• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.04a
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• pp.18-21
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• 2002

Phytoremediation which uses plants to enhance the bioremediation through stimulation of microbial activity and root uptake, has been a topic of increasing interest. Mathematical model were developed that can be applied to various bioremediation methods in the unsaturated zone, especially phytoremediation, for simulating the fate and transport of contaminants under field conditions. A 2-year field study was conducted using 72 (1.5m long and 0.1 m diameter) column lysimeters with four treatments: Johnsongrass; wild rye grass; a rotation of Johnsongrass and wild rye grass; and unplanted fallow conditions. The developed model represented the fate and transport of contaminant both in vegetated and unplanted soils satisfactorily for field applications. Parameters related to the contaminant concentration in the water phase were the main parameters determining the contaminant fate in the vadose zone and indicated that the bioavailability can be the most important factor in the success of phytoremediation as well as bioremediation applications.

• Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.107-115
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• 2012

In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chromatography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two parasites, the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of $57.9^{\circ}C$ and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid isopod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem.

• Proceedings of the Korean Society of Soil Zoology Conference
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• 1985.01a
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• pp.8-8
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• 1985

• Journal of fish pathology
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• v.23 no.1
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• pp.107-112
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• 2010

Parasitism of Pseudocaligus sp. in wild grass puffer Takifugu niphobles in the coast of Yeosu in spring, 2007 was identified. The preadult and adult stages of Pseudocaligus sp. were observed, which consisted of cephalothorax, genital segment and abdomen. Lunules were present on the anterior margin of the frontal plate. In order to develop the treatment method, we examined the effects of formalin and fresh water on the parasite. The Pseudocaligus sp. were detached from the host fish by bathing at concentrations of 50 to 400 ppm formalin within 60 min, but 57% was exterminated to fresh water for 90 min.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.1
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• pp.57-65
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• 2005

This study was performed to investigate the effects of very low calorie diet (VLCD) using meal replacements that contain the wild grass extracts that are consisted of mainly Samul-tang ingredients on weight reduction and health promotion in the obese women (BMI$\geq$25 kg/$m^2$) for four weeks. Fifty six premenopausal women participated in this experiment. Each subject was either classified as part of the control group (C group, n=28) that consumed 3 regular meals a day or the meal replacement group (MR group, n=28) that consumed 1 regular meal and 2 meal replacements within 600 kcal/day. Anthropometric measurements, body composition, health related factors, and psychological factors were assessed before (the initial) and after (the 4$^{th}$ week) the study. Obesity was significantly decreased after the diet intervention. Anthropometric measurements such as weight, waist and hip circumference, and BMI and body composition such as body fat, and fat mass were significantly decreased in all groups after the diet intervention. Anthropometric measurements and body composition of the MR group were significantly decreased more than those of the C group. Serum total cholesterol was significantly decreased in the MR groups but not in the C group and serum triglyceride concentration was significantly decreased in all groups after the diet intervention. All the biochemical measurements were within normal ranges after the diet intervention. Psychological factors such as stress and depression were significantly decreased in two groups after the diet intervention. Therefore, VLCD using meal replacements is very effective for weight reduction in the obese premenopausal women.

• The Korean Journal of Soil Zoology
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• v.6 no.1_2
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• pp.11-16
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• 2001

This study was carried out to investigate the species composition and its abundance in the heap of compost, wild-grass, and sewer. 1,525 individuals were collected from 11th July, 2000 to 3rd August, 2000 at the 108 sites. They encompassed 3 families, 4 genera and 6-8 species. Species composition and its percentile abundance were as follows: Eisenia fetida 86.2%, Aporectodea trapezoides 0.5%, Amynthas hilgendorfi 1.1%, Amyntha agrestis 2.8%, A. koreanus group 6.3%, Drawida sp. 17%, Others 1.4fo in the compost heap, Eisenia fetida 9.8%, Aporectodea trapezoides 9.1%, Amynthas hilgendorfi 12.1%, Amyntha agrestis 25.1%, A. koreanus group 27.8%, Drawida sp 2.8%, Others 13.2% in the wild-grass and Eisenia fetida 24.2%, Aporectodea trapezoides 5.4%, Amynthas hilgendorfi 10.2%, Amyntha agrestis 5.4%, A. koreanus group 26.3%, Drawida sp 1.6%, Others 22.6% in the sewer.

• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.105-116
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• 2011

Transgenic zoysiagrass (Zoysia japonica Steud.) expressing the bar gene inserted in the plant genome has been generated previously through Agrobacterium tumefaciens-mediated transformation. The GM zoysiagrass (event: JG21) permits efficient management of weed control of widely cultivated zoysiagrass fields, reducing the frequency and cost of using various herbicides for weed control. Now we have carried out the environmental risk assessment of JG21 prior to applying to the governmental regulatory agency for the commercial release of the GM turf grass outside of test plots. The morphological phenotypes, molecular analysis, weediness and gene flow from each test plot of JG21 and wild-type zoysiagrasses have been evaluated by selectively analyzing environmental effects. There were no marked differences in morphological phenotypes between JG21 and wild-type grasses. The JG21 retained its stable integration in the host plant in T1 generation, exhibiting a 3:1 segregation ratio according to the Mendelian genetics. We confirmed the copy number (1) of JG21 by using Southern blot analysis, as the transgenic plants were tolerant to ammonium glufosinate throughout the culture period. From cross-fertilization and gene flow studies, we found a 9% cross-pollination rate at the center of JG21 field and 0% at distances over 3 m from the field. The JG21 and wild-type zoysiagrass plants are not considered "weed" because zoysiagrasses generally are not dominant and do not spread into weedy areas easily. We assessed the horizontal gene transfer (HGT) of the transgene DNA to soil microorganisms from JG21 and wild-type plants. The bar gene was not detected from the total genomic DNA extracted from each rhizosphere soil of GM and non-GM Zoysia grass fields. Through the monitoring of JG21 transgene's unintentional release into the environment, we found no evidence for either pollen mediated gene flow of zoysiagrass or seed dispersal from the test field within a 3 km radius of the natural habitat.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.2
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• pp.78-87
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• 1999

This study was aimed to establish the method and process of the Biotope Mapping System.(B.M.S.) The main aspects of research for biotope were divided by 4 schemes such as biosphere, geosphere, antroposphere and evaluation informations. The cartographic function of GIS(geographic information system) was also used to mapping them. The B.M.S. had been progressed with 5 steps : (1) making research goals, (2) constructing the graphic and attribute databases, (3) classifying 3 types of biotope such as tree/shrub biotop, grass biotop and wetland biotop. (4) cross-analyzing biotope types against land use, habitat characters, relief characters and danger/disturbance elements, (5) evaluating the biotope's potentialities. Only 3 steps of them were done in this study. The results of applicating the B.M.S on the research site as followings : 1. The interdisciplinary researches were needed to develop the B.M.S in Korea since ecological habitat conditions of Germany, England are differed from them of Korea. 2. The distributions of the land uses in Maejuri, Seunghwan eup in Chonan city were mainly recorded by forest(29.82%), orchard(14.11%) and landscaping around building site(9.05%). 3. The percentages of the Tree/Shrub Biotope type were ranged by the orchard type(23.8%), natural and artificial deciduous type with natural coniferous(23.6%) and etc. 4. The Grass Biotope type was mainly recorded by the wild grassland type(27.99%), garden type(14.21%) and peddy field with wild grassland(13.24%). 5. The distributions of the wetland were pointed high at the wetland type with reed marsh and edge vegetation around wetland(54.79%) and etc.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.648-655
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• 2010

This study is the first comprehensive report on the molecular cloning, structural characterization, sequence comparison between wild and mutant types, copy number in the genome, expression features and activities of a gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in Korean lawn grass ($Zoysia$ $japonica$). The full length cDNA of the EPSPS from Korean lawn grass ($zj$EPSPS) obtained from a 3' and 5' RACE method was 1540 bp, containing a 1176 bp ORF, a 144 bp leader sequence (5' UTR) and a 220 bp 3' UTR, which was eventually decoded 391 amino acid residues with a molecular mass of 41.74 kDa. The Southern blot detection of the $zj$EPSPS showed that the gene exists as a single copy in the Korean lawn grass genome. Sequence comparison of the $zj$EPSPS gene demonstrated that the glyphosate-tolerant mutant (GT) having a Pro-53 to Ser substitution in the gene seems to have a preferred binding activity of the enzyme to phosphoenol pyruvate(PEP) over glyphosate, which allows the continuous synthesis of aromatic amino acids in the shikimate pathway. From the Northern blotting analysis, the $zj$EPSPS was found to be highly expressed, with continuous increase until 36 hours after 0.5% glyphosate treatment in both wild and mutant samples, but 1.5-fold higher EPSP synthase activity was observed in the tolerant mutant when exposed to the glyphosate treatment. The molecular information of the $zj$EPSPS gene obtained from this study needs to be further dissected to be more effectively applied to the development of gene-specific DNA markers and zoysiagrass cultivars; nevertheless, the glyphosate-tolerant mutant having the featured $zj$EPSPS gene can be provided to turfgrass managers for weed problems with timely adoptable management options.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.4
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• pp.392-406
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• 1985

The soil management practices before transplanting the ginseng plant were studied with two organic matter sources such as a traditional organic matter (wild grass) and commercial organic fertilizer (byproducts of amino acid fermantation) during the late spring to late autumn. During the soil management practices, the soil received 40kg N/10a from five different combination treatments with above two organic matter sources, a wild grass and a commercial organic fertilizer. After the application of the treatments, the soil were ploughed regularly at the interval of 20 days and the changes of physicochemical properties during the soil management practices were investigated. The next year after soil management practices, ginseng plants were transplanted to each treatment, growth and the content of some organic components of ginseng plant were measured for comparision of the different treatments. 1. The decrease in bulk density observed during the first 40 days of management was considered to be the effect of the improved physical conditions caused by ploughing, The decrease in bulk density observed after 40 days of management was considered to be the effect of organic matter. Similar results were observed in particle density, however porosity increased with time. 2. Soil pH tended to decrease during the first 40 days of management, after which period the pH increased and was stabilized. However, CEC increased with organic matter treatment and the exchangeable $NH^+_4-N$ and $NO^-_3-N$ increased in 20 and 40 days after the management practices, respectively, and after that period it became steady. 3. The decomposition rate of treated organic matter was measured by the incubation test in laboratory conditions. The rate of decomposition was rapid during the first 20 days of management, after which period it showed slight changes. 4. The weight of ginseng root significantly increased in the treatment of 10kg N/10a organic fertilizer and 30kg N/10a wild grass. 5. The saponin content of ginseng root was highest in the 40kg N/10a wild grass treatment. The addition of organic fertilizer at the rate of more than 20kg N/10a caused the decrease in the saponin content.