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Biobleaching of Softwood Kraft Pulp Using Recombinant Xylanase and Cellulase  

Kim, Hyun Joo (BK21 : Added-value utilization of lignocellulosic biomass, College of Agriculture & Life Science, Chonnam National University)
Wi, Seung Gon (BK21 : Added-value utilization of lignocellulosic biomass, College of Agriculture & Life Science, Chonnam National University)
Bae, Hyeun-Jong (BK21 : Added-value utilization of lignocellulosic biomass, College of Agriculture & Life Science, Chonnam National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.35, no.6, 2007 , pp. 166-174 More about this Journal
Abstract
To know the effect of enzymatic pre-treatment on softwood Kraft pulp, two xylanse-encoding genes, named xynl and xynll were isolated from Thrichoderma ressei. Structural genes of xylanase (XYNI, XYNII) and cellulase (EGIV-CBDII) were isolated from T. ressei and Rumicoccus albus respectively, and expressed in E. coli. bacterial culture. The specific activity of purified recombinant XYNI is higher than XYNII. The brightness of XYNI treated softwood Kraft pulp increased to 29.9%. On further sequential treatment with EGIV-CBDII and XYNI the brightness of softwood Kraft pulp were improved to 9.1 and 73% respectively. As expected the Kappa number of softwood Kraft pulp also decreased 8.1, 4.6 and 3.2% respectively. Results further indicate that this sequential combination of enzyme treatment has synergic effect for improving bleaching of softwood Kraft pulp.
Keywords
biobleaching; cellulase; softwood Kraft pulp; recombinant protein; xylanase;
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