• 원예과학기술지
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• 제31권4호
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• pp.467-472
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• 2013

국내 전역에서 수집된 야생 참외 계통 중 1986년에 20계통, 1990년에 16계통에 대해 재배시험결과는 계통간에 초장에 변이가 있었고 잎은 작은 편이었다. 성표현형은 양성웅화동주형 30계통, 자웅동주형 4계통, 양성화주형이 2계통이었다. 주지에서 암꽃착생은 양성화주형을 제외하고는 극히 드물었다. 양성웅화동주형과 자웅동주형은 측지의 제 1, 2절에는 암꽃착생이 현저하였다. 유과에는 모두 고미질이 확인되었다. 숙과의 색은 진황, 황, 연황, 황녹, 유백색이였으며 과중은 매우 작았고 숙과의 감미는 매우 낮았다. 일과당 종자수는 50.6-158.4개로 야생종 및 종족유지의 특징을 보여주고 있었다.

• The Plant Pathology Journal
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• 제32권5호
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• pp.377-387
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• 2016

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is one of the most important viruses of cultivated tomatoes worldwide, mainly causing yellowing and curling of leaves with stunting in plants. TYLCV causes severe problems in sub-tropical and tropical countries, as well as in Korea. However, the mechanism of TYLCV infection remains unclear, although the function of each viral component has been identified. TYLCV C4 codes for a small protein involved in various cellular functions, including symptom determination, gene silencing, viral movement, and induction of the plant defense response. In this study, through yeast-two hybrid screenings, we identified TYLCV C4-interacting host proteins from both healthy and symptom-exhibiting tomato tissues, to determine the role of TYLCV C4 proteins in the infection processes. Comparative analyses of 28 proteins from healthy tissues and 36 from infected tissues showing interactions with TYLCV C4 indicated that TYLCV C4 mainly interacts with host proteins involved in translation, ubiquitination, and plant defense, and most interacting proteins differed between the two tissues but belong to similar molecular functional categories. Four proteins-two ribosomal proteins, S-adenosyl-L-homocysteine hydrolase, and 14-3-3 family protein-were detected in both tissues. Furthermore, the identified proteins in symptom-exhibiting tissues showed greater involvement in plant defenses. Some are key regulators, such as receptor-like kinases and pathogenesis-related proteins, of plant defenses. Thus, TYLCV C4 may contribute to the suppression of host defense during TYLCV infection and be involved in ubiquitination for viral infection.

• 한국응용곤충학회지
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• 제43권2호
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• pp.103-109
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• 2004

경남 김해와 창원의 단감원에서 감관총채별레(Ponticulothrips diospyrosi Haga et Okajima)의 발생생태와 피해양상을 조사하였다. 황색끈끈이 트랩 조사(2002년)와 피해권엽 내부 정밀조사(2002,2003년)에 의하면 월동세대 성충은 4월 하순부터 5월 하순 사이에 월동처에서 단감원으로 이동하여 단감의 새 잎에 산란하며, 제 1세대 성충의 발생 최성기는 6월 상중순이었다. 피해권엽 정밀조사에 의한 제1세대 난, 약충, 번데기의 발생 최성기는 각각 5월 상중순, 5월 하순6월 상순, 6월 상순이었다. 대부분의 제1세대 성충은 우화후 월동처로 이동하지만 일부 성충이 산란한 개체가 발육하여 제2세대 성충이 되는데, 7월 하순 이후에 발생하는 성충이 제2세대 성충인 것으로 생각된다. 피해엽율은 시기가 경과할수록 증가하였으며 창원포장에서 6월 상순에 9.7%로 가장 높았다. 피해과율은 6월 중순 낙화 이후부터 관찰되었으며 김해 B포장에서 9월 상순의 피해과율이 30.2%로 가장 높았다. 과수원의 가장자리에 가까이 위치한 나무일수록 피해엽 발생시기가 빨랐으며, 특히 소나무 군락을 중심으로 l0m이내에 피해가 심하였다. 성충의 발생시기와 관련하여 방제시기 문제를 고찰하였다.

• 한국식품조리과학회지
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• 제18권2호
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• pp.164-172
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• 2002

멥쌀가루에 첨가되는 솔잎가루의 양, 당의 종류와 양, 물의 양의 차이가 솔설기의 관능적 특성과 텍스쳐에 미치는 영향을 실험한 결과를 요약하면 다음과 같다. 1. 관능평가 결과, 색과 향기는 멥쌀가루 350g에 솔잎가루 10.5g(3%), 설탕 60g을 첨가한 군(A03S)이 유의하게 가장 좋다고 평가되었고(p〈0.05), 거친정도, 촉촉한정도, 쫄깃한정도는 멥쌀가루 350g에 솔잎가루 3.5g(1%), 꿀 80m1를 첨가한 군(A01H)이 유의하게 가장 높게 평가되었고(p〈0.05), 단정도는 멥쌀가루 350g에 솔잎가루 7g(2%), 설탕 60g을 첨가한 군(A02S)이 유의하게 가장 달다고 평가되었다(P<0.05). 바람직한 정도는 멥쌀가루 350g에 솔잎가루 3.5g(1%), 꿀 80m1를 첨가한 군(A01H)이 유의하게 가장 좋다고 평가되었다(p〈0.05). 솔잎가루 1, 2, 3% 첨가한 순으로 좋다고 평가되었으며, 선호도가 높게 평가된 솔설기가 grain이 곱고, moistness가 촉촉하고, chewiness가 쫄깃거렸다. 2. 기계적검사 결과 탄력성, 응집성, 견고성은 멥쌀가루 350g에 솔잎가루 3.5g(1%), 설탕 60g을 첨가한 군(A01S)이 유의하게 가장 높았고(p〈0.05), 씹힘성은 멥쌀가루 350g에 솔잎가루 7g(2%), 올리고당 90ml를 첨가한 군(A02O)이 유의하게 가장 높았고(P<0.05), 점착성은 멥쌀가루 350g에 솔잎가루 3.5g(1%), 올리고당 90m1를 첨가한 군(A01O)이 유의하게 가장 높았고(p〈0.05), 부착성은 멥쌀가루 350g에 솔잎가루 7g(2%), 꿀 80ml를 첨가한 군(A02H)이 유의하게 가장 높았다(P<0.05). 3. 수분함량은 멥쌀가루 33.2%, 솔잎가루 2.6% 였고 37∼40% 정도라야 바람직한 솔설기라고 평가되었다. 4. 색도측정 결과 L값(Lightness)은 멥쌀가루 350g에 솔잎가루 3.5g(1%), 꿀 80ml를 첨가한 군(A01H)이 가장 밝았으며, a값(Redness)과 b값(Yellowness) 은 멥쌀가루 350g에 솔잎가루 10.5g(3%), 설탕 60g을 첨가한 군(AO3S)이 가장 높아 red와 yellow에 가까웠다(p〈0.05). 이상의 연구를 통해 얻은 솔설기의 가장 바람직한 배합비는 멥쌀가루 350g에 솔잎가루 3.5g, 꿀 80m1, 소금 3.5g, 물 20m1를 넣고 찐 떡이었으며, 수분함량은 37.1% 였다. 솔잎은 약재로 여러 질병의 치료나 구황식푼으로 많이 상용해 왔는데, 솔잎을 떡에 첨가하여 음식으로 섭취할 수 있는 솔설기를 개발함으로서 일상적인 기능성 음식으로 솔설기가 널리 보급되었으면 하고 기대한다.

• 한국산림과학회지
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• 제49권1호
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• pp.32-33
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• 1980

1980년(年) 9월(月), 수원(水原) 임목육종연구소(林木育種硏究所)의 포플러묘포(苗圃)에 식재(植栽)되어 있는 Populus deltoides 클론중(中)에서 잎에 모자이크병징(病徵)을 나타내는 개체(個体)가 수본(數本) 관찰(觀察)되었다. 모자이크병(病) 반조직(班組織)의 즙액(汁液)을 dip법(法)에 의(依)해 2% 인산(燐酸)텅스텐으로 Negative염색(染色)하여 전자현미경(電子顯微鏡)으로 검경(検鏡)한 결과(結果), 외국(外國)에서 포플러 모자이크 바이러스로 보고(報告)된 것과 같은 크기를 가진 길이 660~670nm되는 바이러스잎자(粒子)가 다수(多數) 관찰(觀察)되었다.

• 한국식품영양학회지
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• 제29권6호
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• pp.1015-1022
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• 2016

The purpose of this study was to investigate ash and mineral contents of agricultural products that are widely and specifically grown in Korea, including 23 fruits, 11 cereals and specialty crops, and 11 leaves and vegetables. Pre-treatment of mineral was performed by microwave method. Mineral contents were analyzed by Inductively Coupled Plasma-Optical Emission Spectrometer (ICP-OES) and Inductively Coupled Plasma Spectrophotometry Mass (ICP-MS). Ash contents ranged from 0.20 to 0.69 g/100 g in fruits, 1.62 to 3.80 g/100 g in cereals, and 0.28 to 2.93 g/100 g in leaves and vegetables. Among the 45 samples, the highest contents of ash were found in quinoa (average 3.80 g/100 g), the highest contents of calcium (Ca) were found in Hansan ramie leaves (average 894.79 mg/100 g), the highest contents of phosphorus (P) were found in yellow amaranth(average 661.88 mg/100 g), the highest contents of potassium (K) were found in quinoa (average 1,455.38 mg/100 g), the highest contents of magnesium (Mg) were found in red amaranth (average 434.02 mg/100 g), the highest contents of molybdenum (Mo) were found in moringa (average $482.50{\mu}g/100g$), and the highest contents of selenium (Se) were found in apple mango(average $23.67{\mu}g/100g$).

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.23-23
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• 2017

Rice (Oryza sativa L.) is the world's most important cereal crop. In crop plant, chlorophyll content and leaf senescence could affect grain filling and yield. We analyzed a QTL associated with chlorophyll content and delayed leaf senescence using high chlorophyll near isogenic line (HC-NIL). HC-NIL derived from a cross between Oryza sativa cv. Hwaseong as a recurrent parent and wild species O. grandiglumis as a donor parent showed higher chlorophyll content than Hwaseong. To identify QTL associated with chlorophyll content, 58 $F_3$ and 38 $F_4$ lines were developed from a cross between HC-NIL and Hwaseong. For QTL analysis, simple sequence repeat (SSR) markers were used for genotyping and one-way ANOVA was conducted. A QTL for chlorophyll content (qCC2) was detected in chromosome 2 and explained 24.63% of phenotypic variation. The senescence effect of the qCC2 was examined in dark-induced incubation (DII). Detached leaves from Hwaseong and HC-NIL were incubated on 3mM MES buffer (pH 5.8) at $27^{\circ}C$ under complete dark condition. After 3 days of incubation, the Hwaseong leaves turned yellow, but the HC-NIL leaves were green. HC-NIL has higher chlorophyll content with delayed senescence than Hwaseong. These results indicated that qCC2 is associated with stay-green phenotype. To know whether the qCC2 is responsible for leaf functionality, ion leakage test and Fv/Fm measurement were performed. Both experiment results showed that differences were observed between Hwaseong and HC-NIL but it was not statistically significant. These results might suggest that the qCC2 is possibly related to chlorophyll content and non-functional stay-green phenotype.

• The Plant Pathology Journal
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• 제21권3호
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• pp.262-269
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• 2005

An antiviral producing bacterial strain was isolated from a ginseng rhizosphere in Kangwon province of Republic of Korea. In order to identify the bacterial strain, microbiological, physiological and biochemical tests were performed, along with RAPD, 16S rRNA, 16S-23S rRNA ITS (intergenic spacer region) and DNA-DNA hybridization analyses. The bacterium was found to be a strain of Pseudomonas fluorescens, which was designated as Gpf01. The strain was grown in Muller-Hinton (MH) broth, and the culture supernatant obtained was filtered through a $0.45{\mu}l$ filter. It was further boiled at $100^{\circ}C$ and tested in two experiments for its ability to control a yellow strain of Cucumber mosaic virus (CMV-Y). In the first experiment, boiled culture filtrate (RCF) was treated on one half of the leaves of Chenopodium amaranticolor followed by CMV- Y inoculation on both halves. In the second experiment, BCF was treated on the lower leaves of Nicotiana tobacum var. Xanthi-nc, with the CMV-Y mechanically inoculated onto the upper untreated leaves. In the first experiment, BCF treatment was able to considerably reduce the number of viral lesion, and in the second experiment, plants treated with BCF showed no visible viral symptoms compared to the Muller-Hinton (MH) media treated controls 15 days post inoculation (dpi), and remained symptomless throughout the study period. Thus, Gpf01, identified as P. fluorescence, was able to produce an antiviral component in the culture filtrate, which was found to be heat stable, non-phytotoxic and effective in local as well as systemic hosts of CMV.

• 식물병연구
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• 제10권2호
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• pp.126-129
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• 2004

2002년 7월 전북지방에 야생하는 칡의 잎에 점무늬병이 발병하였다. 잎에 처음에는 작은 점무늬증상이 나타나고. 이 점무늬 주변에 황색의 halo가 형성되는 것이 전형적인 특징이었다. 병반들이 점차 확대되어 융합되어 잎 전체가 황색으로 변색되며 결국 낙엽이 된다. 병원균이 분리되었으며 본 병원균의 균사집락은 PDA 배지에서 밝은 회녹색에서 암갈색이었다. 분생포자는 대부분 단생이며 드물게 3-8개 포자가 연쇄상으로 형성되었다. 분생포자는 암갈색이며 긴 난형 또는 곤봉형이고 크기는 20-60${\times}$10-25 $\mu\textrm{m}$이었다. 격막은 4-8개의 횡격막과 1-2개의 종격막을 형성하였다. 칡의 잎에 발생한 병징과 본 병원균의 균학적 특징을 검정한 결과, 이 병을 Alternaria tenuissima에 의한 칡 점무늬병으로 명명하고자 제안한다.

• The Plant Pathology Journal
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• 제29권2호
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• pp.193-200
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• 2013

Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 weeks post inoculation (WPI). CMV titre in Arabidopsis leaves was determined by indirect enzyme-linked immunosorbent assay (ELISA) at 2 WPI. Our results demonstrated that among all Arabidopsis plants treated with barley grain inoculum (BGI) of SKT-1 NahG and npr1 plants showed no significant reduction in disease severity and CMV titre as compared with control plants. In contrast, disease severity and CMV titre were significantly reduced in all Arabidopsis plants treated with culture filtrate (CF) of SKT-1 as compared with control plants. RT-PCR results showed increased expression levels of SA-inducible genes, but not JA/ET-inducible genes, in leaves of BGI treated plants. Moreover, expression levels of SA- and JA/ET-inducible genes were increased in leaves of CF treated plants. In conclusion, BGI treatment induced systemic resistance against CMV through SA signaling cascade in Arabidopsis plants. While, treatment with CF of SKT-1 mediated the expression of a majority of the various pathogen related genes, which led to the increased defense mechanism against CMV infection.