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http://dx.doi.org/10.5407/jksv.2020.18.2.010

Identification of boundary migration during the wound healing through the visualization of cell migrations  

Jeong, Hyuntae (Department of Mechanical Engineering, KAIST)
Lee, Jaesung (Department of Mechanical Engineering, KAIST)
Shin, Jennifer Hyunjong (Department of Mechanical Engineering, KAIST)
Publication Information
Journal of the Korean Society of Visualization / v.18, no.2, 2020 , pp. 10-17 More about this Journal
Abstract
The curvature of wound boundaries has been identified as a key modulator that determines a type of force responsible for cell migration. While several studies report how certain curvatures of the boundary correlate with the rate at which the wound closes, it remains unclear how these curvatures are spatiotemporally formed to regulate the healing process. We investigated the dynamic changes in the boundary curvatures by visualizing cell migration patterns. Locally, cells at the convex boundary continuously move forward with transmitting kinetic responses behind to the cells away from the boundary, and cells at the concave boundary exhibit dramatic contracting motion, like a purse-string, when they accumulate enough negative curvatures to gain the thrust toward the void. Globally, the dynamics of boundary geometries are controlled by the diffusive flow of cells driven by the density gradient between the wound area and the cell layer.
Keywords
Wound healing; Collective cell migration; Digital Particle Image Velocimetry; Wound boundary geometry;
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