• 제목/요약/키워드: young seedlings

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Expression of Catalase (CAT) and Ascorbate Peroxidase (APX) in MuSI Transgenic Tobacco under Cadmium Stress

  • Kim, Kye-Hoon;Kim, Young-Nam;Lim, Ga-Hee;Lee, Mi-Na;Jung, Yoon-Hwa
    • 한국토양비료학회지
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    • 제44권1호
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    • pp.53-57
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    • 2011
  • The MuSI is known as a multiple stress resistant gene with several lines. A previous study using RT-PCR showed that the expression of MuSI gene in tobacco plant induced its tolerance to Cd stress. This study was conducted to examine the enhanced Cd tolerance of the MuSI transgenic tobacco plant through germination test and to understand the role of the involved antioxidant enzymes for the exhibited tolerance. Germination rate of MuSI transgenic tobacco was more than 10% higher than that of wild-type tobacco, and seedlings of MuSI transgenic tobacco grew up to 1.6 times larger and greener than seedlings of wild-type tobacco at 200 and 300 ${\mu}M$ Cd. From the third to the fifth day, CAT activities at 100 and 200 ${\mu}M$ Cd and APX activities at 100, 200 and 300 ${\mu}M$ Cd of MuSI transgenic tobacco were up to two times higher than those of wild-type tobacco. MuSI gene is shown to enhance the activities of antioxidant enzymes resulting in higher tolerance to oxidative stress compared with the control plant.

Measurement of ecological niche of Quercus aliena and Q. serrata under environmental factors treatments and its meaning to ecological distribution

  • Lee, Seung-Hyuk;You, Young-Han
    • Journal of Ecology and Environment
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    • 제35권3호
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    • pp.227-234
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    • 2012
  • Quercus aliena and Q. serrata are both occur as natural vegetation alongside natural freshwater bodies of the southern Korea Peninsula. Q. serrata dominates over Q. aliena as secondary forest vegetation in the present day. In order to explain these natural distributional traits of the oak species, we conducted some experiments with oak seedlings which treated with major important environmental resources, including light, moisture and nutrients, under controlled conditions. We then measured the ecological niche breadths and overlap from 15 eco-morphological characteristics. The ecological niche breadth of Q. aliena and Q. serrata were higher in terms of the nutrient factor applied, but was lower terms of light. The niche breadth of Q. serrata was wider than that of Q. aliena in light and moisture exposure. On the other hand, the niche breadth of Q. aliena was similar with that of Q. serrata in terms of the nutrient factor applied. These results imply that Q. serrata has a broader ecological distribution in over a wider variety of light and moisture environments than that of Q. alien. Ecological niche overlap between two oak species was the widest in terms of the light treatment factor applied, and narrowest in terms of moisture. This response pattern was also verified by cluster and principle component analysis. These results suggest competitive interactions between Q. serrata and Q. aliena seedlings may be higher for light resources than moisture or nutrient resources, and that Q. serrata is more shade tolerant than Q. aliena.

육묘기술수준별 경영성과 분석과 경영지도 방향 (The Effects of Management and Technical Capabilities in the Performance of Plug Seedling Production)

  • 김사균;이민수;최영찬
    • 농촌지도와개발
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    • 제12권1호
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    • pp.109-118
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    • 2005
  • This study was carried out to identify the major characteristics of plug seedling farm's management. Thirty glasshouse farms were surveyed out of 42 farms that adopted plug seedling production technology in Korea. The major objectives of this survey were to determine the technical capability of the glasshouse farmers, and to analyze the significant differences in terms of production performance and management capabilities. The major results of the survey were as follows : 1) The production quantity of plug seedlings of the superior group was 43% higher than the inferior group. 2) The plug seedling loss rate of the superior roup was lower by 4% than the inferior group. 3) The income of superior group was 4.2 million Won per $1,000m^2$, while the income of the inferior group was only 0.45 million Won. 4) The cause of low production of grafted plug seedlings was primarily due to the lack of technical knowledge and skills. 5) The results indicated that the technical knowledge level and production skills of grafting plug seedling should be improved.

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Screening methods for drought and salinity tolerance with transgenic rice seedlings

  • Song, Jae-Young;Song, Seon-Kyeong;Yu, Dal-A;Kim, Me-Sun;Kang, Kwon Kyoo;Cho, Yong-Gu
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.165-165
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    • 2017
  • Abiotic stress is one of the major serious limiting factors in rice (Oryza sativa) and caused rice production losses. It is important to precisely screen valuable genetic resources for improving stress tolerance and understanding tolerance mechanism to abiotic stresses. Because there are differences of experiment designs for screening of tolerant plant in several studies related to abiotic stress, this study has performed to provide the rapid and efficiency screening method for selection of tolerance rice to drought and salinity stresses. Two week-old rice seedlings that reached about three leaf stage were treated with drought and salinity stresses and examined tolerant levels with tolerant and susceptible control varieties, and transgenic plants. To determine the optimum concentration for the selection of drought and salinity condition, tolerant, susceptible and wild-type plants were grown under three soil moisture contents (5, 10 and 20% water contents) and three NaCl concentrations (100, 200 and 250 mM) for 10 days at seedling stage. 200 mM NaCl concentration and 5% moisture content soil were determined as the optimum conditions, respectively. The described methodologies in this study are simple and efficiency and might help the selection of drought and salinity tolerance plants at the 3,4-leaf-seedling stage.

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Tobacco Growth Promotion by the Entomopathogenic Fungus, Isaria javanica pf185

  • Lee, Yong-Seong;Kim, Young Cheol
    • Mycobiology
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    • 제47권1호
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    • pp.126-133
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    • 2019
  • Isaria javanica pf185 is an important entomopathogenic fungus with potential for use as an agricultural biocontrol agent. However, the effect of I. javanica pf185 on plant growth is unknown. Enhanced tobacco growth was observed when tobacco roots were exposed to spores, cultures, and fungal cell-free culture supernatants of this fungus. Tobacco seedlings were also exposed to the volatiles of I. javanica pf185 in vitro using I-plates in which the plant and fungus were growing in separate compartments connected only by air space. The length and weight of seedlings, content of leaf chlorophyll, and number of root branches were significantly increased by the fungal volatiles. Heptane, 3-hexanone, 2,4-dimethylhexane, and 2-nonanone were detected, by solid-phase micro-extraction and gas chromatography-mass spectrophotometry, as the key volatile compounds produced by I. javanica pf185. These findings illustrate that I. javanica pf185 can be used to promote plant growth, and also as a biocontrol agent of insect and plant diseases. Further studies are necessary to elucidate the mechanisms by which I. javanica pf185 promotes plant growth.

Effects of plant preservative mixtureTM on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its application in orchid conservation

  • Tran Trung Chanh;Nguyen Tan Huy;Nguyen Thu Ha;Khanh Le;Nguyen Huu Hoang
    • Journal of Plant Biotechnology
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    • 제50권
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    • pp.108-114
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    • 2023
  • In vitro conservation is one of the most effective strategies for rare plant protection, especially for orchid species. To maximize the success rates of in vitro explant establishment (stage I) in conservation programs, the application of tissue culture additives such as Plant Preservative MixtureTM (PPMTM) should be emphasized. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model for the evaluation of PPMTM's phytotoxicity in the meristematic tissues of epiphytic orchids. PPMTM had no observable inhibitory effect on protocorm, shoot, or root development when it was supplemented at 0.1%. PPMTM supplementation caused adverse effects on D. thyrsiflorum explants at concentrations > 0.2%. At high concentrations, young in vitro seedlings showed damage, especially at the root tissue level. Based on this model, supplementation of 0.1-0.2% PPMTM to culture media was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.