• 산업융합연구
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• 제18권6호
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• pp.51-59
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• 2020

본 연구는 간호대학생을 대상으로 웰다잉에 대한 인식과 연명치료중단의 태도 및 안락사에 대한 태도 간의 관계를 알아보고자 시도되었다. 연구 기간은 2018년 4월 1일부터 5월 6일까지로 D광역시에 소재한 간호학과의 288명의 간호대학생을 대상으로 조사를 실시하였다. 연구결과 웰다잉에 대한 인식은 연명치료중단의 태도와 적극적 안락사, 소극적 안락사와 정(+)의 상관관계로, 연명치료중단의 태도는 적극적 안락사, 소극적 안락사와 정(+)의 상관관계로 나타났다. 간호사는 연명치료과정에서 많은 윤리적 갈등을 경험할 것으로 예상되는 바 간호대학생 시기에 웰다잉에 대한 인식, 생명윤리교육을 받는 것이 필요하고 교육프로그램의 개발과 효과평가가 요구된다.

• Fisheries and Aquatic Sciences
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• 제22권12호
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• pp.29.1-29.7
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• 2019

Veterinary drugs are widely used to protect production-related diseases and promote the growth of farmed fish. The use of large amounts of veterinary drugs may have potential risk and cause adverse effects on both humans and the environment. In this study, we developed risk-based ranking based on a scoring system to be applied in the national residue program. In this approach, the following three factors of veterinary drugs that may occur as residues in fishery products were considered: potency (acceptable daily intake), usage (number of dose and withdrawal period), and residue occurrence. The overall ranking score was calculated using the following equation: potency × usage (sum of the number of sales and withdrawal period) × residue occurrence. The veterinary drugs that were assigned high score by applying this approach were enrofloxacin, amoxicillin, oxolinic acid, erythromycin, and trimethoprim. The risk-based approach for monitoring veterinary drugs can provide a reliable inspection priority in fishery products. The developed ranking system can be applied in web-based systems and residuemonitoring programs and to ensure safe management of fishery products in Korea.

• 대한수의학회지
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• 제46권3호
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• pp.291-294
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• 2006

$Parallux^{TM}$, a solid-phase fluorescence immunoassay(SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of fish muscle. The recommended therapeutic dose of ampicillin(100 mg/kg body weight, withdrawal period 7 days) was orally administered to a group of 25 olive flounders(Paralichthys olivaceus) for consecutive five days. Muscle was sampled after drug treatment 1st, 2nd, 3rd, 4th and 5th day. The concentration of ampicillin in muscle, determined by SPFIA, was compared with that of internal standard(10 ppb as ampicillin). The absorbance ratio of sample to internal standard(Bs/Bo) was employed as an index to determine the muscle residue in olive flounder. To investigate the recovery rate, the standard solutions were added to muscle samples to give final concentrations in muscle of 10 and 50 ng/ml. The recovery rates of all spiked samples were > 89% of the spiked value. Ampicillin was detected in muscle of fishes treated until the 3rd day of withdrawal period. The present study showed that the SPFIA can be easily adopted in predicting tissue residues for ampicillin in farmed fishes.

• Journal of Preventive Veterinary Medicine
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• 제42권4호
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• pp.171-176
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• 2018

A study of the tissue depletion of florfenicol (FFC) administered orally to pigs at a dose of 0.05 kg/ton feed for 7 days was performed. Sixteen healthy cross swine were administered with FFC. Four treated animals were arbitrarily selected to be sacrificed 1, 3 and 5 days after the end of treatment. FFC residue concentrations in muscle, liver, kidney, and fat were determined using high-performance liquid chromatography (HPLC) with ultraviolet photometric detector at 230 nm. The correlation coefficient ($R^2$) of the calibration curve for florfenicol amine (FFCa) was > 0.997 and the limits of detection and quantification were 0.012 and $0.040{\mu}g/mL$, respectively. Recovery rates in swine edible tissues ranged from 79.1 to 93.5%. In the FFC-treated group, FFC residues at 3 days post-treatment were below the maximum residue limits (MRLs) in muscle, kidney and fat, and those at 5 days post-administration were below the MRLs in all edible tissues. These results suggest that the withdrawal period of FFC after the drug treatment might be 5 days, which is a sufficient amount of time for reduction of the FFC residues below the MRLs in all edible tissues.

• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.241-248
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• 2005

This study is aimed at understanding the role of arsenic in Roxarsone in causing fatty livers in mule ducks. One hundred 10-week-old mule ducks were randomly divided into 5 groups. Ducks received 2 weeks of various treatments followed by 2 weeks of withdrawal. The treatments were non-treatment (control), 300 mg/kg Roxarsone inclusion for 2 weeks ($1^{st}$ and $2^{nd}$ week), Roxarsone inclusion for one week ($2^{nd}$ week only), restrict feeding, or Roxarsone analogue (3-nitro-4-hydroxyphenyl acid) inclusion. Results showed that feed intake and body weight in the Roxarsone groups and the restrict feeding group decreased significantly during the treatment period. However only the liver and heart weights were significantly decreased (p<0.05) in the restrict feeding group. Fatty acid synthetase (FAS) activity showed a significant decrease (p<0.05) in the Roxarsone groups and the restrict feeding group, two-week-Roxarsone treatment significantly increased NADP-malic dehydrogenase (MDH) activity compared to the restrict (p<0.05). After 2 weeks drug withdrawal, the 1-week-Roxarsone or restrict feeding group showed significantly increased (p<0.05) glucose-6-phosphate dehydrogenase (G-6-PDH) activity (p<0.05). Two-week-Roxarsone treatment significantly decreased (p<0.05) the high density lipoprotein (HDL) and increased (p<0.05) the low density lipoprotein (LDL) and very low density lipoprotein (VLDL) ratio. After drug withdrawal, the 1-week-Roxarsone or restrict feeding group showed significantly increased (p<0.05) creatine kinase (CK) activity. The 2-week-Roxarsone treatment group showed significantly increased (p<0.05) aspartate aminotransferase (AST) activity. The restrict feeding treatment group showed significantly decreased (p<0.05) total protein (TP) concentration. After drug withdrawal, the related enzyme activities in the blood that reflected the liver function were restored to the normal physiological range, except for the total bilirubin concentration and CK activity in the 1-week-Roxarsone group. This group showed a significant increase (p<0.05). Thus, the reasons for liver enlargement in the Roxarsone and restrict feeding groups were different.

• 한국수산과학회지
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• 제42권3호
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• pp.204-208
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• 2009

The withdrawal time of erythromycin in cultured olive flounder was investigated to ensure the food safety of the fish treated with erythromycin. The oral administration of erythromycin was carried out using the experimental diet containing erythromycin (200 mg/kg) daily dose of 40 mg/kg body weight. The 45 day experimental period was broken into 7 days of habituation, 8 days of medication and 30 days of additional feeding without antibiotics. The erythromycin concentration in the flounder muscle had been increased gradually with medication. After 5 days of medication, the concentration increased to its maximum level of 6.05 mg/kg. After discontinuing the antibiotic, the erythromycin concentration decreased drastically and day 9 was below 0.1 mg/kg. The erythromycin concentration had slowly declined from the 6th to the 20th day after medication and disappeared completely after 25 days. From these results, the time needed to reduce the erythromycin level to the 0.2 mg/kg limit adopted by the ED and Japan was suspected to be 4-6 days. Therefore, a reasonable withdrawal time following ED and Japanese regulatory guidelines for erythromycin in the cultured flounder could be estimated to be 10 days.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1247-1264
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• 2021

Anabolic steroids are frequently used to increase the growth rate of meat-producing animals. Exposure to an anabolic-androgenic steroid, nandrolone decanoate (ND), is associated with expressional reduction of testicular steroidogenic enzymes. However, the effect of withdrawal of ND exposure on the expression of these testicular molecules has not been thoroughly explored. The current research investigated expression changes of testicular steroidogenic enzymes in rats at several recovery periods (2, 6, and 12 weeks) after the stop of ND treatment with different doses (2 and 10 mg/kg body weight) for 12 weeks. Body and testis weights were recorded, and transcript levels of molecules were determined by quantitative real-time polymerase chain reaction (PCR). The immunohistochemistry was used to examine the changes of immuno-intensities of molecules. At 6 and 12 weeks of the recovery period, the 10 mg/kg ND-treated rats were lighter than other experimental groups. The interstitial compartment vanished by ND treatment filled up as the recovery period became longer. The expression of steroidogenic acute regulatory protein was returned to the control level at 12 weeks of the recovery period. Expression levels of cytochrome P450 side-chain cleavage and 17a-hydroxylase were increased in 2 mg/kg ND-treated group at 6 weeks of the recovery period, and transcript levels of these molecules in 2 and 10 mg/kg ND-treated groups at 12 weeks of the recovery period were significantly lower than the control. Expression levels of 3β-hydroxysteroid dehydrogenase (HSD) type I and 17β-HSD type 3 in 2 mg/kg ND-treated group were comparable with those of control at 12 weeks of the recovery period, but not in 10 mg/kg ND-treated group. Expression of cytochrome P450 aromatase (Cyp19) was reverted to the control level at 2 weeks of the recovery period. Except for Cyp19, there was a visible increase of immuno-staining intensity of other testicular steroidogenic enzymes in the Leydig cells as the recovery period progressed. This research has demonstrated that the cease of ND administration could restore the expression of testicular steroidogenic enzymes close to the normal level. Nevertheless, a relatively long recovery period, compared to the ND-exposure period would be required to retrieve normal expression levels of testicular steroidogenic enzymes.

• Asian-Australasian Journal of Animal Sciences
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• 제4권3호
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• pp.275-280
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• 1991

Beta-aminopropionitrile (BAPN)-a Lathyrus toxin was fed to laying chicken to investigate effects on egg weight, production and malformity. Two feeding trials were conducted where the levels of BAPN varied from 0.1 to 0.6 g per kg diet. The responses with regard to egg weight, egg production and the incidence of malformed eggs due to varying concentrations of the toxin were recorded and the results on weekly changes in these important variables were evaluated by plotting graphs. The results showed that there were increasing trends in egg weight and egg shell abnormalities and a decreasing trend in egg production as the dietary level of BAPN was increased indicating that the responses of layers were dose dependent. The effects on egg weight, egg production and egg malformity disappeared following withdrawal of the toxin from the diets.

• 한국가금학회지
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• 제29권1호
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• pp.37-43
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• 2002

본 시험은 왕겨에 의한 사료의 희석과 무급여일 설정방법에 의한 조기제한사양이 육계의 보상성장과 사료효율 및 체지방 축적에 미치는 영향을 구명하기 위해 7일령된 무감별 육계 300수를 5처리 3반복, 반복당 20수씩 공시하여 7일령에서부터 49일령까지 실시하였다. 대조구(C)는 7일령부터 49일령까지 전기간 육계 전기사료와 후기사료를 자유채식을 시켰으며, 시험구(71-74)들은 모두 7일령부터 14일령까지 육계전기사료와 왕겨를 50:50(wt/wt)으로 혼합한 희석사료를 급여하였으며, 제한사양기간동안 T1은 희석사료를 자유채식시켰으며, T2는 1일절식-3일급여, T3는 1일절식∼2일급여 및 T4는 1일절식∼1일급여하였고, 제한 사양이 끝난 시험구들은 전기사료 및 후기사료를 자유채식시켰다. 제한사양기간을 포함한 21일령까지의 증체량과 21일령 체중 모두 T1은 대조구와 유의적 차이가 없었으나 다른 시험구들은 대조구보다 적었다(P<0.05). 22일령부터 49일령가지의 증체량은 T1구가 대조구와 유의적인 차이는 없었으나 가장 높은 증체를 보였다. 전기간인 7일령부터 49일령 까지의 증체량과 49일령 체중도 T1구만 대조구와 유의적인 차이가 없었고 다른 시험구들은 대조구보다 적었다(P<0.05) .제한사양후부터 21일령까지의 사료섭취량은 T1구를 제외한 나머지 시험구에서는 대조구에 비해 유의적으로 적었으며 (P<0.05) , 전기간 셥취량도 T1구만 대조구와 유의적인 차이를 보이지 않았으나, 희석사료섭취기간의 왕겨섭취량을 제외한 육계사료 섭취량에서는 모든 시험구들이 대조구에 비해 유의적으로 적게 섭취하였다(P<0.05) 제한사양기간을 포함한 7일령부터 21일령까지의 사료요구율은 T1구와 T2구가 대조구에 비해 유의적으로 높았으며(P<0.05), 22일령부터 49일령가지의 사료요구율은 대조구에 비해 모든 시험구들이 유의적으로 낮았으며(P<0.05), 전기간동안의 사료요구율은 T1구를 제외한 나머지 시험구들은 대조구에 비해서 유의적으로 낮았으나(P<0.05) , 희석사료섭취기간의 왕겨섭취 량을 제외 한 육계 사료요구율은 대조구에 비해서 모든 시험구들이 유의적으로 낮거나(P<0. 05) 낮아지는 경향을 보였다. 복강내 지방함량 및 전기간 폐사율은 처리간에 유의적 차이가 없었다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.455-458
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• 2004

This study uses time series analyses to evaluate fluctuation of water levels in a geothermal water well due to pumping, in relation to rainfall at Dongrae hot-spring site on the southeastern coast of tile Korean peninsula. The volume of water pumped from the public study wells ranges from 542 to 993 m$^3$/month, and the minimum water level ranged from 35 to 144.7 m during the measured period. Autocorrelation analysis was conducted for the withdrawal rate at the public wells, water levels and rainfall. The autocorrelation of the withdrawal rate shows distinct periodicity with 3 months of lag time, the autocorrelation of rainfall shows weak linearity and short memory with 1 months of lag time, and the autocorrelation of water levels shows weak linearity and short memory with 2 months of lag time. The cross-correlation between the pumping volume and the minimum water level shows a maximum value 1 at a delayed time of 34 months. The cross-correlation between rainfall and the minimum water level shows a maximum value of 0.39 at a delayed time of 32 months.