Variation of Seed Component Contents in Wild Soybean(Glycine soja Sieb. & Zucc.)

야생콩(Glycine soja Sieb. & Zucc.)의 종실성분 함량변이

  • Published : 2005.11.01

Abstract

Studies were conducted to develop new breeding resources using wild soybean (Glycine soja Sieb. & Zucc.) species. In 132 wild soybeans, the average crude protein content was $45.4\%$, ranged from $37.4\%\;to\;50.2\%$ and average crude oil content was $9.3\%$ showing lower than cultivated soybean. The average total amino-acids content was $19,214\;{\mu}g/g$, containing $252\;{\mu}g/g$ of methionine and $103\;{\mu}g/g$ of cysteine, a sulfur containing amino-acid. In ratio of individual amino-acids, the glutamic acid, methionine and cystein content was $16.1\%,\;1.3\%,\;and\;0.5\%$, respectively. The correlation between total amino-acid and sulfur containing amino-acid was not significant. Total isoflavone concentration was, on average, $1,105\;{\mu}g/g$ in wild soybean germplasm. As a result of comparison with total isoflavone and genestein contents, significant positive correlations were observed between total isoflavone and genistein concentration.

본 실험은 콩의 단백질함량을 높이고 단백질 특성을 개선시키며, 고 기능성 보유 콩을 육성하기 위하여 수행되었다. 야생콩의 조단백, 조지방, 아미노산조성 및 isoflavone 함량을 분석 하였으며 결과를 요약하면 아래와 같다. 1. 야생콩 132점의 조단백질함량은 평균 $45.4\%$이며, 함량범위는 $37.4\~50.2\%$이었으며, 조지방함량은 평균 $9.3\%$으로 일반 재배종콩에 비하여 낮은 경향을 나타냈다. 2. 총 아미노산의 평균 함량은 $19,214{\mu}g/g$, 함량범위는 $16,098\~22,112{\mu}g/g$ 수준이었으며, 황함유 아미노산인 methionine은 평균 $252.7{\mu}g/g$, cysteine은 $102.9{\mu}g/g$의 함량을 나타냈다. 3. 전체 아미노산 함량 중 methionine과 cysteine은 각각 $1.3\%,\;0.5\%$의 비중을 차지하였으며, glutamic acid가 $16.1\%$로 가장 높았다. 4. 전체 아미노산 함량과 황함유 아미노산과의 상호관련성은 발견되지 않았으며, 충남 부여수집종인 IT182823 과 경기 포천 수집종인 IT184213이 높은 황함유 아미노산 함량을 나타냈다. 5. 총 아이소플라본의 평균 함량은 $1,105{\mu}g/g$이었으며, aglycone 중 genistein의 평균 함량이 $695.2{\mu}g/g$으로 가장 높았으며, 다음으로 daidgein, glycitein 순이었다. 6. Isoflavone과 genistein과의 상호관련성에 있어서 시험재료는 isoflavone 함량이 높으면 genistein 함량이 높은 경향이었다.

Keywords

References

  1. Hajika, M. Takahashi, S. Sakai and R Matsunaga. 1998. DominantInheritance of a Trait Lacking ~-conglycinin detected in a wildsoybean line. Breeding Science 48 : 383-386.
  2. Hoeck J, A, Walter R Fehr, Patricia A. Murphy and Grace A. Welke.2000. Influence of Genotype and Environment on Isoflavone Contentsof Soybean. Crop Science 40: 48-51
  3. Kennedy, A. 1995. The evidence for soybean products as cancer preventiveagents. 1.Nutr. 125 : 733-743.
  4. Kenneth K. C and E. M. Kurowsaka. 1995. Soy consumption and cholesterolreduction: Review of animal and human studies. 1. Nutr.125 : 594-597.
  5. Kim, S. R and S. D. Kim. (1996) Studies on soybean isoflavonesRDA. J. Agri. Sci. 38: 155-165.
  6. Kim, Y. H., S. D. Kim and E. H. Hong. 1995. Present status and perspectivesof soybean breeding program for high quality in Korea.Korea Soybean Digest 12(2) : 1-20.
  7. Kitamura, K and N. Kaizuma. 1981. Mutation strains with low level ofsubunit of7S globulin in soybean(Glycine max Merr.) seed. Japan1. Breed. 31(4): 354-359.
  8. Kudou, S., M. Shimoyamada., T. Imura. T. Uchida and K Okubo.1991. A new isoflavone glycoside in soybean seeds(Glycine maxMerrill), Glycitein 7-0-~-D-(6"-O-Acetyl)-Glucopyranoside,Agric. BioI. Chern. 55(3) : 859-860.
  9. Messina, M. 1998. Soyfoods, Soybean Isoflavones, and Bone Health.Korea Soybean Digest 15(2): 122-136.
  10. Nairn, M. B., B. Gestetner, S. Zikah, Y.Birk, and A. Bondi. 1974. Soybeanisoflavones, characterization, determination and antifungalactivity. J, Agr. Food Chern. 22: 806-810.
  11. Ogawa, T., E. Tayama, K Kitamura, and N. Kaizuma. 1989. GeneticImprovement of seed storage using three variant alleles of 7s globulinsubunits in soybean (Glycine max L.) Japan 1.Breed. 39 : 137147.
  12. Sebolt, A.M. RC. Shoemaker, and RW. Diers. 2000. Analysis of aQuantitative Trait Locus Allele from Wild Soybean That IncreasesSeed Protein Concentration in Soybean. Crop Sci. 40(5) : 14381444.
  13. Takahashi, K, Y. Mizuno, S. Yumoto, K Kitamura and S. Nakamura.1996. Inheritance of the a-subunit deficiency of [l-congjycinin insoybean (glycine max L. Merrill)line induced by y-ray irradiation.Breeding Science 46 : 251-255
  14. ~:t,t~'t5-j::fJ ilip. 2000. )7"1 AT i- l'v"f''! Gly m Bd 28KO);i{~~Atil{tl(il~H':$tt ~~JL mj:~fn. 2(302): 161.
  15. Walter, E. D. 1941. Genistein(an isoflavone glucoside) and its aglicon,genistein, from soya beans. J. am. Soc. 63 : 3273-3275.
  16. Wilcox, 1. R. 1998. Increasing seed protein in soybean with eightcycles ofrecurrent selection. Crop Sci., 38 : 1536-1540,
  17. Yi,M.A., T.W.Kwon. and J.S. Kim. (1997) Changes in isoflavone contentsduring maturation ofsoybean seed. 1.Food Sci. 2(3) : 255-258.