• Title/Summary/Keyword: young seedlings

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Effect of Alternaria solani Exudates on Resistant and Susceptible Potato Cultivars from Two Different pathogen isolates

  • Shahbazi, Hadis;Aminian, Heshmatollah;Sahebani, Navazollah;Halterman, Dennis
    • The Plant Pathology Journal
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    • v.27 no.1
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    • pp.14-19
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    • 2011
  • Early blight of potato, caused by Alternaria solani, is a ubiquitous disease in many countries around the world. Our previous screening of several Iranian potato cultivars found that significant variation in resistance phenotypes exists between two cultivars: resistant 'Diamond' and susceptible 'Granula'. Our previous analysis of five different pathogen isolates also identified varying degrees of aggressiveness regardless of the host cultivar. Here, a bioassay was used to study the role of liquid culture exudates produced in vitro on pathogenicity and elicitation of disease symptomology in seedlings as well as detached leaves. Responses of host genotypes to the exudates of the two A. solani isolates were significantly different. Detached leaves of the resistant cultivar 'Diamond' elicited fewer symptoms to each isolate when compared to the susceptible cultivar 'Granula'. Interestingly, the phytotoxicity effect of the culture filtrate from the more aggressive isolate A was higher than from isolate N suggesting an increased concentration or strength of the toxins produced. Our results are significant because they indicate a correlation between symptoms elicited by A. solani phytotoxins and their aggressiveness on the host.

Growth regulation of cow1 rice mutant seedlings by blue light

  • Goh, Chang-Hyo;Ko, Suk-Min;Park, Hee-Yeon;Kim, Yeon-Ki;Kim, Yong-Woo;Kim, Young-Joo;Sun, Hyeon-Jin;Moon, Yong-Hwan;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • v.37 no.4
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    • pp.465-471
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    • 2010
  • We assessed whether the cow1 mutant defects are associated with growth of Tos17 and T-DNA insertional rice in blue light (BL). Growth of oscow1 mutants which encoded a member of the YUCCA protein family was retarded in BL. Root to shoot ratios of the mutants were reduced about 2 times lower in the absence of NAA and about 2.5 times lower in the presence of NAA; the shoot growth was not significantly changed by NAA addition. Photosynthetic activity of the mutants was however inhibited in high light. Pigment analysis showed significant difference between wild-type (Chl a:b = 3.02) and mutants (3.84). Carotenoid contents of the mutants were also decreased considerably, implying the involvement of cow1 in pigment formation. These findings lead us to suggest that the growth retardation of oscow1 mutant plants by BL results from the difference of photosynthetic activity in part.

A New Phalaenopsis Cultivar 'Jaha' with Large Purple Petal (적색대륜계 팔레놉시스 신품종 '자하' 육성)

  • Park, Young Su;Lim, Sung Hee;Lee, Sang Deok;Park, In Tae
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.3
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    • pp.205-207
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    • 2009
  • A new cultivar of Phalaenopsis 'Jaha' was developed at Cactus Research Institute, Gyeonggido A.R.E.S in 2006. A crossing between 'KYP74', a pink flower with red lip and 'KYP88', light pink colored flower in 1999 was obtained 264 seedlings. 'Jaha' was finally selected through the test of characteristics from 2002 to 2006. This cultivar has purple color flower with red purple lip, large flower, 11.0 flowers per plant, and 81.0 of inflorescence length. This new cultivar was registered for commercialization in 2006 and would be cultured under greenhouse condition in Korea.

A New Grafted Cactus(Gymnocalycium mihanovichii) Cultivar 'Yellow King' (접목선인장 비모란 'Yellow King'육성)

  • Cho, Chang Hui;Song, Cheon Young;Lee, Sang-Deok;Park, In-Tae
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.313-316
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    • 2008
  • A yellow grafted cactus of Gymnocalycium mihanovichii 'Yellow King' was bred by the Cactus Research Institute, Gyeonggi-do ARES. in 2007. A crossing between 'GG0214319' and 'GG021295' in 2005 was obtained 338 seedlings. 'Yellow King' was finally selected through the test of specific growth and glove coloring characteristics in 2007. The color of glove and tubercles is yellow. The shape of glove is round with 8~9 ribs. Spine is short and brown color. Growth is fast as 6 month after planting to reach 34.1 mm in diameter. Propagation ability is good to set as many as 12.4 tubercles.

A New Color Grafted Cactus (Copiapoa scopa) Cultivar "Red Stone" (접목선인장 코피아포아 신품종 "레드스톤" 육성)

  • Cho, Chang-Hui;Nam, Sang-Yong;Lee, Sang-Deok;Park, Young-Su
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.525-528
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    • 2008
  • A new graft cactus (Copiapoa scopa) cultivar 'Redstone' was developed at Cactus Research Institute, Gyeonggi-do A.R.E.S, in 2007. GCP563 and GCP618 were crossed in 2004 and we got 9 seedlings from this combination. After pedigree selection in 2005, one cultivar was finally selected through the test of specific character in 2007 and named as "Redstone". "Redstone" cultivar has red brown color and round globose shape with 15 ribs and its spines are black. This cultivar is able to produce 26.8 offshoots per plant. 'Redstone' cultivar has higher productivity and more slow growth than Copiapoa scopa cultivar.

Effects of Biomaterials Mixed with Artificial Soil on Seedling Quality of Fraxinus Rhynchophylla in a Containerized Production System

  • Dao, Huong Thi Thuy;Youn, Woo Bin;Han, Si Ho;Seo, Jeong Min;Aung, Aung;An, Ji Young;Park, Byung Bae
    • Journal of Forest and Environmental Science
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    • v.35 no.1
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    • pp.25-30
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    • 2019
  • The composition of artificial soil in a containerized seedling production plays an important role in seedling quality as well as environmental issues. We investigated the effects of different types of biomaterials and mixed ratio with artificial soil on the growth of Fraxinus rhynchophylla seedlings. Soil medium was supplemented with 3 levels (0%, 10%, 20%) of pine bark, mushroom sawdust and rice husk. Root collar diameter (RCD), height growth, and biomass have significantly increased when rice husk was applied. Compared with the control, RCD and height growth showed highest in 20% rice husk treatment with an increase of 5.7% and 17.6%, respectively. In contrast, the treatments of pine bark and mushroom sawdust showed lower results in growth parameters (RCD, height growth, and total biomass) than control. Seedling quality index was also highest at the 20% rice husk treatment, but there was not statistically different among treatments. Our results suggested rice husk can be substituted up to 20% of substrates for containerized F. rhynchophylla seedling production system.

First Report of Soft Rot by Pectobacterium carotovorum subsp. brasiliense on Amaranth in Korea

  • Jee, Samnyu;Choi, Jang-Gyu;Hong, Suyoung;Lee, Young-Gyu;Kwon, Min
    • Research in Plant Disease
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    • v.24 no.4
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    • pp.339-341
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    • 2018
  • Amaranth has the potential for good materials related to nutrients and health benefits. There are several diseases of amaranth such as leaf blight, damping-off, and root rot. As a causal agent of soft rot disease, Pectobacterium spp. could infect various plant species. In this study, we isolated the bacterial pathogen causing soft rot of amaranth in South Korea. In Gangneung, Gangwon province during 2017, amaranth plants showed typical soft rot symptoms such as wilting, defoliation and odd smell. To isolate pathogen, the macerated tissues of contaminated amaranth were spread onto LB agar plates and purified by a single colony subculture. One ml bacterial suspension of a representative isolate was injected to the stem of five seedlings of 2-week-old amaranth with a needle. Ten mM magnesium sulfate solution was used as a negative control. 16S rDNA gene and recA gene were sequenced and compared with the reference sequences using the BLAST. In the phylogenetic tree based on 16S rDNA gene and recA gene, GSA1 strain was grouped in Pcb.

Growth and Physiological Responses of Quercus acutissima Seedling under Drought Stress

  • Lim, Hyemin;Kang, Jun Won;Lee, Solji;Lee, Hyunseok;Lee, Wi Young
    • Plant Breeding and Biotechnology
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    • v.5 no.4
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    • pp.363-370
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    • 2017
  • In this study, Quercus acutissima seedlings were subjected to drought for 30 days then analyzed to determine their response to water deficit. The growth phenotype, chlorophyll fluorescence response, fresh weight, dry weight, photosynthetic pigment levels, soluble sugar content, and malondialdehyde (MDA) were measured to evaluate the effects of drought on plant growth and physiology. The growth phenotype was observed by infrared (IR) digital thermal imaging after 30 days of drought treatment. The maximum, average, and minimum temperatures of drought-treated plant leaves were $1-2^{\circ}C$ higher than those of the control. In contrast, the fresh and dry weights of the dehydrated leaves were generally lower than those of the control. There were no significant differences between treatments in terms of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid levels. Nevertheless, for the drought treatment, the $F_v/F_m$ and $F_v/F_o$ ratios (chlorophyll fluorescence response) were lower than those for the control. Therefore, photosynthetic activity was lower in the dehydrated plants than the control. The drought-stressed Q. acutissima S0536 had lower soluble sugar (glucose and fructose) and higher MDA levels than the controls. These findings may explain the early growth and physiological responses of Q. acutissima to dehydration and facilitate the selection of drought-resistant tree families.

E3 ligase BRUTUS Is a Negative Regulator for the Cellular Energy Level and the Expression of Energy Metabolism-Related Genes Encoded by Two Organellar Genomes in Leaf Tissues

  • Choi, Bongsoo;Hyeon, Do Young;Lee, Juhun;Long, Terri A.;Hwang, Daehee;Hwang, Inhwan
    • Molecules and Cells
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    • v.45 no.5
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    • pp.294-305
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    • 2022
  • E3 ligase BRUTUS (BTS), a putative iron sensor, is expressed in both root and shoot tissues in seedlings of Arabidopsis thaliana. The role of BTS in root tissues has been well established. However, its role in shoot tissues has been scarcely studied. Comparative transcriptome analysis with shoot and root tissues revealed that BTS is involved in regulating energy metabolism by modulating expression of mitochondrial and chloroplast genes in shoot tissues. Moreover, in shoot tissues of bts-1 plants, levels of ADP and ATP and the ratio of ADP/ATP were greatly increased with a concomitant decrease in levels of soluble sugar and starch. The decreased starch level in bts-1 shoot tissues was restored to the level of shoot tissues of wild-type plants upon vanadate treatment. Through this study, we expand the role of BTS to regulation of energy metabolism in the shoot in addition to its role of iron deficiency response in roots.

Characterization of Three Fusarium spp. Causing Wilt Disease of Cannabis sativa L. in Korea

  • Young Mo Koo;S. M. Ahsan;Hyong Woo Choi
    • Mycobiology
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    • v.51 no.3
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    • pp.186-194
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    • 2023
  • In July 2021, wilting symptoms were observed in adult and seedling hemp (Cannabis sativa L. cv. Cherry Blossom) plants grown in a greenhouse. As the disease progressed, yellowing and wilting symptoms on the leaves developed, resulting in whole plant death. In seedling plants, typical damping-off symptoms were observed. To identify the pathogen, the roots of diseased plants were sampled, surface sterilized, and cultured on potato dextrose agar (PDA) media. From the culture, 4 different fungal isolates were recovered and purely cultured. Each fungal isolate showed distinct growth shapes and color development on malt extract agar, oatmeal agar, sabouraud dextrose agar, and PDA media. Microscopic observation and molecular identification using ribosomal DNA internal transcribed spacer sequencing identified them as 3 Fusarium spp. and 1 Thielaviopsis paradoxa. Additional sequencing of elongation factor 1-alpha and b-tubulin regions of 3 Fusarium spp. revealed that 2 of them are Fusarium solani, and the other one is Fusarium proliferatum. To examine which isolate can act as a causal agent of wilt disease of hemp, each isolate was tested for their pathogenicity. In the pathogenicity test, F. solani AMCF1 and AMCF2, and F. proliferatum AMCF3, but not T. paradoxa AMCF4, were able to cause wilting disease in hemp seedlings. Therefore, we report that F. solani AMCF1 and AMCF2, and F. proliferatum AMCF3 as causal agents of Fusarium wilt of hemp plants. To our knowledge, this is the first report of the wilt disease of C. sativa L. caused by Fusarium spp. in Korea.