단되었다. 낙엽송 열수출물로부터 분리된 n-hexane 분획물은 Trichoderma 속에 대하여 68.5% ~ 79.9%의 높은 항균활성을 나타냈다. 열수출물로부터 분리된 미송의 n-hexane 분획물은 T. aggressivum에 대하여 68.5%, T. atroviride에 대하여 71.4%, T. harzianum에 대하여 71.9%, T. koningii에 대하여 75.7%, T.viride에 대하여 82.3%의 높은 항균활성을 나타냈고, ethyl acetate 분획물이 n-hexane 분획물 다음으로 높은 항균활성이 나타내었다. Trichoderma 속 균주에 대한 낙엽송 및 미송 열수추출물의 항균활성은 미송 추출물이 낙엽송 추출물보다 높게 나타났지만, 낙엽송과 미송의 열수추출물로부터 분리된 n-hexane 분획물에 의한 항균활성 차이는 거의 없었다. 미송 열수추출물과 낙엽송 및 미송의 열수출물로부터 획득된 n-hexane 분획물은 Trichoderma 속에 대한 항균원료로서 사용가능성을 확인할 수 있었다.

This study was undertaken to determine inhibitory compounds from extracts of the softwood (larix leptolepis, Pseudotsuga menziesii) sawdust against Trichoderma spp. The sawdust of L. leptolepis and P. menziesii were hot water extracted, which were with fraction extracted organic solvents. The organic solvent extractions were carried out by n-hexane, methylene chloride, ethyl acetate. The antifungal activity of hot water extracts of L. leptolepis sawdust was determined to be 20.6% inhibition at a concentration of 1,000 ppm against Trichoderma spp. The antifungal activity of P. menziesii sawdust was outstanding about 60.3% against Trichoderma spp. The yields of the fractions of n-hexane soluble, methylene chloride soluble and ethyl acetate soluble from the hot water extract of L. leptolepis sawdust were 4.0%, 6.0% and 8.0%, repectively. However, the yields of the fractions of three solvents of P. menziesii sawdust were 8.0%, 13.0 and 14.0% correspondingly. The antifungal activity of n-hexane soluble fraction from hot water extracts of L. leptolepis sawdust was highest to about 68.5% to 79.9% against Trichoderma spp. compared to others. The antifungal activities of n-hexane soluble fraction from hot water extracts of P. menziesii sawdust showed 68.5%, 71.4%. 71.9%, 75.7% and 82.3% against T. aggressivum, T. atroviride, T. harzianum, T. koningii and T.viride, respectively. The n-hexane soluble fraction revealed much higher antifungal activity than the other fractions did. This study demonstrated that the n-hexane fraction of the hot water extracts of L. leptolepis and P. menziesii exhibited the greatest antifungal activity against Trichoderma spp.

키워드

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