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Production of Spirulina Extract by Enzymatic Hydrolysis  

In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University)
Gwon, Su-Yeon (Department of Human Nutrition and Food Science, Chungwoon University)
Chae, Hee-Jeong (Department of Food and Biotechnology, Hoseo University)
Kim, Dong-Chung (Institute of Basic Science, Sungkyunkwan University)
Kim, Dong-Ho (FNBio Co., Ltd.)
Publication Information
Applied Biological Chemistry / v.50, no.4, 2007 , pp. 304-307 More about this Journal
Abstract
An efficient production method of spirulina extract was developed by enzymatic treatment using cell lytic and proteolytic enzymes. The suitable dosage of Tunicase, a cell lytic enzyme, was found to be 2.0% (w/w). Proteolytic enzymes were screened to obtain high solid recovery and spirulina extraction (SE) index, which indicates nucleic acid-related substances content. Among the seven tested proteases, Esperase was selected and optimal dosage of this enzyme was 2.0% (w/w). The solid recovery and SE index of simultaneous treatment and co-treatment using optimal dosages of Tunicase and Esperase were greatly similar, respectively. However, co-treatment had the effect of shortening total hydrolysis time. The SE index and solid recovery of co-treatment were significantly enhanced by 75% $(11.4{\rightarrow}20.0)$ and 45% $(45.2%{\rightarrow}65.3%)$, respectively, than those of the non-treated extracts.
Keywords
enzymatic hydrolysis; solid recovery; spirulina extract;
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Times Cited By KSCI : 5  (Citation Analysis)
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