Journal of Preventive Veterinary Medicine

The Korean Society of Preventive Veterinary Medicine (한국예방수의학회)
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Determination of florfenicol residues in swine tissues using high-performance liquid chromatography with ultraviolet photometric detector

  • Chae, Won-Seok (Schools of Life-Chemistry, Daejin University) ;
  • Yoo, Chang-Yeul (Department of Computer Software Science, Gyeongnam Provincial Namhae College) ;
  • Tutkun, Lutfiye (Department of Medical Biochemistry, Bozok University) ;
  • Kim, Suk (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2018.11.02
  • Accepted : 2018.12.22
  • Published : 2018.12.31
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Abstract

A study of the tissue depletion of florfenicol (FFC) administered orally to pigs at a dose of 0.05 kg/ton feed for 7 days was performed. Sixteen healthy cross swine were administered with FFC. Four treated animals were arbitrarily selected to be sacrificed 1, 3 and 5 days after the end of treatment. FFC residue concentrations in muscle, liver, kidney, and fat were determined using high-performance liquid chromatography (HPLC) with ultraviolet photometric detector at 230 nm. The correlation coefficient ($R^2$) of the calibration curve for florfenicol amine (FFCa) was > 0.997 and the limits of detection and quantification were 0.012 and $0.040{\mu}g/mL$, respectively. Recovery rates in swine edible tissues ranged from 79.1 to 93.5%. In the FFC-treated group, FFC residues at 3 days post-treatment were below the maximum residue limits (MRLs) in muscle, kidney and fat, and those at 5 days post-administration were below the MRLs in all edible tissues. These results suggest that the withdrawal period of FFC after the drug treatment might be 5 days, which is a sufficient amount of time for reduction of the FFC residues below the MRLs in all edible tissues.

Keywords

Acknowledgement

Supported by : Dae Han New Pham Co. Ltd.

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