• Journal of Microbiology and Biotechnology
• /
• v.12 no.4
• /
• pp.617-621
• /
• 2002

In order to optimize the carrot hairy root culture for SOD production, a fed-batch culture of hairy roots was performed in a bioreactor. Maximum SOD activity was obtained when the hairy roots were transferred to the MS medium containing 110 g/1 concentration of sucrose. By controlling the sucrose concentration (70 g/1 sucrose for growth and 110 g/1 sucrose far production, respectively) In a two-stage fed-batch culture, 29 g/1 of the hairy roots was obtained based on the final dry mass. The volumetrically determined SOD activity and productivity in the fed-batch culture were about 6 times higher than those from the flask culture containing sucrose at 30 g/1 concentration.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.3
• /
• pp.560-567
• /
• 2008

An efficient method to produce water-soluble polysaccharides from Lentinus lepideus is described. The productivity of both endopolysaccharides (PPS) and exopolysaccharides (EPS) was compared under various culture conditions. The effect of treating their own PPS and EPS on immune cytokine production was also studied in relation to culture factors. High yield production of EPS required a moderate culture temperature $(25^{\circ}C)$ as well as long culture period (16-20 days). In contrast, PPS production required a high culture temperature $(30^{\circ}C)$ and short culture period (8 days). Most of the carbon sources did not affect polysaccharides and mycelial production except for sucrose. Immune cytokine levels in the EPS treatment varied among carbon sources or culture periods. PPS did not appear to affect much on the production of cytokines, regardless of the culturing factors, except for the culture period. These results suggest that the optimal culture conditions for L. lepideus vary according to culture purposes, and different culture conditions should be used for different targets including mycelial biomass, EPS, and PPS. Whereas the immunomodulating activitiy of EPS appeared to be affected by culture conditions in L. lepideus, that of PPS did not.

• Journal of Korean Academy of Nursing Administration
• /
• v.14 no.1
• /
• pp.5-12
• /
• 2008

Purpose: This study aimed to examine the impact of Organizational Culture on job satisfaction and organizational commitment in nursing organization. Methods: The sample consisted of 264 nurses of 2 tertiary medical institutions. The instruments used in this study were the nursing organizational culture questionnaire (Han, 2001), job satisfaction scale (Paula, 1978), and Organizational Commitment scale (Mowday et al., 1979). The data were analyzed by descriptive statistics, one-way ANOVA, t-test, Pearson Correlation. Coefficient, and multiple regression using the SPSS Win 14.0 program. Results: The dominant organizational culture of nursing organization was rank-oriented culture. The mean score of job satisfaction and organizational commitment was 3.28 and 3.40 respectively. Organizational commitment and Job satisfaction were positively correlated with affiliative-oriented culture, innovative-oriented culture, and task-oriented culture. Affiliative-oriented and task-oriented culture were major variables influencing on job satisfaction and affiliative-oriented culture was a major variable influencing organizational commitment. Conclusion: Organizational culture, especially affiliative-oriented culture had a significant influence on nurses' job satisfaction and organizational commitment. Therefore, we have to develop strategies to enhancing the affiliative-oriented culture to improve the job satisfaction and organizational commitment of nurses.

• Korea Journal of Hospital Management
• /
• v.15 no.2
• /
• pp.61-83
• /
• 2010

The purpose of this study is to analyze the effects of the introduction of 'business performance evaluation program' on the changes in organizational culture and employee commitment. The data for this study were collected three times, in 2006, 2008, and 2009 from the employees at a public hospital in Korea. The main findings of this study are as follows. 1. Organizational culture has gradually changed as the 'business performance evaluation program' was introduced and implemented. Regarding the changes in the different type of culture, we can see that group culture, developmental culture and rational culture has been stronger and, on the other hand, the introduction of 'the program' did not have as much impact on the hierarchical culture as on the other types of culture. 2. The group culture has have more impact on the organizational commitment than other types of culture. In case of the hierarchical culture, however, statistically significant relationships could not be found between the organizational commitment and the introduction of business performance evaluation program.

• Korea Journal of Hospital Management
• /
• v.14 no.3
• /
• pp.1-22
• /
• 2009

Purpose : The purpose of this study was to examine the effects of nursing organizational culture and head nurse's leadership on the job satisfaction. Methods : For the purpose, a structured questionnaire was conducted. The subjects were 232 nurses who were working in the 2 general hospitals. The data were collected from Sep. 1 to Sep. 20 of 2006. Data were analyzed using descriptive statistics, one-way ANOVA, t-test, Pearson Correlation Coefficient, and multiple regression. Results : The dominant organizational culture of nursing organization was rank-oriented culture. The mean score of head nurse's transformational leadership and transactional leadership was 3.20 and 2.79 respectively. The best group of job satisfaction was affiliative-oriented culture group(3.31). The score of job satisfaction of transformational leadership's group was higher than that of transactional leadership's group. Job satisfaction was positively correlated with affiliative-oriented culture, innovative-oriented culture, task-oriented culture, and transformational leadership. Concerning of the job satisfaction, affiliative-oriented culture, rotation time and age explained 25.7%. Conclusion : The organizational culture and head nurse's leadership was correlated with job satisfaction. Especially affiliative-oriented culture and task-oriented culture influenced the job satisfaction. Based on the results, further development should be continued to develop the effective head nurse's leadership and organizational culture to improve the job satisfaction of nurses.

• Journal of the Korean Society for Aviation and Aeronautics
• /
• v.29 no.1
• /
• pp.47-60
• /
• 2021

Just culture is an important factors in safety culture, and is related to the degree to which potential risk information is shared. Since the importance of the level of just culture has been highlighted in the aspect that it is related to the prevention of various safety accidents, research on just culture was required. In previous studies, there are attempts to develop a scale to measure the level of just culture and to reveal the degree of predicting the experience of accidents or errors and the actual reporting experience. However, It was not possible to clarify the factors that facilitate and inhibit the level of just culture for the formation of just culture. This study was conducted to help improve the level of the organization's just culture by specifically identifying factors that facilitate and inhibit the impact on the level of the just culture. As a result, it was possible to find the facilitating and inhibiting factors affecting the 6 factors of just culture.

• Proceedings of the Korean Institute of Interior Design Conference
• /
• 2006.11a
• /
• pp.177-178
• /
• 2006

This plan is for a different type of welfare facilities devised by analyzing problems with existing welfare centers and focusing on connection with residents and link between culture and welfare and among generations. In terms of re-development plan of Youngsun-2-dong, Youngdo-gu, Busan and the neighborhood under this plan, Youngsun-dong, Youngdo-gu is one of the densely populated districts of the aged, with lower growth rate and poor housing and welfare facilities. However, the district has a blessed natural environment and historical site of cultural relics of the Neolithic Age as well as growth potential as a logistics center with broader network of roads linking with Bukhang Bridge when Namhang Bridge construction is complete, showing the aspiration for change toward hi-tech maritime city in the 21st century of Youngdo-gu. This plan is expected to promote unity between the old and children, protection from social marginalization, and re-socialization education and set the stage for participation and solidarity of residents in the community.

• Reproductive and Developmental Biology
• /
• v.36 no.4
• /
• pp.291-294
• /
• 2012

Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2005.05a
• /
• pp.187.3-187
• /
• 2005

• Proceedings of the Korean Society of Community Living Science Conference
• /
• 2011.10a
• /
• pp.116-116
• /
• 2011