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http://dx.doi.org/10.5423/PPJ.OA.02.2014.0014

Molecular and Biological Characterization of an Isolate of Cucumber mosaic virus from Glycine soja by Generating its Infectious Full-genome cDNA Clones  

Phan, Mi Sa Vo (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University)
Seo, Jang-Kyun (Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration)
Choi, Hong-Soo (Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration)
Lee, Su-Heon (Department of Applied Biology, Kyungpook National University)
Kim, Kook-Hyung (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University)
Publication Information
The Plant Pathology Journal / v.30, no.2, 2014 , pp. 159-167 More about this Journal
Abstract
Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV) from Glycine soja (wild soybean), named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean) and Pisum sativum (pea) as well as N. benthamiana, but not the other legume species.
Keywords
Cucumber mosaic virus; sequence analysis; phylogenetic analyses; infectious clones; wild soybean;
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