Clinical and Experimental Reproductive Medicine

  • 제29권2호
  • /
  • Pages.97-103
  • /
  • 2002
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

심한 무력정자증 환자의 ICSI 시행시 Pentoxifylline을 사용한 정자처리법이 임상결과에 미치는 영향

Effect of Pentoxifylline on the ICSI Program Undergone in Severe Asthenozoospermia

  • 손지온 (차병원 여성의학연구소) ;
  • 신지수 (차병원 여성의학연구소) ;
  • 정창진 (차병원 여성의학연구소) ;
  • 조용선 (차병원 여성의학연구소) ;
  • 엄기붕 (차병원 여성의학연구소) ;
  • 최동희 (차병원 여성의학연구소) ;
  • 김현주 (차병원 여성의학연구소)
  • Sohn, Jie-Ohn (Infertility Medical Center, CHA General Hospital of Seoul) ;
  • Shin, Ji-Su (Infertility Medical Center, CHA General Hospital of Seoul) ;
  • Jeong, Chang-Jin (Infertility Medical Center, CHA General Hospital of Seoul) ;
  • Cho, Yong-Seon (Infertility Medical Center, CHA General Hospital of Seoul) ;
  • Oum, Ki-Boong (Infertility Medical Center, CHA General Hospital of Seoul) ;
  • Choi, Dong-Hee (Infertility Medical Center, CHA General Hospital of Seoul) ;
  • Kim, Hyun-Joo (Infertility Medical Center, CHA General Hospital of Seoul)
  • 발행 : 2002.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: The aim of this study was to evaluate the effect of pentoxifylline (PF) on the conventional ICSI program undergone in severe asthenozoospermia. Method: Total 348 cycles of ICSI programs undertaken at CHA General Hospital from January, 1996 to September, 2000, were divided into two groups - injected with pentoxifylline-treated sperm (PFT, 204 cycles) or non-treated sperm (NPFT, 144 cycles) and the clinical results of PFT group were compared with those of NPFT. Results: PF-treatment on sperm increased their motility of normozoospermia and severe asthenozoospermia. Fertilization rate of PFT group was higher than those of ICSI programs undertaken using sperm of NPFT (70.6% vs. 62.9%, p<0.01). And, ET and clinical pregnancy rates of PFT were slightly higher than those of NPFT (93.1%, 44.2% vs. 90.3%, 36.2%). Conclusion: These results showed that treatment of pentoxifylline has a beneficial role on selection of viable sperm in severe asthenozoospermia.

키워드

참고문헌

  1. Aitken RJ, Best F, Richardson DW, Schats R, Simm G. Influence of caffeine on movement characteristics, fertilizing capacity and ability to penetrate cervical mucus of human spermatozoa J Reprod Fertil 1983;67: 19-27
  2. Aitken RJ, Mattei A, Irvine S. Paradoxical stimulation of human sperm motility by 2-deoxyadeno-sine. J Reprod Fertil 1986; 78: 515-27 https://doi.org/10.1530/jrf.0.0780515
  3. Aitken RJ, Kelly RW. Analysis of the direct effects of prostaglandins on human sperm function. J Reprod Fertil 1985; 73: 139-46 https://doi.org/10.1530/jrf.0.0730139
  4. Asch RH, Silber SJ. Microsurgical epididymal sperm aspiration and assisted reproductive techniques. Ann NY Acad Sci 1991;626: 101-10 https://doi.org/10.1111/j.1749-6632.1991.tb37904.x
  5. Cascieri M, Amann RP, Hammerstedt RH. Adenine nucleotide changes at initiation of bull sperm motility. T Biol Chem 1976; 251: 787
  6. Craft J, Bennett V, Nicholson N. Fertilizing ability of testicular spermatozoa. Lancet 1993;2: 342: 864
  7. Dohlman HG, Thorner J, Carson MG, Lefkowitz RJ. Model system for the study of seven-transmembrane segment receptors. Ann Rev Biochem 1991; 60: 653-88 https://doi.org/10.1146/annurev.bi.60.070191.003253
  8. Fakih H, MacLusky N, Dechemey A, Walliman T, Huszar G. Enhancement of human sperm motility and velocity in vitro: effects of calcium and creatine phosphate. Fertil Steril 1986; 46: 938-44 https://doi.org/10.1016/S0015-0282(16)49839-0
  9. Fink K, Zech H. Effect of incubation time in deep freezing human sperm. Wien Klin Wochenschr 1991;103:707-9
  10. Garbers DL, Kopf GS. The regulation of sperma-tozoa by calcium cyclic nucleotides. Adv Cyclic Nucleotide Res 1980; 13:251-306
  11. Gavella M, Lipovac Y. Pentoxifylline-mediated reduction of superoxide anion production by human spermatozoa. Andrologia 1992;24: 37-9 https://doi.org/10.1111/j.1439-0272.1992.tb02606.x
  12. Goltz JS, Gardner TK, Kanous KS, Lindemann CB. The interaction of pH and cAMP on activation of motility in Triton X-100 extracted bull sperm. Biol Reprod 1988;39: 1129 https://doi.org/10.1095/biolreprod39.5.1129
  13. Grizard G, Lombard-Vignon N, Boucher D. Changes in carnitine and acetyl carnitine in human semen during cryopreservation. Hum Reprod 1992; 7: 1245-8 https://doi.org/10.1093/oxfordjournals.humrep.a137835
  14. Hoskins DD, Casillas ER. The function of cyclic nucleotides in mammalian spermatozoa In Astwood EB, Greep RO. (eds) Handbook of Physiology Section 7 1975; Vol. 5. Williams, Wilkins, Baltimore, MD.
  15. Huszar G, Willets M, Corrales M. Hyaluronic acid (sperm selet) improves retention of sperm motility and velocity in normospermic and oligospermic specimens. Fertil Steril 1990; 54: 1127-34 https://doi.org/10.1016/S0015-0282(16)54016-3
  16. Kruger TF, Swanson RJ, Hamilton M, Simmons KF, Acosta AA, Matta JF, Oehniger S, Morshedi M. Abnormal sperm morphology and other semen parameters related to the outcome of the hamster oocyte human sperm penetration assay. Int J Androl 1988; 11: 107-13 https://doi.org/10.1111/j.1365-2605.1988.tb00986.x
  17. Lewis SE, Mooban JM, Thompson W. Effects of pentoxifylline on human sperm motility in normospemnic individuals using computer-assisted analysis. Fertil Steril 1993; 59: 418-23 https://doi.org/10.1016/S0015-0282(16)55708-2
  18. Makler A, Fisher M, Murillo O, Laufer N, Decherney A, Naftolin F. Factors affecting sperm motility. IX. survival of spermatozoa in various biological media and under different gaseous compositions. Fertil Steril 1984;41: 428-32
  19. Mendoza C, Tesarik J. Effect of follicular fluid on sperm movement characteristics. Fertil Steril 1990; 54: 1135-9 https://doi.org/10.1016/S0015-0282(16)54017-5
  20. Moohan JM, Winston RML, Lindsay KS. Variability of human sperm response to immediate and prolonged exposure to pentoxifylline. Hum Reprod 1993;8: 1696-700 https://doi.org/10.1093/oxfordjournals.humrep.a137916
  21. Moohan JM, Winston RML, Lindsay KS. The variable effects of 2-deoxyadenosine on hum sperm motility and hyperactivation in vitro. Hum Reprod 1995; 10: 1098-103 https://doi.org/10.1093/oxfordjournals.humrep.a136101
  22. Nagy ZP, Liu J, Joris H, Verheyen G, Tournaye H, Camus M, Derde MC, Devroey P, Van Steirteghem AC. The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod 1995; 10: 1123-9 https://doi.org/10.1093/oxfordjournals.humrep.a136104
  23. Nijs M, Vanderzwalmen P, Vandamme B, Segal-Bertin G, Lejeune B, Segal L, van Roosendaal E, Schoysman R. Fertilizing ability of immotile spermatozoa after intracytoplasmic sperm injection. Hum Reprod 1996; 11: 2180-5 https://doi.org/10.1093/oxfordjournals.humrep.a019073
  24. Palermo G, Joris H, Devroey P, Steirtegem AY. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340: 17-8 https://doi.org/10.1016/0140-6736(92)92425-F
  25. Schoenfeld CY, Amelar RD, Dubin L. Stimulation of ejaculated human spermatozoa by caffeine. Fertil Steril 1975; 26: 158-236 https://doi.org/10.1016/S0015-0282(16)40936-2
  26. Shen MR, Chiang PH, Yang RC. Pentoxifylline stimulates human sperm motility both in vitro and after oral therapy. Br J Clin Phannacol 1991; 31: 711-4 https://doi.org/10.1111/j.1365-2125.1991.tb05600.x
  27. Shrivastav P, Nadkami P, Wensvoort S, Craft I. Percutaneous epididymal sperm aspiration for obstructive azoospermia. Hum Reprod 1994;9: 2058-61 https://doi.org/10.1093/oxfordjournals.humrep.a138393
  28. Soldati G, Piffaretti-Yanez A, Campana A, Marchini M, Luerti M, Balerna M. Effect of peritoneal fluid on sperm motility and velocity distribution using objective measurements. Fertil Steril 1989; 52: 113-9 https://doi.org/10.1016/S0015-0282(16)60799-9
  29. Steirteghem AV, Nagy Z, Joris H, Lui J, Staessen C, Smitz J, Wisanto A, Devroey P. High fertilization and implantation rates after incytoplasmic sperm injection. Hum Reprod 1993; 8: 1061-6 https://doi.org/10.1093/oxfordjournals.humrep.a138192
  30. Storey BT. Energy metabolism of spermatozoa. IV. Effect of calcium on respiration of mature epididymal sperm of the rabbit. Biol Reprod 1975;12: 1 https://doi.org/10.1095/biolreprod12.1.1
  31. Tash JS, Means AR. Cyclic adenosine 3',5'mono-phosphate, calcium and protein phosphorylation in flagellar motility. Biol Reprod 1983;28: 75-104 https://doi.org/10.1095/biolreprod28.1.75
  32. Tash JS, Means AR. cAMP-dependent regulatory processes in the acquisition and control of sperm flagellar movement, in Meiotic inhibition: Molecular control of Meiosis, Halsetine, F. P. and First, N. L., Eds., Alan R. Liss, New York, 1988; 335
  33. Tesarik J, Thebault A, Testart J. Effect of pentoxifylline on sperm movement characteristics in nonno-zoospennic and asthenozoospennic specimens. Hum Reprod 1992;7: 1257-63 https://doi.org/10.1093/oxfordjournals.humrep.a137837
  34. Tournaye H, Janssens R, Devroey P, Van Steirteghem A. The influence of pentoxifylline on motility and viability of spermatozoa from normozoospemic semen samples. J Androl 1994a; 17: 1-8 https://doi.org/10.1111/j.1365-2605.1994.tb01200.x
  35. Ward A, Clissold SP. Pentoxifylline: a review of its pharmacodynamic and pharmacokinetic properties, and its therapeutic efficiency. Drugs 1987; 34: 50-60 https://doi.org/10.2165/00003495-198734010-00003
  36. Yanagimachi R. Fertility of mammalian spermatozoa: its development and relativity. Zygote 1994; 2: 371-2. Review https://doi.org/10.1017/S0967199400002240
  37. Yanagimachi R. Mammalian fertilization. In Knobil, E.,. NeilL, J.E., Ewing, L.L. et al., (eds), The Physiology of reproduction, Vol. 1- Raven Press, New York, 1988; 135-85
  38. Yovich JM, Edirisinghe WR, Cumniins JM, Yovich JL. Influence of pentoxifylline in severe male factor infertility. Fertil Steril 1990; 53: 715-22 https://doi.org/10.1016/S0015-0282(16)53470-0
  39. Yovich JM, Edirsinghe WR, Cummins JM, Yovich JL. Preliminary results using pentoxifylline in a pronuclear stage tubal transfer (PROST) program for severe male factor infertility. Fertil Steril 1988; 50: 179-81 https://doi.org/10.1016/S0015-0282(16)60030-4