Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Reduction of Perchlorate and Nitrate by Citrobacter Amalonaticus Strain JB101 : Kinetics and the Applicability of MBR

Citrobacter Amalonaticus Strain JB101에 의한 과염소산염과 질산염의 환원 : Kinetics 및 MBR을 이용한 처리 가능성

  • Hong, Jae-Wha (School of Environmental and Civil Engineering, Inha University) ;
  • Jang, Myung-Su (School of Environmental and Civil Engineering, Inha University) ;
  • Lee, Il-Su (School of Environmental and Civil Engineering, Inha University) ;
  • Bae, Jae-Ho (School of Environmental and Civil Engineering, Inha University)
  • 홍재화 (인하대학교 공과대학 환경토목공학부) ;
  • 장명수 (인하대학교 공과대학 환경토목공학부) ;
  • 이일수 (인하대학교 공과대학 환경토목공학부) ;
  • 배재호 (인하대학교 공과대학 환경토목공학부)
  • Published : 2005.12.31
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Abstract

This study was performed to evaluate the characteristics of the competition between two electron acceptors, perchlorate and nitrate, with Citrobacter Amalonaticus strain JB101. In addition, the applicability of membrane bioreactor(MBR) for perchlorate removal was evaluated. The maximum growth rate of strain JB101 on perchlorate and nitrate are 0.27 and 0.58 $hr^{-1}$, and maximum substrate utilization rates were 35.1 mg $ClO_4^-/g$ protein-day and 45.6 mg $NO_3^-/g$ protein-day, respectively. Nitrate was a competitive inhibitor for perchlorate, and strain JB101 prefer nitrate to perchlorate as electron acceptor. Complete removal of perchlorate could be achieved up to the surface leading rate of 4.6 g $ClO_4^-/m^2-day$ with the MBR fed with 20 mg $ClO_4^-/L$(HCMBR). When 5 mg/L of nitrate was added to the same influent, perchlorate removal efficiency decreased to 96.5%, while nitrate was completely removed. For the MBR fed with 0.7 mg/L of perchlorate (LCMBR), the maximum perchlorate removal efficiency was 100% up to the loading rate of 0.23 g $ClO_4^-/m^2-day$. Membrane fouling was found to be a problem at high leading rate for both MBRs. The acetate consumption ratio per perchlorate was $13.7{\sim}51.7\;e^-eq./e^-eq.$ in LCMBR, while the value was $2.5{\sim}3.6\;e^-eq./e^-eq.$ in HCMBR. This difference could be related to the acetate consumption with oxygen as electron acceptor. Therefore, the amount of acetate addition must be determined considering the concentrations of other electron acceptors in the influent.

Citrobacter Amalonaticus strain JB101을 이용하여 과염소산염과 질산염이 동시에 존재할 때 두 전자수용체 간의 경쟁특성을 파악하고, MBR(membrane bioreactor)을 이용한 과염소산염 처리 가능성을 검토하였다. 과염소산 및 질산염에 대한 strain JB101의 비성장속도는 각각 0.27 및 0.58 $hr^{-1}$, 최대기질이용속도는 각각 35.1 mg $ClO_4^-/g$ protein-day 및 45.6 mg $NO_3^-/g$ protein-day이었다. 질산염은 과염소산염에 대한 경쟁적 저해제이었으며, strain JB101은 과염소산보다는 질산염을 전자수용체로 선호하였다. 유입 과염소산염 농도가 20 mg/L이였던 HCMBR에서는 막 면적당 부하 4.6 g $ClO_4^-/m^2-day$까지 과염소산염의 제거효율이 100%이었으나, 질산염 5 mg/L를 첨가하자 질산염은 완전히 제거되었으나 과염소산염 제거효율이 96.5%로 감소하였다. 유입 과염소산염 농도가 0.7 mg/L이였던 LCMBR에서는 부하 0.23 g $ClO_4^-/m^2-day$까지 과염소산염의 제거효율이 100%이었다. 두 MBR 모두 높은 부하에서는 막의 막힘현상이 문제로 제기되었다. 과염소산염에 대한 아세트산염 소모비는 LCMBR의 경우 $13.7{\sim}51.7$로 HCMBR의 $2.5{\sim}3.6$보다 높았으며, 이는 아세트산염이 산소를 전자수용체로 이용하여 소모되었기 때문이다. 따라서 전자공여체 첨가량은 유입수에 포함된 다른 전자수용체의 농도를 고려하여 결정하여야 한다.

Keywords

References

  1. Water Res. v.35 no.12 Biological perchlorate reduction in high-salinity solution Logan, B.E.;Wu, J.;Unz, R.F.
  2. Proceedings of the 218th National Meeting of the American Chemical Society as Part of the Environmental Division Symposium: Perchlorate in the Environment Isolation and characterization of two novel (per)chlorate-reducing bacteria from swine waste lagoons Michaelidou, U.;Achenbach, L.A.;Coates, J.D.
  3. Environ. Eng. Sci. v.17 no.5 Fixed-bed bioreactor treating perchlorate contaminated waters Kim, K.J.;Logan, B.E.
  4. Appl. Environ. Microbiol. v.65 no.12 Ubiquity and diversity of dissimilatory (per)chlorate-reducing bacteria Coates, J.D.;Michaelidou, U.;Bruce, R.A.;O'connor, S.M.;Crespi, J.N.;Achenbach, L.A.
  5. Coates, J. D., Michaelidou, U., O'Connor, S. M., Bruce, R. A., and Achenbach, L. A., 'The diverse microbiology of (per)chlorate reduction,' in Proceedings of the 218th National Meeting of the American Chemical Society as Part of the Environmental Division Symposium: Perchlorate in the Environment, the American Chemical Society, Louisiana, pp. 183-195(1999)
  6. Proceedings of the 218th National Meeting of the American Chemical Society as Part of the Environmental Division Symposium: Perchlorate in the Environment The diverse microbiology of (per)chlorate reduction Coates, J.D.;Michaelidou, U.;O'Connor, S.M.;Bruce, R.A.;Achenbach, L.A.
  7. Water Res. v.35 no.13 Microbial reduction of perchlorate in pure and mixed culture packed-bed bioreactors Kim, K.J.;Logan, B.E.
  8. Mikrobiologiya v.45 Bacterial decomposition of ammonium perchlorate Romanceko, V.L.;Korenkov, V.N.;Kuznetsov, S.I.
  9. Treatment of Groundwater Containing Perchlorate Using Biological Fluidized Bed Reactors with GAC or Sand Media;Perchlorate in the Environment Greene, M.;Pitre, M.P.;Urbansky, E.T.(ed.)
  10. Proceedings of the 5th Annual Joint Services Pollution Prevention & Hazardous Waste Management Conference & Exhibition ARA perchlorate treatment experience Copploa, E.N.
  11. Environ. Microbiol. v.1 no.4 Reduction of (per)chlorate by a novel organism isolated from paper mill waste Bruce, R.A.;Achenbach, L.A.;Coates, J.D.
  12. Appl. Microbiol. Biotechnol. v.45 Transformation of (per)chlorate into chloride by a newly isolated bacterium, reduction and dismutation Rikken, G.B.;Kroon, A.G.;van Ginkel, C.G.
  13. Logan, B. E. and LaPoint, D., 'Treatment of perchlorate and nitrate contaminated groundwater in an autotrophic, gas phase, packed-bed bioreactor,' Water Res., 36, 3647- 3653(2002) https://doi.org/10.1016/S0043-1354(02)00049-0
  14. Herman, D. C. and William, T. F., 'Bacterial reduction of perchlorate and nitrate in water,' J Environ. Qual., 28(3), I018-1024(1999)
  15. Water Res. v.38 Chlorate and nitrate reduction pathways are separately induced in the perchlorate-respiring bacterium Dechlorosoma sp. KJ and the chlorate-respiring bacterium Pseudomonas sp. PDA Xu, J.;Trimble, J.J.;Steinberg, L.;Logan, B.E.
  16. Water Res. v.36 Treatment of perchlorate and nitrate contaminated groundwater in an autotrophic, gas phase, packed-bed bioreactor Logan, B.E.;LaPoint, D.
  17. J. Environ. Qual. v.28 no.3 Bacterial reduction of perchlorate and nitrate in water Herman, D.C.;William, T.F.
  18. J. Ind. Microbiol. Biotechnol. v.20 Perchlorate reduction by a mixed culture ？in an up-flow anaerobic fixed bed reactor Wallace, W.;Beshearl, S.;Williams, D.;Hospadarw, S.;Owens, M.
  19. Tchobanoglous, G. and Burton, F. L., Wastewater Engineering; Treatment, Disposal and Reuse, McGraw-Hill, New york, pp. 1275-1279(1991)
  20. Journal of Basic Microbiology v.44 Role of Citrobacter amalonaticus and Citrobacter farmari in dissimilatory perchlorate reduction Bardiya, N.;Bae, J.H.
  21. Standard Methods for the Examination of Water and Wastewater APHA
  22. Anal. Biochem. v.9 A micro-biuret method for estimating proteins Itzhaki, R.F.;Gill, D.
  23. Wastewater Engineering; Treatment, Disposal and Reuse Tchobanoglous, G.;Burton, F.L.
  24. Appl. Environ. Microbiol. v.67 no.6 Kinetics of perchlorate and chlorate-respiring bacteria Logan, B.E.;Zhang, H.;Mulvaney, P.;Milner, M.G.;Head, J.M.;Unz, R.F.
  25. Environ. Pollut. v.118 no.3 Reduction of perchlorate and nitrate by salt tolerant bacteria Benedict, C.O.;Giblin, T.;Frankenberger, W.T.
  26. Mikrobiologiya v.45 Bacterial decomposition of ammonium perchlorate Romanceko, V.L.;Korenkov, V.N.;Kuznetsov, S.I.
  27. J. AWWA v.94 no.11 Perchlorate reduction in a hydrogen-based membrane-biofilm reactor Nerenberg, R.;Rittmann, B.E.;Najm, I.