• Applied Microscopy
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• v.28 no.3
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• pp.329-343
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• 1998

Early wound healing responses in the skin of the Korean fire bellied toad, Bombina orientalis, were examined using histochemical , scanning and transmission electron microscopical techniques. Cutaneous wounds were induced by sharp razor blade , and maintained in special cages for up to 3 days. By this treatment whole parts of epithelium and dermis were profoundly affected. After injury, retraction of damaged surface of the skin was observed. At 3 hours after injury, poly-band was form by mucous substance. The wound skin consisted of mucous gland secreted mucous substance which flows into wound cavity and the wound area was sealed by mucous substance which form thick layer at 6 hours. It appeared that natural disposition showed acid and neutral mucous substance by reaction of PAS and Alcian blue (pH 2.5) staining. After formation of the poly-band, epithelial cells were formed by migration of regenerating epithelial cells. These cells moved over the wound surface by migration at 12 hours after wounding. Poly-band was much increased in thickness and migrating cells were completely formed wound closure at 24 hours post-wounding. After 72 hours regenerated epithelial layer consists of four or five cell layers which are similar to clear, granular, and spinous layer.

• Natural Product Sciences
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• v.6 no.2
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• pp.73-78
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• 2000

The wound healing potential of the methanol extract of Hypericum mysorense Wight and Arn. Leaves (Family: Hypericaceae) was evaluated on different experimental models of wounds in rats. The methanol extract of leaves of Hypericum mysorense (HMM), in the form of ointment in two different concentrations (5% and 10% w/w ointment of aerial part extract in simple ointment base) was evaluated for wound healing potential in excision wound model and incision wound model in rats. Both the concentrations of the methanol extract ointment showed significant responses in both the wound types tested when compared with the control group. The effect produced by the extract ointment, in terms of wound contracting ability, wound closure time, regeneration of tissues at wound site, tensile strength of the wound and histopathological characteristics were comparable to those of a standard drug Nitrofurazone ointment.

• The Korean Journal of Pain
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• v.34 no.2
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• pp.165-175
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• 2021

Background: Management of pain from open wounds is a growing unmet healthcare need. However, the models available to study pain from wounds or to develop analgesics for the patients suffering from them have primarily relied on incisional models. Here, we present the first characterized and validated model of open wound pain. Methods: Unilateral full-skin excisional punch biopsy wounds on rat hind paws were evaluated for evoked pain using withdrawal responses to mechanical and thermal stimulation, and spontaneous pain was measured using hind paw weight distribution and guarding behavior. Evaluations were done before wounding (baseline) and 2-96 hours post-wounding. The model was validated by testing the effects of buprenorphine and carprofen. Results: Pain responses to all tests increased within 2 hours post-wounding and were sustained for at least 4 days. Buprenorphine caused a reversal of all four pain responses at 1 and 4 hours post-treatment compared to 0.9% saline (P < 0.001). Carprofen decreased the pain response to thermal stimulation at 1 (P ≤ 0.049) and 4 hours (P < 0.011) post-treatment compared to 0.9% saline, but not to mechanical stimulation. Conclusions: This is the first well-characterized and validated model of pain from open wounds and will allow study of the pathophysiology of pain in open wounds and the development of wound-specific analgesics.

• Molecules and Cells
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• v.37 no.9
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• pp.637-643
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• 2014

Wound healing is a complex multi-step process that requires spatial and temporal orchestration of cellular and non-cellular components. Hypoxia is one of the prominent microenvironmental factors in tissue injury and wound healing. Hypoxic responses, mainly mediated by a master transcription factor of oxygen homeostasis, hypoxia-inducible factor-1 (HIF-1), have been shown to be critically involved in virtually all processes of wound healing and remodeling. Yet, mechanisms underlying hypoxic regulation of wound healing are still poorly understood. Better understanding of how the wound healing process is regulated by the hypoxic microenvironment and HIF-1 signaling pathway will provide insight into the development of a novel therapeutic strategy for impaired wound healing conditions such as diabetic wound and fibrosis. In this review, we will discuss recent studies illuminating the roles of HIF-1 in physiologic and pathologic wound repair and further, the therapeutic potentials of HIF-1 stabilization or inhibition.

• Perspectives in Nursing Science
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• v.2 no.1
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• pp.48-60
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• 2005

The relationship of psychological stress to human health is of interest to health care providers and researchers in the field of psychoneuroimmunology. The effect of stress on wound healing is a sub-component of study within the larger context, with relevance to both wounds that are acute and chronic in nature. Data from several studies that explore the influence of stress on events early in the trajectory of wound healing suggest that activation of both the sympathetic nervous system and the hypothalamic-pituitary-adrenal axis are involved. There is consistent evidence that psychological stress is associated with dysregulation of immune and other responses to tissue injury that are required for healing and also to the final wound healing result. Current data pertinent to psychological stress and its wound healing consequences is reviewed and a biopsychosocial framework for future studies in this area is suggested and described.

• Journal of the Korean Society of Visualization
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• v.18 no.2
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• pp.10-17
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• 2020

The curvature of wound boundaries has been identified as a key modulator that determines a type of force responsible for cell migration. While several studies report how certain curvatures of the boundary correlate with the rate at which the wound closes, it remains unclear how these curvatures are spatiotemporally formed to regulate the healing process. We investigated the dynamic changes in the boundary curvatures by visualizing cell migration patterns. Locally, cells at the convex boundary continuously move forward with transmitting kinetic responses behind to the cells away from the boundary, and cells at the concave boundary exhibit dramatic contracting motion, like a purse-string, when they accumulate enough negative curvatures to gain the thrust toward the void. Globally, the dynamics of boundary geometries are controlled by the diffusive flow of cells driven by the density gradient between the wound area and the cell layer.

• Archives of Reconstructive Microsurgery
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• v.11 no.2
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• pp.173-178
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• 2002

Wound healing is the result of interaction of normal cellular and biochemical responses that restore the interrupted anatomical structure in limited period. When any response of them is impaired, it results in chronic wound. The factors that influence the wound healing process is not only limited to the fundamental disease of the individual but also the local factors, especially various growth factors secreted from the various cells involved in tissue regeneration have important role. Recent reports that the chronic wounds are depleted of these growth factors have led active studies on the alteration of local wound environment with manipulation of the growth factors and the its application in management of chronic wounds. We investigated the effect on the chronic wounds in 10 patients with various pathologic conditions to suggest the appropriate application and guideline of the indication. The chronic wounds resulting from various causes in 10 patients were treated with rhPDGF gel and good wound care. All the chronic wounds were located on the lower extremity and the average diameter was 2.5 cm. 7 patients were completely cured within 8 weeks, however the patient who received previous radiation therapy the healing was delayed to 14 weeks. Two patients with vascular ulcer were not cured with rhPDGF alone. Local application of rhPDGF has yielded complete cure of the chronic wound in 70% of the patients within 2 months. The author suggests that it would be an effective alternative treatment modality of chronic wound when it is applied with good wound care and appropriate indication.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.218.1-218
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• 1998

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.134.1-134
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• 1996

No Abstract, See Full Text

• Korean Journal of Environmental Agriculture
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• v.15 no.1
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• pp.77-85
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• 1996

Chestnut(Castanea crenata) were trunk-injected by two methods to check the changes around the injection wound. In September 1993, high concentration of oxytetracycline(OTC) was injected through the injection wound of 1cm diameter and low concentration of OTC through 0.5cm diameter. Trunk diameter of injected trees ranged from 10cm to 20cm. All trees were in their vigorous conditions. Tree reaction was examined in June, 1994. None of them showed any sign of decay by the time. However, under the bark, sapwood was remarkably discolored. But, more severe discoloration was found in 1cm-injection wound than in 0.5cm one. Sapwood of some trees split from the injection wound, and the split was longer in 1cm-injection wounded trees than in 0.5cm trees. From the split, callus grew out and the split was closing. In this kind of trunk injection, the damage was more severe in 1cm-injection wound than in 0.5cm-injection wound.