• 미생물학회지
• /
• 제52권4호
• /
• pp.398-405
• /
• 2016

이전의 연구에서, bacterioferritin comigratory protein (BCP)을 인코딩하는 Schizosaccharomyces pombe의 구조유전자를 shuttle vector인 pRS316에 클로닝하여 BCP 과잉발현 플라즈미드인 pBCP10을 제조한 바 있다. 본 연구에서는, 플라즈미드 pBCP10을 사용하여 고온 스트레스에 대한 BCP의 열저항적 성질을 평가하였다. 대수기의 초기까지 성장시킨 S. pombe 세포의 배양 온도를 $30^{\circ}C$에서 $37^{\circ}C$나 $42^{\circ}C$로 전이시키는 경우, pBCP10 함유 S. pombe 세포가 벡터 대조 세포보다 $37^{\circ}C$와 $42^{\circ}C$ 모두에서 유의하기 더 잘 성장하였다. 높은 배양 온도로 전이한 뒤 6시간에서, pBCP10 함유 S. pombe 세포가 벡터 대조 세포보다 낮은 활성산소종(ROS)과 일산화질소(NO)의 지표로 측정된 아질산염(nitrite) 함량을 갖고 있음이 확인되었다. 온도 전이 뒤에, 총 글루타치온(total glutathione) 함량과 총 수퍼옥사이드 디스뮤타제(superoxide dismutase) 활성은 대응되는 벡터 대조 세포보다 pBCP10 함유 S. pombe 세포에서 현저하게 높다는 사실도 확인되었다. 종합하면, S. pombe BCP는 열저항적 역할을 보유하는 데, 활성산소종과 일산화질소에 대한 하강시키는 활성과 총 글루타치온과 수퍼옥사이드 디스뮤타제 등의 항산화 성분들을 상승시키는 활성, 즉 종합적으로 열안정성을 유지하는 활성에 근거하는 것으로 추정되었다

• 콘크리트학회논문집
• /
• 제21권6호
• /
• pp.763-771
• /
• 2009

최근 장경간에 대한 요구가 증가됨에 따라 수평부재는 극한강도 뿐만 아니라 과도한 처짐이 중요한 문제로 인식되고 있다. 따라서 건축물의 경우에 처짐을 해결할 수 있는 좋은 대안으로서 비부착긴장재를 사용한 포스트텐션 구조의 적용이 늘어가고 있다. 그러나 대부분의 기존 연구들은 비부착긴장재를 사용한 프리스트레스트 휨부재의 극한강도에 국한되고 있기 때문에 처짐과 같은 사용성 검토에는 적용이 불가능 하거나 매우 어려운 실정이다. 따라서 이 연구는 비부착긴장재를 사용한 포스트텐션 부재의 균열 전 초기 거동, 균열 후 및 사용하중 상태에서의 거동 및 극한강도까지 적용이 가능한 긴장재의 응력거동 예측모델을 제안하고, 기존 실험결과와의 비교를 통하여 제안 모델의 적용성 및 정확도를 검증하고자 하였다. 다양한 부재 특성을 가진 실험 결과와의 비교를 통하여 제안된 응력거동 예측모델은 보강지수 및 하중형상에 관계없이 하중단계별 긴장재의 응력 및 강도를 매우 정확하게 예측함을 확인할 수 있었다. 특히, 제안된 모델은 다양한 재하형태의 영향을 잘 반영하였으며, 휨 부재를 보강할 때 쉽게 발생할 수 있는 과보강 부재에 대해서도 무리없이 적용 할 수 있을 것으로 판단된다.

• 한국건설관리학회논문집
• /
• 제10권1호
• /
• pp.114-125
• /
• 2009

본 연구에서는 확률적 LCC 및 확률적 성능평가를 고려한 설계 VA분석 방법을 제안하였으며, 신뢰성 해석의 개념을 도입하여 신뢰도를 확보한 의사결정을 지원하고자 하였다. 상기와 같은 목적과 방법에 따라 진행된 본 연구의 결과를 요약하면 다음과 같다: 1) 기능정의가 완료된 이후 도출된 대안들의 대안평가시 신뢰성 있는 분석을 위하여 개선된 설계VA 절차 와 가치상태함수를 정식화하였으며, 가치평가절차에서의 일관성 및 효율성을 확보하기 위하여 평가지표방안을 제시하였다. 2) 교량의 LCC분석을 위한 DB를 수집 및 분석하였다. 자료의 수집범위는 기존에 수행된 교량의 LCC분석 연구 문헌을 기초로 하여 분석하였으며, 분석결과에 대한 신뢰도 확보 및 수집된 자료의 불확실성 처리를 위해 MCS 기법을 적용하였다. 3) 대안별 성능평가를 위한 가중치 및 평가등급과 LCC 분석을 위한 LCC 분석모델, 분석기간, 할인율, 사용자비용, 안전점검 및 안전진단비용에 대한 조건을 제시하였다. 끝으로, 사례대상인 "OO대교 및 연결도로 건설공사 실시설계" 프로젝트에 대한 VA수행 사례를 중심으로 타당성을 검토하고 결론을 도출하였다.

• 동의신경정신과학회지
• /
• 제23권1호
• /
• pp.93-114
• /
• 2012

Objective : This study aimed to review the performance of traditional herbal prescriptions for treating dementia and present a strategy for research on dementia therapy utilizing herbal medicine. Methods : A definition was made to clarify the technology regarding the development of herbal prescriptions for treating dementia. The queries were compounded based on the initial keywords provided by experts in the field, then applied to the Web of Science database search engines from January 1986 to September 2011 to search related scientific articles. Before performing the analysis, papers were extracted from the initial search reviewed by experts and 80 articles were selected. Then, the selected papers were analyzed in terms of publish year, country, and type of herbal prescriptions. Furthermore, the research performance evaluation for treating dementia by herbal prescriptions was also created in terms of country and organization based on forward citation analysis. In addition to, for the evaluation regarding research quality, we classified and reviewed papers into two types: clinical studies and experimental studies. Results : According to the quantitative information analysis of 80 articles, the number of papers has increased by 21.9% per the yearly mean from 1995, and Japan had the largest portion within this research field. There were 34 kinds of traditional herbal prescriptions, among them Ukgansan had the highest number of studies followed by Jodeungsan, Dangkisoosan and so on. In addition, quality index as calculated by cites per paper is higher than average in Switzerland, Turkey and Japan. In the view of the evaluation on quality there were 12 clinical studies, 8 RCT reported that herbal prescriptions had efficacy at cognition, behavioral & psychological symptoms (BPSD) and activity of daily life (ADL) in various type of dementia. In experimental studies most of the studies were performed using animal models. The studies using Ukgansan were aimed at improving BPSD. The papers studied with Jodeungsan and Dangkisoosan targeted vascular dementia. Conclusions : In this study, research to develop traditional herbal prescriptions for treating dementia has the potential to improve symptoms since herbal medicines work as both multi-function and multi-target in dementia with multiple pathological or neurotoxic pathways. Therefore, the results of the research should be used in order to establish strategies to develop technology for treating dementia with traditional herbal prescriptions in the future.

• Journal of Preventive Medicine and Public Health
• /
• 제42권5호
• /
• pp.315-322
• /
• 2009

Objectives : Knowledge about the management status of diabetic melitus (DM) is essential to improve diabetic management. Moreover, low income is associated with poor adherence to treatment and increased mortality. This study was performed to evaluate the management status of DM in low-income patients in a rural area. Methods : We enrolled 370 patients with type 2 DM living in Gokseong county, JeollaNamdo. A well-trained examiner measured the height, weight, waist circumference, blood pressure, total cholesterol, triglyceride, high density lipoprotein cholesterol, fasting blood sugar and glycosylated hemoglobin (HbA1c) levels. Carotid ultrasonography was used to measure carotid artery carotid artery intima media thickness (IMT) and plaque. anklebrachial index (ABI) was used to evaluate peripheral artery disease. A fundoscopic examination was performed to evaluate diabetic retinopathy. A history of diabetes complications and health-related questionnaires were also completed. Results : The age of diabetic subjects was 68.7$\pm$8.7 years and the duration of diabetes was 8.9$\pm$8.2 years. Most (63.5%) had hypertension, and 45.7% had triglycerides below 150 mg/dl, 38.1% had low density lipoprotein cholesterol (LDL) cholesterol below 100 mg/dl, 48.7% had urine albumin to creatinine ratio (UACR) below 30 mg/g. Less than half (45.9%) achieved the goal of HbA1c less than 7% suggested by the American Diabetes Association (ADA). 10.6% had peripheral vascular disease, 11.9% had retinopathy, and 60.8% had chronic kidney disease. Conclusions : DM management in low income patients is very poor and requires further work to improve.

• 전자공학회논문지SC
• /
• 제47권2호
• /
• pp.43-49
• /
• 2010

자율신경계는 불수의적 활동 기능을 조절해 주는 신경계통으로, 자율신경계의 활성 정도를 정량적으로 평가하기 위해 심박 변이도가 이용된다. 그러나 심박 변이도는 환경 변화나 심리 상태의 변화 등에 민감하게 반응하며, 자세와 수면 박탈에 의해서도 심박 변이도가 달라질 수 있다. 특히 전자파 자원자 연구에서는 자각 증상 및 인지 여부에 의한 수면 박탈로 심박 변이도가 달라질 수 있다. 이에 본 연구에서는 자세에 따른 수면 박탈 횟수와 심박 변이도를 30분간 6 stage에서 측정하였고, 앉은 자세와 누운 자세에서 측정된 심박 변이도를 비교하여 앉은 자세나 누운 자세에서 심박 변이도를 측정하는 실험에 어떠한 자세가 적합한지 결정하고자 하였다. 심박 변이도 분석은 전력 스펙트럼을 이용한 주파수 영역에서의 LF (low frequency) / HF(high frequency)를 이용하였고, stage 1을 100%로 하여 개인 및 성별에 따른 초기 LF/HF 차이를 상쇄시켜 주었다. LF/HF 증가는 교감 신경의 활성 증가를 나타내고 감소는 교감 신경의 활성 감소를 나타낸다. 교차 분석 결과 수면 박탈은 자세의 영향을 받는 것으로 나타났으며 (p=0.002), LF/HF에 대하여 자세만을 변수로 고려하였다. 앉은 자세에서 수면 박탈 횟수는 모든 stage에서 누운 자세보다 유의하게 적었다 (p<0.05). 자세는 LF/HF에 유의한 영향을 끼쳤으며 (p=0.033), 앉은 자세에서는 stage 1과 비교하여 LF/HF가 stage 4, 5, 6에서 유의하게 증가하였고 (p<0.05), 누운 자세에서는 stage 1과 비교하여 모든 stage에서 LF/HF가 유의하게 증가하였다 (p<0.05). 따라서 앉은 자세가 누운 자세보다 수면 박탈 횟수가 적고 LF/HF 변화가 작기 때문에 피험자가 깨어 있어야 하는 심박 변이도 측정 실험에 있어서 더 적합한 자세라고 사료된다.

• 산업경영시스템학회지
• /
• 제17권32호
• /
• pp.255-266
• /
• 1994

This study does present things to suggest to foreign multinational corporation or domestic corporation through the empirical study on the standardization level. If we summary this study, it is as follows. In chapter I, we did institute the problems and explain the objects and methods and system of the study. In chapter II, we did explain the universality of the culture and international advertisement. Next, we did explain the background of doing specialization strategy and standardization strategy. Also we did study the previous study about the arguements for and against these. Lsat we did institute the hypothesises as follows. - Hypothesis 1 $H_0$ : The use of westerner appeal means has been increased than 11 years before and is able to increase continuously. - Hypothesis 2 $H_0$ : The use of westerner model and knowned has been increased than 11 years before and is able to increase continuously. - Hypothesis 3 $H_0$ : The use of the English language has been increased than 11 years before and is able to increase continuously. - Hypothesis 4 $H_0$ : The use of the west short piece and background has been increased than 11 years before and is able to increase continuously. In chapter III, we did refer to the method of this study. First, we did adjust the measure of standardization index, and we did work coding it, such after the two coder did check the freguency of the appeal meanstype about the advertisement of four daily report. Then we did select eight production as high involvement production and seven production as low involvement. We did test the coder's trust degree on the such sample to be selected through the 't-test' of the SAS statistic program and regression analysis through the spss/$pc^+$ statistic program to approve the this study's hypothesises. In chapter IV, we did analysis the results of the empirical study on the high or low involvement productions. First, the results of t-test to approve the coder's trust degree is that it is same statistically attentionly the distribution of the sample between coder at 10% attention level. Next, the results of the regression analysis on eight hypothesises are as fellows. In case of high involvement production we are able to speak that the use of the English language appeal among the eight appeals is only standardized. And incase of low involvement production, we are able to speak that the use of the hard-sell appeal among the eight appeds is only standardized. Therefore we did conclude that the standardization level of Korea newspaper advertisement is low. Also this suggests that it will do plan and do the specialzation strategy or localization strategy foreign multinational corporation or domestic corporation in Korea newspaper advertisement. In chapter V, we do present the boundary of this study and new study methods.

• 한국수정란이식학회지
• /
• 제28권3호
• /
• pp.297-302
• /
• 2013

Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of genetically elite populations. Castration, catastrophic injury, sudden death or any other event that makes semen collection or mating impossible may prematurely terminate a stallion reproduction. Stallion epididymal spermatozoa vary widely in the loss of progressive motility, acrosomal integrity, and viability during freezing and thawing. The objective of this work was to investigate the effect of (1) freezing package types on cryopreservation efficiency, (2) thawing temperatures (37, 56 or $70^{\circ}C$) on Computer Assisted Sperm Analysis (CASA) parameters and (3) post-thawing incubation time (0, 1, 2 or 4h) on castrated stallion epididymis. Post-thawed sperm motility ranged between 59.69% and 64.28% ($56^{\circ}C$ and $37^{\circ}C$) in various thawing temperatures. When stallion epididymis sperm was frozen, straw was better than freezing tube on VCL (Velocity of Curvilinear Line) and VAP (Velocity of Average Path) parameter. Higher percentage of motility was observed at $37^{\circ}C$ thawing temperature even though no significant difference was observed among various temperatures. The motility, VCL, ALH (Amplitude of Lateral Head displacement), VAP, BCF (Beat-Cross Frequency) and STR (Straightness index) parameter of post-thawed sperm were significantly decreased by increasing the incubation time for all thawing temperatures. The present study showed that type of freezing package (Straw vs. Freezing tube) was not significantly different on cryopreservation efficiency. Furthermore, stallion epididymal spermatozoa frozen-thawed at $37^{\circ}C$ for 1 min resulted the highest proportion of motility and velocity movement. In addition, motility and viability of frozen-thawed stallion epididymal spermatozoa were also decreased by incubation.

• 한국산업보건학회지
• /
• 제15권2호
• /
• pp.104-113
• /
• 2005

A variety of ergonomic assessment methods of lifting tasks known as a major cause of work-related lower back pain have been used. But there is a limited information in choosing the most appropriate assessment method for a particular job and in finding out strengths and weakness of the methods. The purpose of this study was to assess and compare the ergonomic risks of lifting tasks in a marine diesel engine production industry by three lifting ergonomic assessment tools widely used: the National Institute for Occupational Safety and Health(NIOSH) Revised Lifting Equation(NLE), the Washington Administrative Code 296-62-0517(WAC), and the Snook Tables. Lifting index(weight of load/Recommended Weight Limit) of NLE($LI_{NLE}$) was above 1 at 34 tasks(75.6%) of a total number of 45 lifting tasks. LI of WAC($LI_{WAC}$) was above 1 at 11 tasks(24.4 %). LI of Snook Table($LI_{Snook}$) was above 1 at 29 tasks(64.4%). Thus, LI was high in orders of $LI_{NLE}$ > $LI_{Snook}$ > $LI_{WAC}$. There were significantly high correlations among three Lls(p<0.01). The correlation coefficients between $LI_{NLE}$and the other three Lls($LI_{WAC}$ and $LI_{Snook}$) were r=0.93 and r=0.88, respectively. The linear regression equations were y = 0.444x + 0.11(r=0.93) between $LI_{NLE}$ and $LI_{WAC}$, y = 0.93x + 0.008(r=0.88) between LI(NLE) and $LI_{Snook}$. The LI values by WAC was significantly lower than those by the other tools. The compared features, strength and limitation among these tools were described in this paper.

• 한국환경보건학회지
• /
• 제8권1호
• /
• pp.67-80
• /
• 1982

In order to evaluate the psychosomatic health status of metal manufacturing industries workers and their working environments, the present study was conducted from March 1, 1981 to the end of September 1981. The data was obtained from the samples of metal manufacturing industries in Kyung-In Area and their 1, 162 employees. In addition, the 803 urban residents including students, office clerks, and general publics were sampled as control groups to compare with factory employees in psychosomatic analysis. The basic tool employed in the present study was the Todai Health Index (THI) which modified CMI and was developed by Tokyo University Research Team of Japan. The results of the present study were summarized as follows: 1. Working environments of the factories 1) The data shows that fabrication shop produced the highest noise level ranging from 91 to 96 dB (A) and iron and steel shop had the lowest noise level ranging from 81 to 86 dB (A). 2) Dust concentration was the highest in iron foundry shop ($3.8 mg/m^3$) and the lowest in fabrication shop ($1.2 mg/m^3$). 3) WBGT above threshold limit values (T.L.V.) was noted in steel shop (38$\circ$C) and iron foundry shop (34$\circ$C) 4) The concentration of Sulfur dioxide ($SO_2$)was 30.5ppm at steel shop and 12.0ppm at iron foundry shop. 5) The concentration of carbon monoxide (CO) was 140.0ppm at steel shop and 110.5ppm at iron foundry shop. 6) The atmospheric lead concentration was $0.49 mg/m^3$ at soldering shop. 2. The responses of psychosomatic complaints were much higher in steel shops group than in other manu-facturing group, except the response of aggressiveness. 3. The responses of psychosomatic complaints were much higher in industrial workers than in urban residents, except the responses of depression and aggressiveness (p < 0.01 ). 4. The psychosomatic symptoms which industrial workers and urban residents complained frequently were nervousness. agressiveness and lie scale in order. 5. The responses of psychosomatic complaints by sex were much higher in female group than in male group, except the response of aggressiveness. 6. The responses of psychosomatic complaints by age were that both the female and male group showed an increasing tendency in the all items, except the response of depression as age was increasing. 7. The responses of psychosomatic complaints by the length of services were that both the female and male group showed a tendency of increasing in physical symptoms as work years increased. 8. The responses of psychosomatic complaints were higher in unmarried group in the score of nervousness, aggressiveness, mental irritability and irregular life.