Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Production of Spirulina Extract by Enzymatic Hydrolysis

효소 가수분해 방법을 이용한 스피루리나 추출물의 제조

  • In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Gwon, Su-Yeon (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Chae, Hee-Jeong (Department of Food and Biotechnology, Hoseo University) ;
  • Kim, Dong-Chung (Institute of Basic Science, Sungkyunkwan University) ;
  • Kim, Dong-Ho (FNBio Co., Ltd.)
  • Published : 2007.12.31
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Abstract

An efficient production method of spirulina extract was developed by enzymatic treatment using cell lytic and proteolytic enzymes. The suitable dosage of Tunicase, a cell lytic enzyme, was found to be 2.0% (w/w). Proteolytic enzymes were screened to obtain high solid recovery and spirulina extraction (SE) index, which indicates nucleic acid-related substances content. Among the seven tested proteases, Esperase was selected and optimal dosage of this enzyme was 2.0% (w/w). The solid recovery and SE index of simultaneous treatment and co-treatment using optimal dosages of Tunicase and Esperase were greatly similar, respectively. However, co-treatment had the effect of shortening total hydrolysis time. The SE index and solid recovery of co-treatment were significantly enhanced by 75% $(11.4{\rightarrow}20.0)$ and 45% $(45.2%{\rightarrow}65.3%)$, respectively, than those of the non-treated extracts.

세포벽 분해 효소와 단백질 분해 효소를 이용하여 스피루리나 추출물을 효율적으로 생산할 수 있는 방법을 조사하였다. 세포벽 분해 효소인 Tunicase의 사용 농도는 2%가 적당하였다. 고형분 회수율과 핵산 관련 성분의 함량을 나타내는 spiruina extraction(SE) index를 기준으로 상업용 단백질 분해 효소를 선별하였다. 일곱 종류의 효소를 조사한 결과, Esperase가 가장 우수하였으며, 최적 사용량은 2%이었다. Tunicase와 Esperase를 순차전으로 반응시키거나 동시에 반응시켜도 고형분 회수율과 SE index는 매우 유사하였으며 동시에 사용하는 것이 반응 시간을 단축시킬 수 있었다. 두 효소를 동시에 반응시키면 단순 열수 추출보다 고형분 회수율은 약 45%$(45.2%{\rightarrow}65.3%)$, SE index는 약 75%$(11.4{\rightarrow}20.0)$ 증가하였다.

Keywords

References

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