Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Soft Rot by Pectobacterium carotovorum subsp. brasiliense on Amaranth in Korea

  • Jee, Samnyu (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Jang-Gyu (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Hong, Suyoung (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Young-Gyu (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kwon, Min (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
  • Received : 2018.10.13
  • Accepted : 2018.12.06
  • Published : 2018.12.31
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Abstract

Amaranth has the potential for good materials related to nutrients and health benefits. There are several diseases of amaranth such as leaf blight, damping-off, and root rot. As a causal agent of soft rot disease, Pectobacterium spp. could infect various plant species. In this study, we isolated the bacterial pathogen causing soft rot of amaranth in South Korea. In Gangneung, Gangwon province during 2017, amaranth plants showed typical soft rot symptoms such as wilting, defoliation and odd smell. To isolate pathogen, the macerated tissues of contaminated amaranth were spread onto LB agar plates and purified by a single colony subculture. One ml bacterial suspension of a representative isolate was injected to the stem of five seedlings of 2-week-old amaranth with a needle. Ten mM magnesium sulfate solution was used as a negative control. 16S rDNA gene and recA gene were sequenced and compared with the reference sequences using the BLAST. In the phylogenetic tree based on 16S rDNA gene and recA gene, GSA1 strain was grouped in Pcb.

Keywords

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Fig. 1. Soft rot symptoms of amaranth caused by Pectobacterium carotovorum subsp. brasiliense on field (A, B), and artificial symptoms by inoculation (C). On third day after infection, infected plants showed soft rot symptoms and control was not affected by infection of 10 mM MgSO4.

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Fig. 2. Phylogenetic tree based on 16S rDNA sequences and recombinase A sequences of Pectobacterium strains. DNA sequences from the NCBI database were aligned using ClustalW and phylogenetic trees were constructed using the neighbor-joining method and visualized with MEGA7. Isolated bacteria is GSA1.

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