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Feeder Independent Culture of Mouse Embryonic Stem Cells  

Kim, Myoung Ok (School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University)
Ryoo, Zae Young (School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University)
Publication Information
Reproductive and Developmental Biology / v.36, no.4, 2012 , pp. 291-294 More about this Journal
Abstract
Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.
Keywords
ES cells; Differentiation; Feeder free culture;
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