• Journal of Forest and Environmental Science
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• v.21 no.1
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• pp.24-33
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• 2005

Fallen needles (8.5kg) of Larix kaempferi were separately collected, extracted with 95% EtOH. EtOH extract was evaporated under reduced pressure, concentrated then successively fractionated with a series of hexane, methylene chloride, ethylacetate and water on a separatory funnel. Then, each fraction was freeze dried. A portion of ethylacetate and water soluble powder were packed on a column chromatography (Sephadex LH-20) eluting with aqueous MeOH and EtOH-hexane mixture. Spectrometric analyses such as NMR and FAB-MS including TLC were performed to characterize the structures of isolated compounds. 5 compounds were isolated from the fallen needles of Larix kaempferi. The antioxidative activities of each fraction and isolated compounds were done by DPPH radical scavenging test.

• Journal of the Korean Wood Science and Technology
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• v.52 no.1
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• pp.13-30
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• 2024

Swietenia mahagoni is one of the commercial timbers in Indonesia. Mahogany heartwood is an important characteristic as it relates to the natural durability and aesthetics of the wood. Lipophilic extractives are known to be involved in the heartwood formation process. Therefore, this study aims to determine the lipophilic compounds associated with heartwood formation. The n-hexane extract from sapwood and heartwood samples (1 to 5 years) was analyzed by gas chromatography-mass spectrometry. The results showed that the content of n-hexane extract ranged from 0.76% to 2.45% based on dry wood. The main group of compounds identified in the lipophilic fraction consisted of sterols (β-sitosterol, stigmasterol, campasterol, and cyclolaudenol), fatty acids (palmitic, oleic, linoleic, and stearic acid), and hydrocarbons (pentadecane, 1-octadecane, hexadecane, cyclotetracosane, cycloeicosane, and cyclooctacosane) after heartwood formation. In addition, the hydrocarbon fraction was the largest, followed by sterols, fatty acids, and 1-heneicosanol. In the radial variation, the distribution of fatty acids was greater in the sapwood than in the heartwood (4-year-old). However, the reverse pattern was found at the age of 5 years. The lipophilic fraction was generally more abundant in the heartwood compared to the sapwood, especially at 5 years of age, with much higher levels than when the heartwood was forming (4 years). These findings show that when the heartwood formation begins, the lipid composition was not fully metabolized at the beginning of heartwood formation compared to 5-year-old trees.

• Journal of the Korean Wood Science and Technology
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• v.34 no.3
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• pp.73-83
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• 2006

The dried needles (1.5 kg) of Abies koreana and Abies holophylla were ground, extracted with acetone-$H_2O$ (7:3, v/v), concentrated, and fractionated with a series of hexane, methylene chloride, ethyl acetate and water on a separatory funnel. Each fraction was freeze dried, then a portion of ethyl acetate soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. The isolated compounds were identified by cellulose TLC, $^1H$, $^{13}C-NMR$, COSY, HETCOR, FAB and EI-MS. The needles of Abies koreana and Abies holophylla contained a large amount of aromadendrin-7-O-${\beta}$-D-glucopyranoside (compound III), polydatin (compound VI), (-)-rhododendrol-2-O-${\beta}$-D-glucopyranoside (compound VII), in addition to a small amount of (+)-catechin (compound I), kaempferol-3-O-${\beta}$-D-glucopyranoside (compound IV), myricetin-3-O-${\beta}$-D-glucopyranoside (compound V), naringenin-7-O-${\beta}$-D-glucopyranoside (compound II). DPPH analysis was also tested to investigate the antioxidative effects on the isolated compounds and (+)-catechin and polydatin were effective.

• Journal of the Korean Wood Science and Technology
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• v.28 no.3
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• pp.70-77
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• 2000

Among four compounds such as oxyresveratrol, resveratrol, morin and afzelechin isolated from the heartwood of Morns bombycis, oxyresveratrol and resveratrol which belong to stilbenoid indicated high antioxidative and antimicrobial activity, respectively. Based on this result, this experiment was carried out to elucidate the relationship between biological activities of stilbenoids and their chemical structures using two isolated and six synthesized ones prepared by Wittig reaction. Antimicrobial activity of 3,5-dihydroxystilbene(pinosylvin) devoid of hydroxyl group of B-ring was the best among the tested stilbenoids. Antimicrobial activities of the stilbenoids showed negative dependency on the number of hydroxyl groups of B-ring, that is, the fewer number of hydroxyl groups of B-ring, the higher antimicrobial activity. On the other hand, antioxidative activities of the stilbenoids indicated a positive relationship with the number of hydroxyl groups of B-ring. In tetrahydroxystilbenoids, antioxidative activities of the compounds possessing ortho-diphenol structure, 2',3,3',5- and 3,3',4',5-tetrahydroxystilbene, were superior to the others. As a result, it was concluded that resveratrol and oxyresveratrol isolated from the heartwood of M. bombycis had antimicrobial and high antioxidative activities, and these activities of stilbenoids were also dependent on the number and position of hydroxyl groups of B-ring.

• Journal of the Korean Wood Science and Technology
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• v.28 no.2
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• pp.32-41
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• 2000

Antimicrobial and antioxidative activities on heartwood extractives from Zelkova serrata were investigated to develop a natural fungicide or preservative. The ethanol extract from Z. serrata was fractionated in the order of petroleum ether, diethylether and ethylacetate to determine antimicrobial activity. The highest antimicrobial activity against the tested microorganisms was found in the petroleum ether soluble fraction. An active antimicrobial compound was isolated from petroleum ether soluble fraction, and identified as 7-hydroxy-3-methoxycadalene by $^1H$-, $^{13}C$-NMR and EI-MS spectrometry. This compound showed higher antifungal activity, but lower antibacterial activity than hinokitiol(${\beta}$-thujaplicin), strong antimicrobial compound isolated from Thujopsis dolabrata. Antioxidative activity was also higher than ${\alpha}$-tocopherol and similar to BHT(butylated hydroxytoluene), one of the strongest synthetic antioxidant. As a result, it was concluded that 7-hydroxy-3-methoxycadalene isolated from Z. serrata had strong antifungal and antioxidative activities.

• Journal of the Korean Wood Science and Technology
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• v.41 no.6
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• pp.566-575
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• 2013

The fruits of Taxus cuspidata were collected, divided into seeds and fruits, and extracted with 95% EtOH. The extracts were evaporated under the reduced vacuum pressure, concentrated, then successively fractionated with a series of n-hexane, dichloromethane, ethyl acetate and water on a separatory funnel to get some freeze dried samples. A portion of the EtOAc (arils:1.65 g, seeds:1.04 g) and $H_2O$ (arils:7 g, seeds:10 g) soluble samples were chromatographed on a Sephadex column using MeOH-$H_2O$ (1:1, 1:3, 1:5, v/v), EtOH-hexane (3:1, v/v) mixture and 100% $H_2O$ as eluting solvents to isolate pure compounds from the fractions. The isolates were developed by cellulose TLC using t-BuOH-HOAc-$H_2O$ (TBA; 3:1:1, v/v/v) and 6% aqueous HOAc. Visualization was done under ultraviolet light and by spraying the vanillin-HCl-EtOH reagent (4.8:12:480, v/v/v). followed by heating. The structures of the isolates were characterized by $^1H$- and $^{13}C$-NMR, DEPT, 2D-NMR, LC/MS and EI-MS spectra. In addition to the NMR and MS spectra, acid hydrolysis and permethylation were used to determine the correct structure of the isolated sugar compound. Their structures were elucidated as (+)-catechin (1), (-)-epicatechin (2), (+)-gallocatechin (3), (-)-epigallocatechin (4) and ${\beta}$-D-fructofuranose-($2{\rightarrow}4$)-O-${\beta}$-D-glucopyranose($1{\rightarrow}4$)-O-${\alpha}$-D-glucopyranose ($1{\rightarrow}2$)-O-${\beta}$-D-fructofuranose (5) on the basis of the above experimental evidences.

• Journal of Korea Foresty Energy
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• v.24 no.2
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• pp.1-9
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• 2005

After manufacturing fermentation system for degrading pig manure using environmentally friendly technique, performance of the system and characteristics of wood chips and pig manure fermented in the system were analyzed. Results from this study shows that proper fermentation temperature($55{\sim}60^{\circ}C$) reached 3days after the system started and degradation rate, which expresses fermentation performance of system, was $180{\iota}$/day. Even as progressing the fermentation of wood chips and pig manure mixture, the amount of extractives drawn out by alkali, and alcohol-benzene and lignin content was not varied. However, ash content in wood was increased. The inorganic compounds in pig manure seem to be transferred into wood chip. On the other hand holocellulose contents in wood were decreased a little. Holocellulose seems to be consumed as the second carbon source in fermentation process. Results through analysis of inorganic- and heavy metal elements contents in wood chips and pig manure fermented in long term process shows that inorganic elements($Ca^{2+},\;Mg^{2+},\;K^+,\;Na^+$ etc.) contents were increased with fermentation time and heavy metal elements(Cd, As, Cu etc.) which cause environmental pollution were not detected. Number of microorganisms including bacteria, actinomycetes, and fungi, the number of C.F.U(Colony Forming Unit) was increased while temperature in fermentation system was abruptly increased.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.41 no.2
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• pp.40-46
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• 2009

The purpose of this study was to estimate natural dyeing properties of Korean traditional paper (Hanji). We dyed the Korean traditional paper with dyestuff which extracted from wood meal of Cotinus coggygria Scop (smoke tree) using hot-water, ethanol and $K_{2}CO_{3}$ solution. As mordants, 0.5% of $AlK(SO_4)_2$, $FeCl_2$, and $CuCH_3(COO)_2$ solution were used respectively. The color of dyed Hanji mainly depended on not the methods of extraction but the kinds of mordant. The dyed Hanji mordant with $AlK(SO_4)_2$ colored vivid yellow, $FeCl_2$ colored dark olive, and $CuCH_3(COO)_2$ colored brown and/or orange. The dyed Hanji with hot-water extractive had the highest K/S value and ethanol and $K_{2}CO_{3}$ solution extractives were followed. The K/S value of dyed Hanji mordant with $AlK(SO_4)_2$ was higher than that of $FeCl_2$ and $CuCH_3(COO)_2$. The dyeing effectiveness of after-mordanting method was superior to the others but sim-mordanting method was the worst.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.41 no.1
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• pp.1-8
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• 2009

Mountain Pine Beetle (MPB) attacked pine was evaluated for pulp quality, chemical and physical properties, and bleachability. Chemical analyses showed that the dehydroabietic acid and total acetone extractives content were higher in the year 3 (grey) attacked MPB chips and lower in the year 5 (grey) attacked MPB chips as compared to a typical SPF (spruce/pine/fir) reference. Lignin and carbohydrate content of the MPB wood chips were comparable to the SPF. Similarly, there was little difference in kappa number, pulp yield and liquor consumption between the 3 and 5 year MPB attacked wood. Likewise there was no significant difference in the resulting tear strength, burst, or tensile strength. There appeared to be an improved bleaching response in the MPB attacked pulp as compared to the SPF reference, but this was accompanied by a slightly lower bleached pulp yield and higher bleach filtrate COD and solids content.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.43 no.5
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• pp.60-67
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• 2011

Hemicelluloses pre-extracted from Korean mixed wood chip were investigated as a wet-end additive. Hemicelluloses dissolved in hot water and alkali solution were isolated by ethyl alcohol precipitation from pre-extractives. They showed molecular weight of 9,000 ~ 27,000 g/mol as revealed by size exclusion chromatography. The reduction of molecular weight through hot water extraction was caused by autohydrolysis. Chemical composition of the hemicelluloses were analyzed with high-performance liquid chromatography and UV-Vis spectroscopy. As the surface charge of isolated hemicelluloses were negative, the adsorption of hemicelluloses onto softwood unbleached kraft pulp fiber was promoted by poly-DADMAC. The physical properties of handsheets increased as the molecular weight of hemicellulose increased. On the other hands, the optical property decreased with hemicellulose adsorption.