• Title/Summary/Keyword: wild soybean

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Molecular and Biological Characterization of an Isolate of Cucumber mosaic virus from Glycine soja by Generating its Infectious Full-genome cDNA Clones

  • Phan, Mi Sa Vo;Seo, Jang-Kyun;Choi, Hong-Soo;Lee, Su-Heon;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.30 no.2
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    • pp.159-167
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    • 2014
  • Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.

Variation of Seed Component Contents in Wild Soybean(Glycine soja Sieb. & Zucc.) (야생콩(Glycine soja Sieb. & Zucc.)의 종실성분 함량변이)

  • Yun Hong-Tae;Seo Min-Jung;Kim Sun-Lim;An Sun-Ok;Kim Si-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.spc1
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    • pp.108-111
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    • 2005
  • Studies were conducted to develop new breeding resources using wild soybean (Glycine soja Sieb. & Zucc.) species. In 132 wild soybeans, the average crude protein content was $45.4\%$, ranged from $37.4\%\;to\;50.2\%$ and average crude oil content was $9.3\%$ showing lower than cultivated soybean. The average total amino-acids content was $19,214\;{\mu}g/g$, containing $252\;{\mu}g/g$ of methionine and $103\;{\mu}g/g$ of cysteine, a sulfur containing amino-acid. In ratio of individual amino-acids, the glutamic acid, methionine and cystein content was $16.1\%,\;1.3\%,\;and\;0.5\%$, respectively. The correlation between total amino-acid and sulfur containing amino-acid was not significant. Total isoflavone concentration was, on average, $1,105\;{\mu}g/g$ in wild soybean germplasm. As a result of comparison with total isoflavone and genestein contents, significant positive correlations were observed between total isoflavone and genistein concentration.

Studies on electrophoretic pattern and amino acids of wild soybean protein[1] -Acrylamide gel electrophoretic pattern of seed protein- (야생대두단백질(野生大豆蛋白質)의 전기영동유형(電氣泳動類型)및 아미노산조성(酸組成)에 관(關)한 연구(硏究)[1] -종실단백질(種實蛋白質)의 Acrylamide gel 전기(電氣) 영동유형(泳動類型)-)

  • Park, H.;Lee, J.S.;Lee, C.Y.
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.247-254
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    • 1977
  • Acrylamide gel electrophoretic pattern of 13 wild soybean (Glycine ussuriensis) was compared with that of G. gracilis and G. max var. Gwanggyo. Average Protein content (50%) of wild soybean was greater than that of C. gracilis (46%) and Gwanggyo (45%). Grain weight of wild soybean was one third of G. gracilis and one ninth of Gwanggyo. Electrophoresis of wild soybean protein showed total 16 different bands and three of which (Rm 0.09, 0.59 and 0.84) were specific and did not appeared in 86 var. of G. max which showed four specific bands (Rm 0.35, 0.45, 0.50 and 0.77) of total 17 bands. G. glacilis had all bands of Gwanggyo and two bands (Rm 0.53 and 0.59), one of which (Rm 0.59) was specific for wild soybean indicating that G. gracilis is middle type. Of 16 protein bands the third band (32%), the first band (28%) and the 5th band (13%) were main bands. Electrophoretic pattern could be sorted qualitatively into 4 groups, semiquantitatively into 6 groups and 2 or 4 groups depending on reference pattern by correlation or pattern similarity method. All sorting methods separated a wild soybean from Sogri mountain into a group and except that there were no similarity among methods but correlation methods seems more reasonable. Protein content was no relation with electrophoretic pattern but positively correlated with percent contribution of first band at 5% level suggesting that the first band may have a important role for protein synthesis.

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The Study on the Origin of Soybean Cultivation (대두재배(大豆栽培)의 기원(起源)에 관한 고찰(考察))

  • Lee, Sung-Woo
    • Journal of the Korean Society of Food Culture
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    • v.3 no.1
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    • pp.1-5
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    • 1988
  • According to the literature, soybean cultivation originated from Wang-Gong (B.C. 685-643) of China who brought it from northeast Asia, for the first time. FUKUDA, of Japan divided soybean into three species-the wild, the cultivated and the intermediate. From the result of that study, he concluded that the soybean originated in northeast Asia. But Wang Kum Rung of China insisted that soybean originated in Hwa-Nam, because the soybean is a shortday plant and the agricultural history of Hwa-Nam, south of China, is older than that of northeast Asia. However, agriculture in northeast Asia had been already begun about B.C. 4000-6000 and the origin of culture cannot be decided only by photosensitivity. It has been proved that soybeans found in Korea were same as the ones of B.C. 2000. The soybeans of northeast Asia meet the conditions of the probable place of origin of cultivated crops established by Vabilov. Accordingly it is concluded that soybean has been originated from northeast Asia.

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Positional mapping for foxglove aphid resistance with 180k SNP array in soybean [Glycine max (L.) Merr.]

  • Park, Sumin;Kim, Kyung Hye;Go, Hong Min;Lee, Ju Seok;Jung, Jin Kyo;Bilyeu, Kristin D.;Lee, Jeong-Dong;Kan, Sungtaeg
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.145-145
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    • 2017
  • Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The objective of this study was to identify the putative genes to foxglove aphid resistance in wild soybean, PI 366121 (Glycine soja Sieb. and Zucc.). One hundred and forty-one F4:8 recombinant inbred lines developed from a cross between susceptible variety, Williams 82 and foxglove aphid resistance wild soybean, PI 366121 were used. The two type of resistance response, antibiosis and antixenosis resistance were evaluated through choice and no-choice test, graded by the degree of total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 29,898 single-nucleotide polymorphism markers utilizing a Axiom(R) 180K soyaSNP array. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 was identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. The newly identified major QTL was consistent with previously reported QTL, Raso2, which showed around 5 times narrow down interval range with 8 candidate genes. Furthermore, total 1,115 soybean varieties including Glycine soja and Glycine max were exposed to germplasm screening, and 31 varieties, which showed significant antibiosis type foxglove aphid resistance were identified. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars and developing novel insecticides.

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Evaluation of Disease Occurrence by Cultivar, Sowing Date and Locational Difference in Korean Soybean Fields (콩의 품종, 파종시기 및 지역적 차이에 대한 병 발생 평가)

  • Kim, Hong-Joe;Oh, Ji-Yeon;Kim, Dong-Kwan;Yun, Hong-Tai;Jung, Woo-Suk;Hong, Jeum-Kyu;Kim, Ki-Deok
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.176-182
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    • 2010
  • Occurrence of plant diseases is dependent on various factors in the agricultural system. Due to recent extensive environmental climate changes, i.e. global warming, agricultural systems such as planting dates and cultivars are being affected. Gradual transition in disease occurrence and incidence in the agricultural fields can also be affected by direct and/or indirect environmental changes. In this study, we evaluated disease occurrence and incidence in soybean plants to investigate whether it could be related with cultivars, planting dates and geographical differences in Korea in 2008. Soybean cultivars including 'Taekwang', 'Pungsan', 'Cheongja 3', 'Saeol', and 'Dawon' were planted in four different dates, May 15, June 1, June 15, and June 30, in two locations, Suwon, and Naju. Soybean diseases such as wild fire and bacterial pustule were mainly found depending on cultivars, planting dates, and areas. Wild fire occurred severely on cv. 'Taekwang' while bacterial blight did on cv. 'Dawon' among tested cultivars. Disease developments of wild fire and bacterial blight generally decreased in delayed planting regardless of cultivars.

Nodulation and Early Growth of Supernodulating Mutants in Soybean (초다 근류착생 돌연변이체 콩의 뿌리혹 형성 및 초기생육)

  • 이석하;이홍석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.16-21
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    • 1992
  • Increase in nodulation and nitrogen fixation was achieved partly through the isolation of supernodulating soybean mutant plants. This experiment was conducted to compare nodulation, nitrogen fixation, and early growth characters of wild type 'Bragg' with those of its supernodulating soybean mutant, 'nts 382' and 'nts 246'. At 31 days after planting, nodule dry weight of nts mutants was 2.5 to 3.7 times greater than that of Bragg. Higher nodulation of nts mutants showed the reduced top growth, indicating that photosynthates might be translocated and used for nodule growth attached to the root system. Total acetylene reduction activity was higher in nts mutants than Bragg, whereas specific acetylene reduction activity of nts mutant was the half of that of Bragg. Mixture of nts mutants and Bragg did not affect nodulation characters each other, suggesting that factors affecting supernodulating characters exist inside rather than outside the root system.

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Growth, Nitrogen Metabolism, and Nodulation of Hypernodulating Soybean Mutant Affected by Soil Fertility

  • Ha, Bo-Keun;Lee, Suk-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.2
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    • pp.145-149
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    • 2001
  • This study was performed to evaluate the growth and nodulation characters of hypernodulating soy-bean mutant, SS2-2, and to know the growth and yield performance of the mutant in infertile soil. Soil fertility was adjusted by mixing the different ratios of soil components including clay, river sand, and horticultural bed, which resulted in fertile and infertile soil. Dry weight, nitrogen concentration, and leaf nitrate reductase of each plant were measured around V6 stage (47 days after planting) and around R3 stage (82 days after planting). There were significant effects of soil fertility and soybean genotype on the total dry weights including root, nodule, stem, leaf, and pod dry weight at V6 and R3 stages. Total dry weight of hypernodulating mutant, SS2-2, was clearly less than that of its wild type, Sinpaldalkong 2. However, nodule development on the roots of SS2-2 was much greater than that of Sinpaldalkong 2, regardless of soil fertility. Though SS2-2 was smaller in plant size than Sinpaldalkong 2, genotypic difference in total nitrogen content was not significant at both V6 and R3 stages because SS2-2 fixed more nitrogen biologically than its wild type in the root nodule. The SS2-2 mutant showed lower plant yield in both infertile and fertile soil. The SS2-2 contained more crude seed protein than Sinpaldalkong 2, and was characterized with reduced top and root growth.

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