• 복식문화연구
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• 제12권5호
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• pp.737-742
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• 2004

The purpose of this study is restoration for tearing strength of the durable press (DP) finished 100% cotton fabric by enzymatic treatment. Dimethylol Dihydroxy Ethylene Urea (DMDHEU) was used as a DP finish chemical. Enzymes (cellulase, pectinase, protease, lipolase) were selected based on their specific reaction activities. Ideal application of the enzymes for this work was to remove cross-links created by DMDHEU on the surface of the fibers to offer migration property between microstructures of cellulose, yet cross-links that exist inside of the fibers are still remained to impart effect of wrinkle resistance. Physical characteristics (tearing strength, wrinkle recovery, FT-IR) of enzyme treated samples were measured and compared. It was found out that, in case of enzyme treatment, most of enzymes didn't have a great effect on tearing strength, but, in case of Protease, tearing strength increased at DMDHEU 2% treatment. As a result of an experiment on wrinkle recovery of the textiles treated with enzyme making density of DMDHEU different whenever respective experiment was made, it was discovered that density of DMDHEU increased as wrinkle recovery increased and, in the relation to enzyme treatment especially in Lipase enzyme treatment, the lesser density of DMDHEU, the more wrinkle recovery increased.

• 한국약용작물학회지
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• 제21권4호
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• pp.262-267
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• 2013

The purpose of this study was to research for anti-oxidation and anti-wrinkle effects of Acar mono Sap (AM). To cosmetic effect of AM, safety effect (MTT assay), anti-wrinkle effect (elastase, MMP-1 inhibition assay) and anti-oxidant effect (DPPH assay) were measured. When water extract of AM was used for cell viability, it was over 100% at 6% (6 ml/100 ml in phosphate buffer) concentration. AM showed 45.7% elastase inhibition and 23.7% MMP-1 inhibition at 50% (50 ml/100 ml in phosphate buffer) concentration so that it had good anti-wrinkle characteristic. And AM showed 68.9% antioxidation capacity at 50% concentration by using a DPPH assay. Consequently, AM can be used as natural materials or additives for human skin owing to their beneficial biologic functions, including the anti-wrinkle effect, for cosmetic compositions.

• Toxicological Research
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• 제30권4호
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• pp.283-289
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• 2014

Tea flavonoids and polyphenols are well known for their extraordinary antioxidant activity which is considered important for anti-aging processes in animals. This study evaluated the anti-wrinkle effects of three different kinds of tea (Camellia sinensis) water extracts (CSWEs) including green, white, and black teas using a photoaged hairless mouse model. Data showed that the CSWE-treatment greatly improved skin conditions of mice suffering from UVB-induced photoaging, based on the parameters including the skin erythema index, moisture capacity, and transepidermal water loss. In addition, the wrinkle measurement and image analysis of skin replicas indicated that CSWEs remarkably inhibited wrinkle formation. In histological examination, the CSWE-treated mice exhibited diminished epidermal thickness and increased collagen and elastic fiber content, key signatures for skin restoration. Furthermore, the reduced expression of MMP-3, a collagen-degradative enzyme, was observed in the skin of CSWE-treated animals. Interestingly, comparative data between green, white, and black tea indicated that the anti-wrinkle activity of white tea and black tea is equally greater than that of green tea. Taken together, these data clearly demonstrated that CSWEs could be used as an effective anti-wrinkle agent in photoaged animal skin, implying their extended uses in therapeutics.

• 대한화장품학회지
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• 제28권1호
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• pp.71-79
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• 2002

In the aged skin especially in the face and eyelid, deep and slight wrinkles are one of the remarkable phenomena of aging and the cause of wrinkle is various. Among the cause of wrinkles an oxidative stress plays an important roles in wrinkle formation process. It caused the lipid peroxidation of cell membrane, the increase of the MMPs(MatrixMetalloProteinase) gene expression and cellular DNA damage. These ROS induced materials may cause the degradation of collagen matrix system in the dermis and cause the formation of skin wrinkle. So, it is very important for protecting skin wrinkle formation to regulate ROS activity. In this study, we developed one active ingredient having multi functional activities such as activation of collagen synthesis, inhibition of MMPs activity, lipid peroxidation and free radical scavenging activity and inhibition of free radical induced DNA damage in vitro. Pericarpium castaneae extracts showed collagen synthesis increase in Normal Human Fibroblast and the inhibition of elastase activity (IC$\_$50/ of Elastase: 43.9$\mu\textrm{g}$/㎖). It showed also anti-oxidative activity (IC$\_$50/ : 48$\mu\textrm{g}$/㎖) and free radical scavenging activity(IC$\_$50/: 7.6$\mu\textrm{g}$/㎖). Conclusively, Pericarpium castaneae extracts may be used as an ingredient for new anti-wrinkle cosmetics.

• 대한화장품학회지
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• 제35권4호
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• pp.317-323
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• 2009

지속적인 자외선 노출은 사람의 피부에 주름 형성을 유발한다. 본 연구에서 생강나무 추출물이 광노화에 의한 피부 주름 형성의 개선에 미치는 효능을 검증해 보고자 하였다. 우선 사람 섬유아세포를 이용하여 생강나무 추출물의 세포증식과 타입 I 콜라겐의 생합성 활성을 측정하였다. 그 결과 생강나무 추출물에 의한 세포증식과 타입 I 콜라겐의 생합성능은 대조군과 비교하여 각각 33.8 %와 91.8 % 증식함을 보였다. 동물실험에서는 SKH-1 무모쥐에 일주일에 3번 UV를 조사하면서 5 % 생강나무 추출액을 국부적으로 도포하였다. 10주 후에는 각각의 무모쥐의 피부 모사판을 제작하여 관찰하였다. 광노화에 의한 주름형성에 미치는 영향을 알아보고자 UVB를 무모쥐의 피부조직에 조사한 후 생강나무 추출액을 도포하여 피부 상태를 관찰하였다. 모사판을 접사카메라를 이용하여 관찰한 결과 5 % 생강나무 추출액의 도포는 생강나무가 포함되지 않은 도포액을 도포한 대조군에 비해 UV에 의해 생성되는 주름 형성 억제에 영향을 주는 것으로 나타났다. 이러한 결과로 생강나무 추출액의 도포는 광노화에 의한 피부주름 생성을 억제하고 피부를 보호하는 효과가 있을 것으로 판단된다.

• 대한화장품학회지
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• 제29권2호
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• pp.37-65
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• 2003

새로운 주름개선 효능물질을 개발하기 위하여 여러 가지 세포배양 모델을 이용한 in vitro 효능물질의 검색방법을 이용하여 후보물질을 도출하고 있다. 이처럼 In vitro에서 선별된 주름개선 후보물질은 사람피부에 직접 도포 하였을 때 in vitro에서 와 는 달리 주름개선 효능이 없는 경우가 많이 있다. 즉 in vitro 검색결과와 사람피부에서의 효능은 현실적으로 큰 차이를 보인다는 것이다. 그러나 모든 효능 후보물질을 사람 피부에 직접 검사할 수는 없는 현실적 어려움이 있다. 이러한 애로점을 해결하기 위하여, 동물모델이나, 인공피부배양모델 등을 이용하는 추세이다. 이번 강의에서, 본인은 동물모델과 in vitro에서 효능 측정방법을 상세하게 리뷰 하고 각 방법에 대해 토의할 것이며, 임상 전 시험 결과를 보고 할 것이다. 그 중 특히 동물 모델은 주름개선 효능물질의 효능을 평가하는데 좋은 대상이 될 것이다.

• 융합정보논문지
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• 제11권6호
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• pp.181-187
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• 2021

화장품 시장은 2026년의 초고령사회(Super aged society)가 예측되는 만큼 타 산업과 비교하여 지속적 시장 창출이 기대되는 산업이고, 우리나라 역시 고령화 시대의 진입 속에서 항노화에 관한 관심이 증대되고, 이에 관한 결과로 기능성 화장품에 대한 수요가 증가가 나타났다. 특히, 노년층을 위한 기능성 성분 소재에 관한 연구가 증가하고 있다. 소재 개발의 연구는 화장품 산업에 있어서 신제품 개발로 이어지는 아주 중요한 과정이다. 본 연구는 학술정보시스템(Research information Shining Service, RISS)을 이용하여 국내 천연주름 개선 기능성 화장품 소재개발을 주제로 한 연구를 중심으로 주름 개선화장품의 연구 방향에 대한 여러 가지 주름생성 저해 방법을 실험한 결과와 신소재에 대한 안전하면서도 주름 개선기능이 탁월한 천연 소재 주름 개선 기능성 성분에 관한 최신 연구 동향을 살펴보았다.

• 한국약용작물학회지
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• 제22권3호
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• pp.217-222
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• 2014

This study was to investigate anti-skin wrinkle effect of aronia extracts by extraction processes. The 70% ethanol extract by ultrasonification process (UEE) showed highest DPPH scavenging activity of 90.4% in order water extract (WE), 70% ethanol extract (EE) was measured 84.2%, 84.3%. collagen production measured $245ng/m{\ell}$ from UEE. WE was measured $53.5ng/m{\ell}$ and EE was measured $224.4ng/m{\ell}$. MMP-1 production was observed lowest $22.5pg/m{\ell}$ at UEE. MMP-1 production of EE was $34.6pg/m{\ell}$ and WE was $102.3pg/m{\ell}$. These results were found the highest antioxidant and anti-wrinkle effect at UEE among three processes. It was also confirmed that anti-skin wrinkle activities of the aronia extract was strongly correlated with anti-oxidant activities due to high amounts of antocyanins in the extract.

• 한국정밀공학회지
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• 제32권6호
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• pp.577-585
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• 2015

We fabricated multi-scale such as macro-, micro-, and multi-scale wrinkles by using repetitive volume dividing (RVD) method and thermal curing process. Also wrinkle surface was modified with coating of a self-assembled monolayer (SAM). We measured the contact angle of each wrinkled surface, and observed the behavior of droplets on sloping surface. Through experimental study, we found out that the contact angle was much higher in case of multi-scale and SAM coated wrinkles. And micro-scale wrinkle showed a high contact angle comparing with that of macro-scale wrinkle. Dynamic behaviors of a water droplet like sliding velocity on diverse wrinkled surfaces were dependent on their static contact angles. These results showed that hydro-dynamic characteristics were changed depending on the wrinkle structure and the material forming the wrinkle. These dynamic characteristics can be utilized in bio-chip, microfluidics, and many others in order to control easily chemical reactivity.

• 약학회지
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• 제45권3호
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• pp.310-319
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• 2001

To compare skin irritation and cytotoxicity of anti-wrinkle agents, we examined skin irritation of six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB) in New Zealand white rabbit. Cytotoxicity of these agents was determined by MTT [tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] at multi-time points in cultured HaCaT cell, a human immortalized keratinocyte cell. We then analyzed correlation between skin irritation and cytotoxicity by spearman's rank correlation analysis. All trans-retinoic acid showed the highest primary irritation index (0.92) in skin irritation test. Being all the six agents not irritant, retinal showed the most cytotoxic agents. The correlation between skin irritation and cytotoxicity ($IC_{50}$/ at different time point was 0.814, 0.757, 0.814 and 0.7 at 3, 24, 48 and 72 h, respectively. We also fecund that IC$_{20}$ and IC$_{80}$ of these agents showed similar correlation with skin irritation. These results therefore demonstrated that there is close correlation between skin irritation and cytotoxicity $IC_{50}$/ value by MTT in HaCaT cell at early time points by anti-wrinkle agents or IC$_{20}$ value. $IC_{50}$/ at earily time point or IC$_{20}$ values may be reliable alternative determinant of skin irritation.n.