• Title/Summary/Keyword: solid recovery

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Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S

  • Shata, Hoda Mohamed Abdel Halim;Farid, Mohamed Abdel Fattah
    • Journal of Applied Biological Chemistry
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    • v.55 no.3
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    • pp.149-156
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    • 2012
  • The effects of solvent type and concentration, solid/liquid ratio, extraction time and repeated extraction on recovery of keratinase from solid-state fermentation (SSF) of chicken feather by a local Streptomyces sp. NRC 13S were investigated in order to establish the experimental conditions for keratinase yield. Among solvents tested, 0.5% (v/v) glycerol was the best. Box-Behnken design was used to investigate the effect of relevant variables on keratinase recovery. The factors investigated were solid/liquid ratio (1:1.66-1:6.66 g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The results showed that the maximum recovery of keratinase (6933.3 U/gfs) was obtained using 0.5 (v/v) glycerol as extracting solvent, in a solid/liquid ratio of 1:5 and three extraction cycles.

Enhancing Extraction Yield of Chlorella Extract by Enzyme Treatment

  • In, Man-Jin;Jang, Jae-Eun;Kim, Dong-Ho
    • Journal of Applied Biological Chemistry
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    • v.50 no.3
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    • pp.132-135
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    • 2007
  • An efficient production method of chlorella extract was developed by enzymatic treatment using cell lytic and proteolytic enzymes. The suitable dosage of Tunicase, a cell lytic enzyme, was found to be 1.0% (w/w). Proteolytic enzymes were screened to obtain high chlorella growth factor (CGF) index, which indicates crude CGF content and solid recovery. Among the seven tested proteases, Esperase, whose optimal dosage was 1.0% (w/w), was selected. By co-treatment using optimal dosages of Tunicase and Esperase, the highest CGF index and solid recovery were obtained. The CGF index and solid recovery of co-treatment were remarkably enhanced by 250 ($4.36{\rightarrow}15.21$) and 220% ($12.65%{\rightarrow}40.15%$), respectively, than those of the non-treated extracts.

Application of Reversed-Phase Solid Phase Extraction for the HPLC Analysis of Microcystins in Water (다량 시료중 마이크로시스틴의 농축 및 분석)

  • Kim, Myeong-Hee;Kim, Tae-Seung;Kim, Tae-Keun;Park, Sun Ku
    • Analytical Science and Technology
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    • v.13 no.3
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    • pp.399-402
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    • 2000
  • To determine the concentrations of microcystins present in lake water or in tap water using high performance liquid chromatography, it is necessary to concentrate a large volume of water samples (about 20 L) into very small volume (0.1-0.3 mL). Concentration can be conveniently done when disc type solid phase extraction (SPE) apparatus is used. Using this apparatus we have investigated the recovery rates of three kinds of microcystins, RR, YR, LR. The recovery rates were relatively low and the reproducibilities were not good either. It is expected, however, that the appropriate selection of the disc conditioning and eluting solvents and reproducible reconcentration process after SPE will improve both the recovery rates and the reproducibilities.

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Optimization of Proteolytic Enzyme Treatment for the Production of Spirulina Extract (단백질 분해 효소를 이용한 스피루리나 추출물 제조 공정 최적화)

  • In, Man-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.2
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    • pp.550-555
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    • 2008
  • An efficient production method of spirulina extract was developed by enzymatic treatment using proteolytic enzymes. The suitable dosage of Tunicase, a cell lytic enzyme, was used to be 2.0% (w/w). To maximize solid recovery and spirulina extraction (SE) index, which indicates nucleic acid-related substances content, the dosage of Alcalase, commercially available pretense, was found to be 1.0% (w/w). By simultaneous treatments using optimal dosages of Tunicase and Alcalase, the highest SE index and solid recovery were obtained. The SE index and solid recovery of simultaneous treatments were notably enhanced by 100% ($11.4%\;{\rightarrow}\;22.8%$) and 56% ($45.2%\;{\rightarrow}\;70.7%$), respectively, than those of the non-treated extracts.

β-Glucosidase Recovery from a Solid-State Fermentation System by Aspergillus niger (Aspergillus niger 의 고체상태 발효 시스템에서의 β-Glucosidase 회수)

  • Chandra, M. Subhosh;Reddy, B. Rajasekhar;Choi, Yong-Lark
    • Journal of Life Science
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    • v.20 no.7
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    • pp.999-1004
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    • 2010
  • Investigations were carried out on a $\beta$-glucosidase produced by Aspergillus niger under solid-state fermentation conditions as a model of enzyme recovery from fermented wheat bran. The leaching efficiency of distilled water to recover the enzyme from the fermented bran was higher than acetate buffer, citrate buffer, citrate-phosphate buffer and 5% methanol; thus, the conditions were further optimized with distilled water as the extracting agent. After fermented bran was washed three times with distilled water for 1.5 hr each under shaking conditions at 1:5 solid to solvent ratio, a maximum recovery of 0.025 U/g of wheat bran was obtained.

Problem and Optimum Operational Strategy of Multipurpose Reservoir in Korea (우리나라 다목적 Dam 운영의 문제점과 개선방안)

  • 심순보
    • Journal of the Korean Professional Engineers Association
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    • v.19 no.1
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    • pp.25-32
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    • 1986
  • The number of visiters to Bukhan Mt. national park, generation quantity of solid waste and collection system were researched to consider a counterplan for the pollution control of the national park and study for developing the effective treatment of solid waste was tried through the proximate analysis of each component containing. Results obtained in this study were summerized as follows; The great part of visitors go on an excursion to the Bukhan Mt. national park during July and August and also, the solid waste was generated nearly a half of the total amount at the same period. The major collection facilities in the national park were waste basket and incineration box. But the incineration box was too large in volume and very far in distance, and its collection period was irregular, so it was cause to the congestion of solid waste and bad smell and dirty. Therefore, to complete collection of solid waste, we must set up the waste basket which able to find within 40~50m from the origination place of solid waste and induce the visitors to throw the solid waste. It was obtained as moisture content: 48.5 wt%, volatile solid: 28.4wt%, fixed solid: 23.1 wt%, lower heating value: 1,320kca1/kg from experimental analysis of solid waste. According to this analysis, the incineration operation is possible, but the generation quantity of solid waste was too small to construct incineration plant for heat recovery. It was found that it is suitable for the aerobic composting by mixing with the night soil which generate in the national park after the recovery of resources such as metals, glasses and plastics.

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Discussion of Current Resource Recycling Policy in Taiwan

  • Chen, Shiao-Shing;Chang, Tien-Chin;Huang, Cheng-Yi
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.675-679
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    • 2001
  • The research is to discuss the current resource recycling and recovery policy, which was enacted by Environmental Protection Administrative (EPA) in Taiwan. For the past few years, the solid waste generated in Taiwan has greatly increased about 5 % per year. In addition to the construction of landfill sites and incineration plants, 4 R techniques (Reduction, Reuse, Recycle and Recovery) were also publicized among the citizens and then promulgated to furthermore manage these increased solid waste. Although the regulations have been carried out to a great success, they still need to be revised and updated since solid waste contains varieties of different materials. Therefore, this research discusses the current regulation and makes suggestion for future regulation revision. From the results of this study, energy recovery was suggested to be emphasized in the regulation. Energy could be recovered from materials such as waste tires, and all kinds of plastic containers. Waste tires and most of the plastic containers made of hydrocarbon species, which contains great heating values, should be considered as one of the alternatives for the resource recycling.

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Hemicellulose Recovery from Lignocellulosic Material Hydrolyzed by Water (물로 가수분해된 섬유성 기질로부터 hemicellulose 회수)

  • Kim, Sung-Bae;Kim, Chang, Joon
    • KSBB Journal
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    • v.20 no.4
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    • pp.317-322
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    • 2005
  • Various recovery methods were investigated to maximize hemicellulose recovery from lignocellulosic material hydrolyzed by pure water. The pretreatment conditions of water hydrolysis were $170\~180^{\circ}C$ and 1 hour of reaction time. The percentage of hemicellulose solubilized increased as the temperature increased from 170 to $180^{\circ}C$. However, significant decomposition of sugar was observed at temperature of $180^{\circ}C$. From the results of water hydrolysis, the total amount of glucan in solid residue and liquid hydrolyzate was close to the total glucan in the original biomass. For hemicellulose, however, there was a significant difference between both contents. To prove this difference, various recovery methods were proposed. From the total sugar accountability (sugar in liquid + sugar in solid), it was confirmed that hemicellulose recovery in the hydrolyzate was increased if the product including both hydrolyzate and solid residue was physically stimulated by such as heating and ultrasound irradiation. This indicated that, in commercial scale processes that much bigger substrate sizes are used and a sufficient amount of leaching solvent can not be used after pretreatment, a significant amount of oligomers could be trapped in the solid matrix.

Hydrothermal carbonization of sewage sludge for solid recovered fuel and energy recovery (수열탄화를 이용한 하수 슬러지의 고형연료화 및 에너지 회수 효율)

  • Kim, Daegi;Lee, Kwanyong;Park, Kiyoung
    • Journal of Korean Society of Water and Wastewater
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    • v.29 no.1
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    • pp.57-63
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    • 2015
  • Recently, Korea's municipal wastewater treatment plants generated amount of wastewater sludge per day. However, ocean dumping of sewage sludge has been prohibited since 2012 by the London dumping convention and protocol and thus removal or treatment of wastewater sludge from field sites is an important issue on the ground site. The hydrothermal carbonization is one of attractive thermo-chemical method to upgrade sewage sludge to produce solid fuel with benefit method from the use of no chemical catalytic. Hydrothermal carbonization improved that the upgrading fuel properties and increased materials and energy recovery, which is conducted at temperatures ranging from 200 to $350^{\circ}C$ with a reaction time of 30 min. Hydrothermal carbonization increased the heating value though the increase of the carbon and fixed carbon content of solid fuel due to dehydration and decarboxylation reaction. Therefore, after the hydrothermal carbonization, the H/C and O/C ratios decreased because of the chemical conversion. Energy retention efficiency suggest that the optimum temperature of hydrothermal carbonization to produce more energy-rich solid fuel is approximately $200^{\circ}C$.

A Study on Analytical Method of Phthalate Esters in Water by SPE(Solid-Phase-Extraction) (고체상 추출법을 이용한 Phthalate Esters의 분석방법 연구)

  • 홍성희;한개희;이찬형;이순화
    • Journal of Environmental Science International
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    • v.12 no.1
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    • pp.81-86
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    • 2003
  • The study was carried out to evaluate the new analytical method of phthalate esters(diethylphthalate, di-n-butylphthalate, butylbenzylphthalate, bis(2-ethylhexyl)phthalate), one of the endocrine disruptors, which were performed by GC/MS-SIM(selected ion monitoring). The phthalate esters were extracted from water samples using solid-phase extraction on $C_{18}$ columns. It investigated that the extraction recovery rate of phthalate esters with different solvents and solvent volume. The optimal solvent was dichloromethane and proper volume of dichloromethane for recovery of phthalate esters was 4 mL. There were good linearities(above $R^2$=0.9975) in the range 0.01~0.50mg/L, and the detection limits were below 0.01~0.03$\mu\textrm{g}$/L. The recovery rates, RSD and MDLs for phthalate esters were 80~114%, 5.0~8.1% and 0.03~0.11$\mu\textrm{g}$/L, respectively. This method shows a good precision of phthalate esters.