• The Plant Pathology Journal
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• 제35권6호
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• pp.553-563
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• 2019

Investigations related with factors influencing root and crown rot are rare and mainly related to farming practice and soil management. The main objective of this study was to examine broader range of factors influencing stem-base infestation of winter wheat in the field conditions. The effect of spatial distribution of infected plants on disease index (DIs) assessments was also investigated. Analysis of factors influencing DIs of crown rot of wheat demonstrated significant influence of the growing seasons (P < 0.001) and extreme fluctuations in winter temperatures (P < 0.001). In addition to that, localities together with their interaction with the growing season also significantly influenced DIs (P < 0.001). Aggregation of infected plants influenced variability of DI estimations, and it was pointed out that more extensive investigation should be conducted on broad range of DI in order to establish sampling method giving uniform sampling precision. Fusarium graminearum was shown to be predominant Fusarium species in Serbia (72.6%) using sequence-characterized amplified region analysis. Interestingly F. oxysporum was isolated in higher frequencies (27.4%) than it was reported in the literature. Given that there were no reports on the diversity of Fusarium species causing crown rot of wheat in Serbia, this study presents first report on this important subject. It also indicated that more attention should be focused on combined effects of abiotic and biotic factors influencing stem-base infestation of winter wheat. This knowledge will contribute to better understanding of factors influencing root and crown rot of wheat which would ensure sustainable disease management in the future.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 추계 학술대회지
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• pp.30-31
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• 1990

• 식물병연구
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• 제14권3호
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• pp.153-158
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• 2008

유묘를 이용한 보리와 밀의 흰가루병에 대한 실내 대량 검정 방법을 개발하고 국내 육성 품종 및 유전자원에 대해 저항성 정도를 검정하였다. 종이 봉투를 이용한 유묘 검정은 파종에서 접종이 가능한 생육까지 약 10일이 소요되었으며, 접종 후 발병 및 검정까지는 약 8일로 3주 이내에 저항성 정도를 확인할 수 있었다. 이 방법은 1회에 180종 이상을 검정할 수 있다. 유묘 검정시 저항성 반응은 포장과 마찬가지로 무감염과 괴사증상 두가지 반응을 보여 이를 이용하여 저항성 정도를 판정할 수 있었다. 국내 육성 보리 79품종 중 겉보리에서는 '상록보리'만이 저항성이었고, 맥주보리에서는 '다진보리'가 저항성을 보였고 '제주보리'는 중도 저항성을 나타내었다. 쌀보리와 밀 25품종은 모두 감수성을 의였다. 이 결과는 포장에서의 저항성성과 같은 결과를 보여 맥류의 흰가루병 저항성 검정에 이용하는 것이 가능하였다. 한편 국내에서 보리와 밀의 품종육성에 이용되는 유전자원 검정 결과 맥주보리에서 가장 많은 51종이 저항성 자원으로 선발되었다. 그러나 겉보리와 쌀보리 796종과 밀 273종에서는 저항성 자원이 확인되지 않았다.

• 한국작물학회지
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• 제50권5호
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• pp.346-355
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• 2005

To investigate the application of biochemical markers' and small-sample methods using whole-wheat flours for screening in early generation in Korean wheat breeding system, 74 Korean wheats, including cultivars, local breeding lines and experimental lines, were analyzed. Seed storage protein and amylose contents of grains were evaluated. Biochemical makers, including granule bound starch synthase (GBSS), high molecular weigh glutenin subunits (HMW-GS) and friabilin were also evaluated by using one-dimensional sodium dodecyl sulfate-polyacryla-mide gel electrophoresis with a single kernel. The small­sample methods, including modified SDS-sedimentation test (MST), micro-alkaline water retention capacity (AWRC) and whole-wheat flour swelling volume (WSV) were also tested in this study. Protein content, MST and AWRC was $11.0 - 15.8\%$, 2.7 - 26.2 ml and $71.9 - 109.7\%$, respectively. Apparent and total amylose content and WSV was $20.6 - 25.0\%$, $26.1 - 32.4\%$ and 9.0 - 16.9 ml, respectively. There were highly significant correlations between MST and AWRC (r=0.592, P<0.001), but Korean wheats showed no significant difference in protein content, amylose content and small-sample methods. In the biochemical markers, Korean wheats contained all three GBSS encoded by Wx loci, except for Suwon 252. Korean wheats showed the high frequency ($58.1\%$) of 1Dx2.2 + 1Dy12 subunits of HMW-GS. Friabilin band was present in 46 lines ($62.2\%$) and absent in 28 lines ($37.8\%$). Friabilin-absence lines showed the higher MST (14.9 ml) and AWRC ($92.1\%$) value than friabilin-presence lines (8.5 ml and $82.4\%$, respectively).

• Journal of Plant Biotechnology
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• 제1권1호
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• pp.8-12
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• 1999

New winter wheat winter barley hybrids were produced (Mv9 kr1 Igri, Mv9 kr1 Osnova, Asakazekomugi Manas). The wheat-barley hybrids showed entire male sterility and were multiplied in tissue culture. Chromosome configurations were studied with GISH in meiosis in the Mv9 krl x Igri hybrid and in its progenies multiplied in vitro. Chromosome pairing between wheat and barley has been observed in some cells in the hybrids multiplied in vitro. Backcross plants with 43 and 44 chromosomes were selected with the aim of developing new wheat-barley addition lines. Wheat-barley translocations were demonstrated with GISH in backcross progenies originating from in vitro regenerated wheat (Triticum aestivum L. cv. Chinese Spring) x barley (Hordeum vulgare L. cv. Betzes) hybrids. Five different translocations were observed. Sequential N-banding and GISH analyses were performed to further identify the translocations. The N-banding pattern of the Robertsonian translocation suggests that this chromosome consists of the short arm of barley chromosome 4H translocated to the long arm of 2B of wheat. Plants with four different homozygous translocations were selected from the following BC2F3 generation.

• 한국농림기상학회지
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• 제18권3호
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• pp.99-108
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• 2016

최근 겨울 기온이 상승하면서 밀의 파종시기가 변화되고 있다. 겨울 밀의 경우, 파성 소거에 필요한 저온 노출지속기간이 충족되지 않으면 출수뿐만 아니라 생육이 불안정해질 수 있다. 한국의 농촌진흥청 국립식량과학원은 국제옥수수밀 연구소의 멕시코 톨루카에서 한국의 기후에 맞는 밀 육종을 위해 1996년 이후 지속적으로 밀 적응시험을 수행해 왔다. 그러나 톨루카의 최근 겨울기후가 상승하는 등 이상기후 발생에 의해 밀 파종시기 및 생육에 변화가 발생하였고 이에 따라 우리나라 겨울 밀의 육종 및 적응시험 전략의 변화가 필요하여 이를 위한 기초자료를 제공하고자 본 연구를 수행하였다. 2013년과 2014년에 파종된 CIMMYT 교배 품종과 우리나라 겨울 밀 교배 품종의 출수생태를 그룹별(II and III) 저온 노출지속기간의 변화와 결합하여 파종시기의 변화에 따른 저온 노출지속기간과 출수율에 미치는 영향을 분석하였다. 2013년 11월 6일, 22일, 12월 6일로 3회 파종시기에서 11월 6일에 파종한 것은 저온 노출지속기간은 충분히 충족되지 않았고, 14일 이후인 11월 22일과 12월 6일에는 저온 노출지속기간이 충족되었다. 그러나 2014년 11월 5일과 20일에 파종한 경우는 저온 노출지속기간이 충족되었고, 오히려 12월 5일에 파종한 것에서 저온 노출지속기간이 충족되지 않았다. 이것은 2012년 이후 11월에 빈번한 고온 발생에 의해 11월 파종 후 저온 노출지속기간을 충족시키지 못했던 반면, 2014년 11월 일별 최저기온은 $3.0^{\circ}C$ 미만으로, 11월 파종 후에도 저온 노출지속기간을 충족할 수 있는 기후환경이 유지되었기 때문인 것으로 판단된다. 또한, 우리나라 겨울 밀의 품종 개량 및 적응시험에서 춘파성인 그룹 II는 3회 파종시기 변경에 따라 출수율의 증감 차이가 확실하지 않았는데, 추파성인 그룹 III는 파종시기별 출수율이 뚜렷하게 나타나서 추파성인 그룹 III가 파종시기 이동에 더 민감할 것으로 판단되어 shuttle breeding의 효과가 더 클 것으로 보인다. 또한, 가까운 미래 기후변화 시나리오 전망에 적용한 결과, 톨루카에서 그룹 II의 미래 적정 파종시기는 예측되었지만, 그룹 III에서는 미래 적정 파종시기를 아예 예측할 수 없었기 때문에 추파성인 그룹 III에 대한 shuttle breeding 에는 이러한 점이 고려되어야 할 것으로 판단된다.

• 한국작물학회지
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• 제47권1호
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• pp.13-20
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• 2002

Bread baking parameters and relationships between bread baking properties and flour characteristics were evaluated for two years, 1997 and 1998, and at two locations, Suwon and Deokso, with Korean winter wheat cultivars and lines. Among the bread baking parameters, lightness of crumb grain showed differences between years. No significant differences were found in dough mixing time, bread loaf volume, crumb grain score or firmness. Keumkangmil, Suwon 278 and Tapdongmil showed higher bread loaf volume, good structure of crumb grain and softer crumb firmness. However, compared to commercial flours for baking, cultivar means averaged over years and locations of nineteen Korean winter wheats showed poor bread baking quality because of low protein content and unsuitable protein quality. Protein content and flour swelling volume showed better relationships with the bread baking parameters than other flour characteristics. Friabilin-absence lines showed softer crumb firmness than those of friabilin-presence lines.

• 한국작물학회지
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• 제47권1호
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• pp.21-28
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• 2002

Noodle texture parameters of Korean style wet and dry noodles and relationships between noodle quality and flour characteristics were evaluated for two years, 1997 and 1998, and at two locations, Suwon and Deokso, using Korean winter wheat cultivars and lines. No significant difference for chewiness was found between cultivars over locations. Noodles made from flours from 1997 showed significantly higher chewiness than those from 1998. Chewiness of cooked noodles showed positively significant correlations with protein content and SDS sedimentation volume and negatively significant correlations with starch peak viscosity and flour swelling volume. Korean winter wheat cultivars, except for Gobunmil, Keumkangmil Tapdongmil, Suwon 265 and Suwon 280, showed chewiness of cooked noodles similar to commercial flours used for noodle making in Korea and Japan.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.20-20
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• 2022

A traditional wheat breeding program needs more than 12-13 years to develop a new cultivar. In recent years, 'Speed breeding (SB)' system, which uses extremely extended photoperiod (22 h), enabled up to 4-6 generations of spring wheat per year. However, since almost 70% of wheat cultivars are winter type, and over 95% of total cultivation area is for winter wheat in Korea, optimized vernalization treatment was essential for improving the SB system. Several vernalization temperatures and durations were tested with various genotypes, and the 4 weeks of 8-10 ℃ vernalization treatment was the most effective to develop 4 generations per year, for both spring and winter type wheat cultivars. This 'Speed vernalization (SV)' system followed by SB, allowed developing a new F₆ recombinant inbred lines (RILs) within 2 years. Among the 184 RILs, which derived from a cross between Jokoyung and Joongmo2008, two outstanding lines were selected for yield trial test, and then named Milyang52 and Milyang53. Compared to the traditional wheat breeding program, over 60% of the time was saved to develop these two lines. Marker-assisted selection and backcross were also combined with the SV system. YW3215-2B-1 (Jokoyung*3/Gamet), which has similar agronomic traits with Jokyoung and the same Glu-B1 allele with Garnet, was developed within 2.5 years. Thus, the SV system combined with molecular breeding technology would help breeders to make a new cultivar with less time and high efficiency.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 춘계 학술대회지
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• pp.82-83
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• 1990