Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of the Concentration of Ammonia in Maturation Medium on the Development and Cell Numbers of Korean Native Cow Embryos

한우 난포란의 체외성숙 배지 내의 암모니아 농도가 배 발생과 세포수에 미치는 영향

  • Published : 2005.03.01
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Abstract

The purpose of this study was an improvement of efficiency and quality in the production of Korean Native Cow embryos. We investigated effects of concentration of ammonia in in vitro maturation (IVM) medium. In addition, we examined effects of addition or exchange of IVM medium on subsequent development and the cell numbers of blastocysts. The concentrations of ammonia in IVM medium was significantly increased by the increasement of IVM duration (p<0.05). The development rates to the 2 cell-, 8 cell- and blastocyst-stage embryos with the addition of IVM medium were similar among treatment groups. The number of inner cell mass (ICM) cells and the total cell number (TCN) of blastocysts were not differ among treatment groups, whereas the trophectoderm (TE) cell number was significantly lower in the group of 4.5 h addition. The ICM/TCN ratio was significantly higher in the group of 4.5 h addition than in the group of control and 9 h addition. The development rate to the 2-cell embryo with the exchange of IVM medium was significantly higher in the group of 4.5 h exchange and 9 h exchange than in control. The development rate to the blastocyst stage was the highest in the group of 9 h exchange. The number of ICM and ICM/TCN ratio were significantly higher in the group of 9 h exchange than the other groups. The numbers of TE and TCN was similar among treatment groups.

본 연구는 한우 수정란의 체외생산에 있어서 효율과 품질의 향상을 위해서, 체외성숙 시간에 따른 배지 내의 ammonia 농도를 측정하였고, 체외성숙용 배지의 교환 및 첨가가 배 발생과 배반포의 세포수에 미치는 효과를 검토하였다. 체외성숙용 배지내의 ammonia 농도는 체외성숙 시간이 길어짐에 따라 유의하게 증가하였다(p<0.05). 체외성숙용 배지의 첨가에 따른 수정율, 8세포기 및 배반포기 발달율은 각 군간에 유사한 경향이었다. 배반포의 inner cell mass(ICM) 및 총 세포수(TCN)는 비슷한 경향이었으나, trophectoderm(TE) 세포수는 4.5 시간 첨가군이 유의하게 낮았다. 한편 ICM/TCN 비율은 4.5 시간 첨가군이 대조군과 9 시간 첨가군에 비하여 유의하게 높았다. 체외성숙용 배지의 교환에 따른 수정율은 4.5시간 및 9시간 교환이 대조군에 비하여 유의하게 높았으나, 8세포기 발달율은 비슷한 경향이었다. 한편 배반포기 발달율은 9 시간 교환군이 가장 높았다. 배반포의 ICM 세포수와 ICM/TCN 비율은 9 시간 교환군이 다른 두 처리군에 비하여 유의하게 높았으나, TE 및 TCN은 차이가 없었다.

Keywords

References

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