Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Isolation and PCR detection of Listeria monocytogenes on raw beef and pork carcass

소와 돼지도체에서 Listeria monocytogenes의 분리 및 PCR 검출 방법에 관한 연구

  • Chae, Hee-Sun (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim, Doo-Hwan (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim, Gu-Hyun (Seoul Metropolitan Health & Environment Research Institute) ;
  • Shin, Bang-Woo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Jo, Mi-Yoeng (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kweon, Taek-Boo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Lee, Jung-Hak (Seoul Metropolitan Health & Environment Research Institute)
  • 채희선 (서울특별시보건환경연구원) ;
  • 김두환 (서울특별시보건환경연구원) ;
  • 김규현 (서울특별시보건환경연구원) ;
  • 신방우 (서울특별시보건환경연구원) ;
  • 조미영 (서울특별시보건환경연구원) ;
  • 권택부 (서울특별시보건환경연구원) ;
  • 이정학 (서울특별시보건환경연구원)
  • Published : 2003.06.01
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Abstract

From February 2000 to December 2001, A total of 1,785 samples was taken from beef and pork carcasses in Seoul. Seven(0.69%) Listeria spp. were isolated from the 1,014 of beef carcasses, and five(0.65%) were isolated from the 771 of pork carcasses. The isolates were identified L monocytogenes by API listeria, and VIDAS LMO kit, serological test and PCR assay were preformed. A total 12 strains of L monocytogenes were isolated form samples tested and all of the strains were classified into serotype 1. PCR primers are selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene(hlyA) of Listeria monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested.

Keywords

References

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