Browse > Article
http://dx.doi.org/10.5423/PPJ.OA.05.2020.0083

Induction of Systemic Resistance against Bacterial Leaf Streak Disease and Growth Promotion in Rice Plant by Streptomyces shenzhenesis TKSC3 and Streptomyces sp. SS8  

Hata, Erneeza Mohd (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia)
Yusof, Mohd Termizi (Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia)
Zulperi, Dzarifah (Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia)
Publication Information
The Plant Pathology Journal / v.37, no.2, 2021 , pp. 173-181 More about this Journal
Abstract
The genus Streptomyces demonstrates enormous promise in promoting plant growth and protecting plants against various pathogens. Single and consortium treatments of two selected Streptomyces strains (Streptomyces shenzhenensis TKSC3 and Streptomyces sp. SS8) were evaluated for their growth-promoting potential on rice, and biocontrol efficiency through induced systemic resistance (ISR) mediation against Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent of rice bacterial leaf streak (BLS) disease. Seed bacterization by Streptomyces strains improved seed germination and vigor, relative to the untreated seed. Under greenhouse conditions, seed bacterization with consortium treatment TKSC3 + SS8 increased seed germination, root length, and dry weight by 20%, 23%, and 33%, respectively. Single and consortium Streptomyces treatments also successfully suppressed Xoc infection. The result was consistent with defense-related enzyme quantification wherein single and consortium Streptomyces treatments increased peroxidase (POX), polyphenol oxidase, phenylalanine ammonia-lyase, and β,1-3 glucanase (GLU) accumulation compared to untreated plant. Within all Streptomyces treatments, consortium treatment TKSC3 + SS8 showed the highest disease suppression efficiency (81.02%) and the lowest area under the disease progress curve value (95.79), making it the best to control BLS disease. Consortium treatment TKSC3 + SS8 induced the highest POX and GLU enzyme activities at 114.32 µmol/min/mg protein and 260.32 abs/min/mg protein, respectively, with both enzymes responsible for plant cell wall reinforcement and resistant interaction. Our results revealed that in addition to promoting plant growth, these Streptomyces strains also mediated ISR in rice plants, thereby, ensuring protection from BLS disease.
Keywords
biocontrol agent; plant growth-promoting agent; Streptomyces; Xanthomonas oryzae pv. oryzicola;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Khan, M. A., Naeem, M. and Iqbal, M. 2014. Breeding approaches for bacterial leaf blight resistance in rice (Oryza sativa L.), current status and future directions. Eur. J. Plant Pathol. 139:27-37.   DOI
2 Kurth, F., Mailander, S., Bonn, M., Feldhahn, L., Herrmann, S., Grobe, I., Buscot, F., Schrey, S. D. and Tarkka, M. T. 2014. Streptomyces-induced resistance against oak powdery mildew involves host plant responses in defense, photosynthesis, and secondary metabolism pathways. Mol. Plant-Microbe Interact. 27:891-900.   DOI
3 Lee, H.-J., Park, K.-H., Shim, J.-H., Park, R.-D., Kim, Y.-W., Cho, J.-Y., Hwangbo, H., Kim, Y.-C., Cha, G.-S. Krishnan, H.-B. and Kim, K.-Y. 2005. Quantitative changes of plant defense enzymes in biocontrol of pepper (Capsicium annuum L.) late blight by antagonistic Bacillus subtilis HJ927. J. Microbiol. Biotechnol. 15:1073-1079.
4 Lever, M. 1972. A new reaction for colorimetric determination of carbohydrates. Anal. Biochem. 47:273-279.   DOI
5 Liu, W., Liu, J., Triplet, L., Leach, J. E. and Wang, G.-L. 2014. Novel insights into rice innate immunity against bacterial and fungal pathogens. Annu. Rev. Phytopathol. 52:213-241.   DOI
6 Nino-Liu, D. O., Ronald, P. C. and Bogdanove, A. J. 2006. Xanthomonas oryzae pathovars: model pathogens of a model crop. Mol. Plant Pathol. 7:303-324.   DOI
7 Olanrewaju, O. S. and Babalola, O. O. 2019. Streptomyces: implications and interactions in plant growth promotion. Appl. Microbiol. Biotechnol. 103:1179-1188.   DOI
8 Oluwambe, T. M. and Kofoworola, A. A. 2016. Comparison of single culture and the consortium of growth-promoting rhizobacteria from three tomato (Lycopersicon esculentum Mill) varieties. Adv. Plants Agric. Res. 5:448-455.
9 Ouhdouch, Y., Barakate, M. and Finance, C. 2001. Actinomycetes of Moroccan habitats: isolation and screening for antifungal activities. Eur. J. Soil Biol. 37:69-74.   DOI
10 Pal, K. K. and Gardener, B. M. 2006. Biological control of plant pathogens. URL https://www.apsnet.org/edcenter/disimpact-mngmnt/topc/Documents/PHI-BiologicalControl.pdf [14 January 2019].
11 Rais, A., Jabeen, Z., Shair, F., Hafeez, F. Y. and Hassan, M. N. 2017. Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLoS ONE 12:e0187412.   DOI
12 Pieterse, C. M. J., Zamioudis, C., Berendsen, R. L., Weller, D. M., Van Wees, S. C. M. and Bakker, P. A. H. M. 2014. Induced systemic resistance by beneficial microbes. Annu. Rev. Phytopathol. 52:347-375.   DOI
13 Poulin, L., Raveloson, H., Sester, M., Raboin, L.-M., Silue, D., Koebnik, R. and Szurek, B. 2014. Confirmation of bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola on rice in Madagascar. Plant Dis. 98:1423.
14 Rahman, A., Hosokawa, S., Oono, Y., Amakawa, T., Goto, N. and Tsurumi, S. 2002. Auxin and ethylene response interactions during arabidopsis root hair development dissected by auxin influx modulators. Plant Physiol. 130:1908-1917.   DOI
15 Sharma, M., Dangi, P. and Choudhary, M. 2014. Actinomycetes: source, identification, and their applications. Int. J. Curr. Microbiol. Appl. Sci. 3:801-832.
16 Viaene, T., Langendries, S., Beirinckx, S., Maes, M. and Goormachtig, S. 2016. Streptomyces as a plant's best friend? FEMS Microbiol. Ecol. 92:fiw119.   DOI
17 Singh, R. A. and Rao, M. H. S. 1977. A simple technique for detecting Xanthomonas oryzae in rice seeds. Seed Sci. Technol. 5:123-127.
18 Sreevidya, M., Gopalakrishnan, S., Kudapa, H. and Varshney, R. K. 2016. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Braz. J. Microbiol. 47:85-95.   DOI
19 Suarez-Moreno, Z. R., Vinchira-Villarraga, D. M., Vergara-Morales, D. I., Castellanos, L., Ramos, F. A., Guarnaccia, C., Degrassi, G., Venturi, V. and Moreno-Sarmiento, N. 2019. Plant-growth promotion and biocontrol properties of three Streptomyces spp. isolates to control bacterial rice pathogens. Front. Microbiol. 10:290.   DOI
20 Van Loon, L. C. and Bakker, P. A. H. M. 2006. Induced systemic resistance as a mechanism of disease suppression by rhizobacteria. In: PGPR: biocontrol and biofertilization, ed. by Z. A. Siddiqui, pp. 39-66. Springer, Dordrecht, The Netherlands.
21 Vurukonda, S., Giovanardi, D. and Stefani, E. 2018. plant growth promoting and biocontrol activity of Streptomyces spp. as Endophytes. Int. J. Mol. Sci. 19:952.   DOI
22 Xu, Y., Zhu, X.-F., Zhou, M.-G., Kuang, J., Zhang, Y., Shang, Y. and Wang, J.-X. 2010. Status of streptomycin resistance development in Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola in China and their resistance characters. J. Phytopathol. 158:601-608.   DOI
23 Ahkami, A. H., White, R. A. III, Handakumbura, P. P. and Jansson, C. 2017. Rhizosphere engineering: enhancing sustainable plant ecosystem productivity. Rhizosphere 3:233-243.   DOI
24 Yang, C.-M. 2010. Assessment of the severity of bacterial leaf blight in rice using canopy hyperspectral reflectance. Precis. Agric. 11:61-81.   DOI
25 Yi, H. S., Yang, J. W. and Ryu, C. M. 2013. ISR meets SAR outside: additive action of the endophyte Bacillus pumilus INR7 and the chemical inducer, benzothiadiazole, on induced resistance against bacterial spot in field-grown pepper. Front. Plant Sci. 4:122.   DOI
26 Zuki, Z. M., Rafii, M. Y., Ramli, A., Oladosu, Y., Abdul Latif, M., Sijam, K., Ismail, M. R. and Mohd Sarif, H. 2020. Segregation analysis for bacterial leaf blight disease resistance genes in rice 'MR219' using SSR marker. Chil. J. Agric. Res. 80:227-233.   DOI
27 Abdul-Baki, A. A. and Anderson, J. D. 1973. Vigour determination in soybean seed by multiple criteria. Crop Sci. 13:630-633.   DOI
28 Adhikari, T. B. and Mew, T. M. 1994. Resistance of rice to Xanthomonas oryzae pv. oryzae in Nepal. Plant Dis. 78:64-67.   DOI
29 Chandrasekaran, M. and Chun, S. C. 2016. Expression of PR-protein genes and induction of defense-related enzymes by Bacillus subtilis CBR05 in tomato (Solanum lycopersicum) plants challenged with Erwinia carotovora subsp. carotovora. Biosci. Biotechnol. Biochem. 80:2277-2283.   DOI
30 Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.   DOI
31 Che Omar, S., Shaharudin, A. and Tumin, S. A. 2019. The status of the paddy and rice industry in Malaysia. Khazanah Research Institute, Kuala Lumpur, Malaysia. 203 pp.
32 Chithrashree, Udayashankar, A. C., Chandra Nayaka, S., Reddy, M. S. and Srinivas, C. 2011. Plant growth-promoting rhizobacteria mediate induced systemic resistance in rice against bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. Biol. Control 59:114-122.   DOI
33 Ferraz, H. G. M., Resende, R. S., Moreira, P. C., Silveira, P. R., Milagres, E. A., Oliveira, J. R. and Rodrigues, F. A. 2015. Antagonistic rhizobacteria and jasmonic acid induce resistance against tomato bacterial spot. Bragantia 74:417-427.   DOI
34 Furumo, N. C. and Furutani, S. 2008. A simple method for assaying total protein, polyphenol oxidase and peroxidase activity from 'Kaimana' Litchi chinensis Sonn. J. Hawaii. Pac. Agric. 15:1-7.
35 Hata, E. M., Sijam, K., Yusof, M. T. and Zulperi, D. 2019. Occurrence of Xanthomonas oryzae pv. oryzicola causing bacterial leaf streak disease of rice in different state of Malaysia. J. Plant Pathol. 101:785-786.   DOI
36 Gopalakrishnan, S., Vadlamudi, S., Bandikinda, P., Sathya, A., Vijayabharathi, R., Rupela, O., Kudapa, H., Katta, K. and Varshney, R. K. 2013. Evaluation of Streptomyces strains isolated from herbal vermicompost for their plant growth-promotion traits in rice? Microbiol. Res. 169:40-48.   DOI
37 Hastuti, R. D., Lestari, Y., Suwanto, A. and Saraswati, R. 2012. Endophytic Streptomyces spp. as biocontrol agents of rice bacterial leaf blight pathogen (Xanthomonas oryzae pv. oryzae). HAYATI J. Biosci. 19:155-162.   DOI
38 Hata, E. M., Sijam, K., Mior Ahmad, Z. A., Yusof, M. T. and Azman, N. A. 2015. In vitro antimicrobial assay of actinomycetes in rice against Xanthomonas oryzae pv. oryzicola and as potential plant growth promoter. Braz. Arch. Biol. Technol. 58:821-832.   DOI
39 Herman, T., Murchie, E. H. and Warsi, A. A. 2015. Rice production and climate change: a case study of Malaysian rice. Pertanika J. Trop. Agric. Sci. 38:321-328.
40 International Rice Research Institute. 1996. Standard evaluation system for rice. 4th ed. International Rice Research Institute, Manila, Philippines. 52 pp.
41 Jha, C. K. and Saraf, M. 2012. Evaluation of multispecies plantgrowth-promoting consortia for the growth promotion of Jatropha curcas L. J. Plant Growth Regul. 31:588-598.   DOI
42 Ji, G.-H., Wei, L.-F., He, Y.-Q., Wu, Y.-P. and Bai, X.-H. 2008. Biological control of rice bacterial blight by Lysobacter antibioticus strain 13-1. Biol. Control 45:288-296.   DOI