• Journal of Forest and Environmental Science
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• v.34 no.5
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• pp.376-381
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• 2018

For the control of oak wilt caused by Raffaelea quercus-mongolicae, an antifungal microorganism, Streptomyces blastmyceticus, was used as a potential agent. Culture suspension of S. blastmyceticus was injected into Quercus mongolicae in the research forest of Kangwon National University by $ChemJet^{(R)}$ trunk injection and Macro-infusion at root flare injection. $Alamo^{(R)}$ (a.i., propiconazole 14.5%), a fungicide currently used for the control of oak wilt in USA, was also treated by both methods to compare the efficacy. For preventive efficacy, culture suspension of the pathogen was inoculated at 1 month after injection of either agent. Tested trees were cut at 3 months after treatment, stained with 1% Fuchsin acid, and then non-conductive area (NCA) and re-isolation frequency (RIF) of oak wilt fungus were compared among treatments. While NCA was the highest as 47.3% in pathogen only treatment, it was the lowest as 16.0% in sterilized water treatment by Macro-infusion. NCAs of Alamo treatment by Macro-infusion and ChemJet injection were 25.3% and 32.1%, respectively. NCA of S.blastmyceticus treatment by ChemJet injection was 32.3%, similar with Alamo treatment's by ChemJet injection. All treatments by either injection method showed significantly lower NCA compared to the pathogen only treatment. These results indicate that S. blastmyceticus injection shows the preventive efficacy against oak wilt disease by suppressing the growth of pathogen injected. NCA of Macro-infusion injection of sterilized water was lower as 16.0%, compared to 21.3% of ChemJet injection. It means that Macro-infusion is more effective in translocation of sterilized water than ChemJet injection by even distribution. RIF from wood discs of treated trees showed high in pathogen only treatment, but relatively low in S. blastmyceticus treatment. RIF results were correlated with NCA results. From the above results, it was confirmed that S. blastmyceticus showed preventive efficacy against oak wilt disease by ChemJet trunk or Macro-infusion at root flare injection.

• Journal of Veterinary Clinics
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• v.22 no.1
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• pp.56-59
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• 2005

An about 5-month-old, female rabbit was presented with pruritus, alopecia and mucopurulent ocular discharge. On physical examination mild to moderate scales on whole body were observed. Dermatological lesions such as alopecia, erythema, papules, pustules and crusts were observed in the eyelids, nose, inner pinna, medial sites of four feet, metacarpal and metatarsal areas, and cranial site of left stifle joint. Also, mild conjunctivitis, blepharitis and keratitis were found. For extensive dermatologic diagnostic evaluation skin scraping, tape stripping, impression smear, combing, wood' light, bacterial culture and fungal culture were performed. Finally, Cheyletiella spp. was found by combing. Many heterophils and eosinophills were appeared in impression smear. The result of fungal culture was negative. Pasteurella spp was cultured. Definitive diagnosis of Cheyletiellosis and secondary Pasteurella spp infection were established. The rabbit was treated with 6 mg/kg of selamectin topically every two weeks and restricted in cage for one month. During one month of initial treatment, clinical signs such as pruritus, alopecia, scales, papules, pustules and crusts were remarkably improved. However, in spite of good therapeutic response of selamectin the rabbit was dead suddenly at 22 days after second administration of selamectin. This case showed that selamectin was possibly effective for cheyletiellosis in rabbit; safety of selamectin for rabbit was not identified.

• Research in Plant Disease
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• v.20 no.3
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• pp.227-233
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• 2014

The mixture (MX) of acetic acid (AA) and lactic acid (LA) was examined for its effectiveness in the control of the pine wood nematode Bursaphelenchus xylophilus contaminated in cymbidium culture medium. Nematode mortality in vitro was nearly 100% in AA and MX at the concentrations of 5.0-1.0% (pH 2.6 - 4.2) and in LA only at 5.0% (pH 3.5), but lowered at concentrations of 0.5-0.1% (pH 5.1-6.9) more significantly in LA than AA and MX. MX of most concentrations caused higher nematode mortality than the average response to AA and LA. All treatments of MX (0.5% and 0.25%), fosthiazate (standard and double concentrations) and culture dilution of Paenibacillus polymyxa GBR-1 ($10^7$colony-forming units/ml) reduced significantly the nematode populations in the cymbidium culture medium, compared to non-treatment control, with no significant difference among the treatments. No phytotoxicity occurred in all treatments. pH of the medium with the time after treatment and growths of 2-year-old cymbidium were not significantly different among treatments. Considering the safety and price of the organic acids, use of MX in the processes for culturing cymbidium may be a practically reliable and eco-friendly way in the control of the quarantine nematode in cymbidium.

• Journal of Animal Environmental Science
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• v.16 no.3
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• pp.215-226
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• 2010

The pig slurry leachate was dark brown-colored solution that leaches out of woodchip trickling filter. The purpose of this research was to investigate the effect of pig slurry leachate and byproduct on growth characteristics of leaf lettuce in hydroponics culture. The effects of addition of fish meal, bone meal and crab shell for the growth of leaf lettuce were investigated. Leaf lettuce were grown in each of six combination treatment solutions; slurry leachate, slurry leachate + fish meal, slurry leachate+bone meal, slurry leachate + crab shell and chemical hydroponic solution for lettuce based on EC content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of lettuce. The all of nutrient solution was adjusted 1.5 mS/cm in EC in hydroponics culture. 1. The pH level of leachate of trickling filter was increased and EC decreased gradually during treatment. Pig slurry leachate was low in suspended solids (SS), phosphorus (P), but rich in potassium (K). 2. The plot of slurry leachate (SL) was lowest in the growth characteristics of lettuce. The leaf length and width of lettuce treated with mixture plot of slurry leachate and fish meal (SL + FM) was higher compared with plot in slurry leachate. The chlorophyll reading was reduced in plot treated with slurry leachate, but that in plot of SL+FM was similar compared with control plot. 3. The fresh weight of lettuce showed lowest in the plot treated with slurry leachate. The addition of fish meal increased the yield of comparing plot of slurry leachate, but plots of bone meal and crab shell addition were not significantly difference. The fresh weight of leaf lettuce in plot of SL+FM was 87% as 400.0g compared with control. In conclusion, the mixture solution of pig slurry leachate and fish meal could be used as a nutrition solution of organic lettuce hydroponics.

• The Korean Journal of Pesticide Science
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• v.19 no.2
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• pp.141-150
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• 2015

Thirty-two actinomycetes isolates from Korean forest soil were screened for their nematicidal and reproduction suppression activity against pine wood nematode (PWN) which is widely spread in Korea. Culture filterates of 21 isolates showed more than 90% mortality at 2-fold concentration. Among them, AM210, SG16, YD116 and YD315 were more effective than others on reproduction of PWN. The YD116 isolate was identified as Streptomyces atratus by morphological and 16S rDNA analyses. Hydrazine hydrate, similar to hydrazidomycin which has cytotoxicity among substances from S. atratus against PWN, was tested for its nematicidal activity. Ten ppm of the hydrate showed 60.8% mortality. Additional studies are needed for practical use of the S. atratus YD116 isolate.

• Journal of Korean Library and Information Science Society
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• v.44 no.3
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• pp.447-470
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• 2013

The purpose of this study is to examine how the cultural aspects of the publication of literary collections have been changed from its time throughout the entire Joseon Dynasty. At the royal court in the early Joseon Dynasty, the need for publication of the literary collections has consistently arisen to preserve writings of the noted sages, and the Joseon government was also taking the lead and promoting the publication of the literary collections. From the 16th to the early 18th century, the publication was intensively made at the local governments led by local governors. From the 17th to the early to middle of the 18th century, the finances of local governments were limited with the changed taxation system, and there had been a dramatic decrease in the number of literary publication projects. On the other hand, with the sudden increase of the number of Seowons during the reign of King Sukjong, the focus of the literary publication was moved to Seowon from the 18th to the 19th century. After the enforcement of the Seowon Abolition Decree, the collections were still published at Seowons, however from the end of 19th century, the publication of literary collections had been explosively made by aristocrats who did not belong to the institution of Seowon or could not receive Seowon's support, which spreaded over the entire Yangban culture.

• The Korean Journal of Mycology
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• v.24 no.4 s.79
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• pp.246-254
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• 1996

Since the mid of 1980's, cultivation area and production of Ganoderma lucidum have been increased annually in Korea. However, the presence of a fungal disease has become a major limiting factor in the cultivation of Ganoderma lucidum, causing a serious economic loss. The present study was carried out to isolate and identify the pathogenic fungus to Ganoderma lucidum. Several fungi isolated from the wood logs showing typical symptoms were tested whether they are pathogenic to Ganoderma lucidum or not by cross-pairing culture method, flask inoculation method, and wood log inoculation method. The pathogenic fungus produced ascomata. Mature ascomata was spherical, dark, thick-walled, $45{\sim}95\;{\mu}m$ diameter. Asci were thin-walled, evanescent when mature, disintegrate early. Ascospores were spherical, hyaline, glaborous, thick-walled, refractive, $3.6{\sim}4.3\;{\mu}m$ in size. Conidiophores soon became abundantly septate and broke up into arthrospores, which are cylindrical, $3{\sim}6\;{\mu}m$ long and $3{\sim}4\;{\mu}m$ wide. Based on the observations under dissecting microscope, light microscope and scanning electron microscope, teleomorph and anamorph of the pathogenic fungus were identified as Xylogone sphaerospora Von Arx & Nilsson and Sporendonema purpurascens (Bonordon) Mason & Hughes, respectively. X. sphaerospora is first reported as a pathogenic fungus of Ganoderma lucidum.

• Journal of Korean Society of Forest Science
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• v.65 no.1
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• pp.107-112
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• 1984

Theoretical studies on the effects of forest road are considered to be significant from a present viewpoints which is headed for mordernization and activation of forestry. 1) From a viewpoint of wood production techniques, forest road is enable to economical transport of all kinds of forest products and easy transport to forest of operative tools and necessary materials, and largely contribute to efficiency of all operations, including reforestation, tending, forest protection and conservation of forests. 2) And, from a viewpoint of forest management, development of forest road makes wood marketability be secured and curtailment of various indirect expenditure with the operation efficiency be possible. 3) Forest road, also in living aspects of inhabitants in mountainous villages, plays direct and indirect roles in promoting their welfare including improvement of employment for forest labor, traffic benefits in mountainous area and introduction of culture. Therefore, as a premise to realize intensive management in forestry, the necessities of forest road were increased in its aspects of important and fundamental facilities as well as production basis.

• Journal of the Korean Wood Science and Technology
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• v.35 no.6
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• pp.166-174
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• 2007

To know the effect of enzymatic pre-treatment on softwood Kraft pulp, two xylanse-encoding genes, named xynl and xynll were isolated from Thrichoderma ressei. Structural genes of xylanase (XYNI, XYNII) and cellulase (EGIV-CBDII) were isolated from T. ressei and Rumicoccus albus respectively, and expressed in E. coli. bacterial culture. The specific activity of purified recombinant XYNI is higher than XYNII. The brightness of XYNI treated softwood Kraft pulp increased to 29.9%. On further sequential treatment with EGIV-CBDII and XYNI the brightness of softwood Kraft pulp were improved to 9.1 and 73% respectively. As expected the Kappa number of softwood Kraft pulp also decreased 8.1, 4.6 and 3.2% respectively. Results further indicate that this sequential combination of enzyme treatment has synergic effect for improving bleaching of softwood Kraft pulp.

• Journal of the Korean Wood Science and Technology
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• v.37 no.6
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• pp.585-599
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• 2009

This study was carried out to investigate the antifungal activity of the water soluble essential oil against Trichoderma spp. Water soluble essential oils from Pinus densiflora and Chamaecyparis obtusa were obtained from GAP (Gas assisted process) extraction apparatus. When the water soluble essential oil was treated, the growth of the conidial germination was maximum under the cultivation condition at $25^{\circ}C$ and in the culture medium adjusted to pH 5.0. The yield of water soluble essential oil was 3.9% and 3.7% in P. densiflora and C. obtusa, respectively. 24 and 15 kinds of compounds were identified in water soluble essential oils of P. densiflora and C. obtusa, respectively. The major components in the essential oil of P. densiflora were $\alpha$-Terpineol acetate and Terpinen-4-ol and those of C. obtusa were Terpinen-4-ol and $\alpha$-Terpineol. Antifungal activity was the best in the 5000 ppm of P. densiflora against Trichoderma harzianum and in 5000 ppm of C. obtusa against Trichoderma atroviride.