• The Plant Pathology Journal
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• 제37권2호
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• pp.173-181
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• 2021

The genus Streptomyces demonstrates enormous promise in promoting plant growth and protecting plants against various pathogens. Single and consortium treatments of two selected Streptomyces strains (Streptomyces shenzhenensis TKSC3 and Streptomyces sp. SS8) were evaluated for their growth-promoting potential on rice, and biocontrol efficiency through induced systemic resistance (ISR) mediation against Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent of rice bacterial leaf streak (BLS) disease. Seed bacterization by Streptomyces strains improved seed germination and vigor, relative to the untreated seed. Under greenhouse conditions, seed bacterization with consortium treatment TKSC3 + SS8 increased seed germination, root length, and dry weight by 20%, 23%, and 33%, respectively. Single and consortium Streptomyces treatments also successfully suppressed Xoc infection. The result was consistent with defense-related enzyme quantification wherein single and consortium Streptomyces treatments increased peroxidase (POX), polyphenol oxidase, phenylalanine ammonia-lyase, and β,1-3 glucanase (GLU) accumulation compared to untreated plant. Within all Streptomyces treatments, consortium treatment TKSC3 + SS8 showed the highest disease suppression efficiency (81.02%) and the lowest area under the disease progress curve value (95.79), making it the best to control BLS disease. Consortium treatment TKSC3 + SS8 induced the highest POX and GLU enzyme activities at 114.32 µmol/min/mg protein and 260.32 abs/min/mg protein, respectively, with both enzymes responsible for plant cell wall reinforcement and resistant interaction. Our results revealed that in addition to promoting plant growth, these Streptomyces strains also mediated ISR in rice plants, thereby, ensuring protection from BLS disease.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.67.2-68
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• 2003

A rice cultivar, Taebaegbyeo, is highly resistant to rice blast and moderately resistant to bacterial leaf blight (BLB) caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. To study the rice disease resistance mechanism, we generated rice deletion M3 mutants by gamma-ray irradiation. Blast and BLB responses of 16,000 M3 mutants were screened by inoculating mixtures of 4 races (KJ-201, H-1113a, KI-313, KI-409) of M. grisea and 3 Korean races of X. oryzae pv. oryzae. We selected so far 21 M3 mutants of Taebaegbyeo showing high susceptibility to the diseases. One of the mutants, KCT-6417, was susceptible to KI-1113a race of M. grisea, suggesting the deletion of a race-specific blast resistance gene in the mutant. To isolate rice genes involved in blast resistance and defense response, we take a PCR-based suppression subtractive hybridization approach using cDNAs of blast-inoculated wild type and the KCT-6417 as a tester and a driver, respectively. Genes specifically expressed in the wild type will be presented. The selected genes would give us a clue to understand mechanism for the race specific resistance and defense responses against M. grisea H-1113a in Taebaegbyeo.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.166-166
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• 2017

OsTAT encodes a twin-arginine translocator (TAT) pathway signal protein. It contains a TRANS membrane domain and a chloroplast transit peptide. mRNA transcription profiling of OsTAT1 revealed that it is highly overexpressed in the leaves corroborating reports on its role in chloroplast. Moreover, its level of expression is more pronounced during earlier stages (germination, 3-leaf stage, and maximum tillering) of growth in rice. A lower disease progress curve of bacterial blight is evident in transgenic lines compared with the wild type, Dongjin indicating its involvement in immunity to Xoo. Expression pattern following infection of Xoo strain K2 depicts highest levels at 4 and 8 hour post-inoculation which implies crucial induction of resistance during early response. This study initially reports a new overview on the biological functions of plant's TAT pathway. Further molecular and genetic analyses are underway to provide detailed involvement of OsTAT in disease resistance.

• 식물병연구
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• 제20권3호
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• pp.189-195
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• 2014

Genetic engineering is being used to enhance disease resistance and nutritional value of crops including rice plant. Considering the fast-growing agricultural biotechnology and rapidly increasing global area of transgenic crops, the risk evaluation on environment is necessary. In this study, we surveyed the difference of disease occurrence between transgenic rice variety, Iksan526 transformed with peanut stilbene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong in the field. Moreover, the possibility of gene transfer from transgenic rice to bacterial and fungal pathogens was investigated. The results of this study indicated that there was no significant difference in the occurrence and severity of the diseases between Iksan526 and Dongjin or Nampyeong. In addition, the results suggested that rice pathogen, such as Xanthomonas oryzae pv. oryzae, Rhizoctonia solani and Magnaporthe grisea did not take up stilbene synthase and bar genes under natural conditions. Moreover the transformed DNA was not transferred to the pathogens even in repetitive contacts.

• Mycobiology
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• 제43권3호
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• pp.311-318
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• 2015

Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding ${\beta}$-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1265-1274
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• 2015

Secondary metabolite-based chemotaxonomic classification of Streptomyces (8 species, 14 strains) was performed using ultraperformance liquid chromatography-quadrupole-time-offlight-mass spectrometry with multivariate statistical analysis. Most strains were generally well separated by grouping under each species. In particular, S. rimosus was discriminated from the remaining sevens pecies (S. coelicolor, S. griseus, S. indigoferus, S. peucetius, S. rubrolavendulae, S. scabiei, and S. virginiae) in partial least squares discriminant analysis, and oxytetracycline and rimocidin were identified as S. rimosus-specific metabolites. S. rimosus also showed high antibacterial activity against Xanthomonas oryzae pv. oryzae, the pathogen responsible for rice bacterial blight. This study demonstrated that metabolite-based chemotaxonomic classification is an effective tool for distinguishing Streptomyces spp. and for determining their species-specific metabolites.

• 한국육종학회지
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• 제43권4호
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• pp.241-251
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• 2011

Bacterial blight(BB) caused by Xanthomonas oryzae pv. oryzae(Xoo) is one of the most economically destructive bacterial diseases of rice in worldwide. Utilization of resistant cultivars carrying resistant gene(s) is relatively an effect method to control this disease. About 34 resistant genes for BB resistance have been identified in many countries. Among them, Xa1 and Xa3 genes against bacterial blight have been incorporated into improved korean japonica rice varieties. Now, Ilmi carrying Xa1 gene and severial cultivars carrying Xa3 gene are widely grown in our country. In recent year, xa5, Xa21 and Xa23 genes are using in rice breeding programs for japonica resistant cultivars to bacterial blight. Resistant cultivars incorporated with a diverse single gene and two, three, or the more major gene necessite in the future.

• 농업과학연구
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• 제16권1호
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• pp.36-43
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• 1989

Streptomyces의 분류, 분포 및 항생물질 생산특성을 알아보고자 각 토양으로부터 총 826 균주의 Streptomyces를 분리하여 토양별 분포를 조사하고 항생물질 생산능을 검정하였다. 토양별 방선균류의 분포를 조사한 결과 참나무, 잔디, 아카시아, 사과나무, 고추밭, 배추밭, banana 온실 등지에서 $10^{-5}g$ 토양중에 5개 이상의 Streptomyces가 분리되었으며 산림토양에서 평균 4.7개, 과수원 토양에서 2.4개, 경작지 토양에서 2.9개의 분리율을 보였다. 2. Streptomyces의 항균 spectrum을 조사한 결과 Bacillus subtilis, Erwinia carotovora sub-sp. carotovora, Xanthomonas campestis pv. oryzae에 49% 이상의 균주가 항균력을 나타내었으나 Escherichia coli와 Pseudomonas solanacearum에는 항균력을 나타내는 균주가 적었다. 3. 도열병균을 대상으로 Streptomyces의 항진균성 항생물질 생성능을 조사한 결과 강력한 항균력을 나타내는 균주가 40 균주였다. 4. Streptomyces의 분리율이 가장 우수한 배지는 Starch agar 였다. 5. Streptomyces의 항생물질 생성능이 가장 우수한 배지는 potato sucrose agar 였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.49-49
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• 2020

Bacterial leaf blight (BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a total of 10,000 accessions of rice germplasm were tested to resistance degree of four Korean isolated races (K1, K2, K3 and K3a) of Xoo by bioassay and a diverse 268 accessions was selected to the genome-wide association study (GWAS) using high quality 34,724 SNPs to identify the associated with resistance loci. LOC_Os04g53160 of chromosome 4 was significantly associated with K1 race resistant. LOC_Os11g46230 and LOC_Os11g47150 of chromosome 11 were highly associated with K2 and K3 races as 23.7 and 27.4 of -log(P) value, but K3a resistant loci was weakly associated at LOC_Os03g55270 of chromosome 3. The results of the GWAS validate known gene of BLB resistant and identified novel loci of R genes that provide useful targets for further investigation to help the breeding system and identified gene and QTL provide valuable sources for further functional characterization.

• 한국응용곤충학회지
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• 제24권2호
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• pp.97-101
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• 1985

한국(韓國)에서 발생(發生)하고 있는 흰잎마름병균의 병원성(病原性) 변이연구(變異硏究)는 일본판별품종(日本判別品種)에 의한 일본(日本) 분류체계(分類體系)를 이용(利用)하여 전국적(全國的)인 균형(菌型)의 분포상태(分布狀態)를 조사(調査)하여 왔으나 일본판별품종(日本判別品種)을 사용(使用)한 균형분류(菌型分類) 결과(結果) 동일(同一) 균군중(菌群中)에서도 다수계품종(多收系品種)에 따라 상이(相異)한 반응(反應)이 나타나 일본판별품종(日本判別品種)을 이용(利用)한 균형분류(菌型分類)가 부적합(不適合)한 것으로 나타났고, 일본판별품종(日本判別品種)을 한국(韓國)에서 증식(增殖) 보존(保存)할때 출수(出穗)가 늦거나 도복(倒伏)에 약(弱)하여 채종(採種)하기 어려운 점이 있으므로 한국(韓國)에 알맞는 판별품종(判別品種)을 선발(選拔)하기 위하여 한국품종중(韓國品種中)에서 선발(選拔)된 밀양(密陽) 23호(號), 청청(靑靑)벼 등(等) 7품종(品種)과 일본판별품종(日本判別品種)을 각(各) 균주별(菌株別)로 접종(接種) 비교(比較)한 결과(結果) 우리나라 흰잎마름병균에 대(對)한 저항성(抵抗性)과 이병성반응(罹病性反應)이 뚜렷하고 저항성유전자(抵抗性遺傳子)가 다르다고 추정(推定)되는 품종중(品種中)에서 밀양(密陽) 42호(號), 한강(漢江)찰벼, 풍산(豊産)벼, 청청(靑靑)벼, 밀양(密陽) 23호(號)가 한국(韓國) 흰잎마름병균의 병원성(病原性) 변이판별(變異判別)에 적합(適合)한 판별품종(判別品種)으로 선발(選拔)되었다. 우리나라 흰잎마름병 병원균(病原菌)의 병원성(病原性)은 일본판별품종(日本判別品種)의 반응(反應)에 의하여 I, II, III, IV, V 균군(菌群) 등(等)으로 분류(分類)되어 왔으나 한국판별품종(韓國判別品種)의 반응(反應)에 따라 Kl, K2, K3, K4, K5 레이스로 명명(命名)하였다.