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Isolation and PCR detection of Listeria monocytogenes on raw beef and pork carcass  

Chae, Hee-Sun (Seoul Metropolitan Health & Environment Research Institute)
Kim, Doo-Hwan (Seoul Metropolitan Health & Environment Research Institute)
Kim, Gu-Hyun (Seoul Metropolitan Health & Environment Research Institute)
Shin, Bang-Woo (Seoul Metropolitan Health & Environment Research Institute)
Jo, Mi-Yoeng (Seoul Metropolitan Health & Environment Research Institute)
Kweon, Taek-Boo (Seoul Metropolitan Health & Environment Research Institute)
Lee, Jung-Hak (Seoul Metropolitan Health & Environment Research Institute)
Publication Information
Korean Journal of Veterinary Service / v.26, no.2, 2003 , pp. 105-111 More about this Journal
Abstract
From February 2000 to December 2001, A total of 1,785 samples was taken from beef and pork carcasses in Seoul. Seven(0.69%) Listeria spp. were isolated from the 1,014 of beef carcasses, and five(0.65%) were isolated from the 771 of pork carcasses. The isolates were identified L monocytogenes by API listeria, and VIDAS LMO kit, serological test and PCR assay were preformed. A total 12 strains of L monocytogenes were isolated form samples tested and all of the strains were classified into serotype 1. PCR primers are selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene(hlyA) of Listeria monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested.
Keywords
L monocytogenese; PCR; Carcasses;
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