• 생약학회지
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• 제48권3호
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• pp.255-259
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• 2017

This study was conducted to provide basic information on ginseng saponin of dried ginseng radices. In order to achieve the proposed objective ginsenoside compositions of dried ginseng radices extract with 70% ethyl alcohol were examined by HPLC. The total saponin content, the sum of all ginsenosides, showed that Wild simulated ginseng (WSG), White fine ginseng (WFG), Skin White ginseng (SWG), and White ginseng (WG) stood at 2.510%, 1.643%, 0.587, and 0.429%, respectively. WSG in PPD/PPT ratio was highest at 3.190, WFG (1.934), WG (1.600), SWG (1.386) in order. In the content of ginsenoside Rb1, one of the marker compounds of ginseng, WSG (1.095%) showed the highest content, and WFG (0.527%), SWG (0.246%), WG (0.133%) in this order. The content of ginsenoside Rb1 of WSG (1.095%) was 4.5 times higher than SWG (0.246%). WSG (0.230%) showed the highest content in ginsenoside Rg1, a marker compounds of ginseng, followed by WFG (0.180%), SWG (0.141%) and WG (0.086%). The content of ginsenoside Rg1 of WSG (0.230%) was 1.6 times higher than SWG (0.141%).

• 대한약침학회지
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• 제6권2호
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• pp.7-27
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• 2003

Objective : The purpose of this study was to investigate acute and subacute toxicity and sarcoma-180 anti-cancer effects of herbal acupuncture with cultivated wild ginseng (distilled) in mice and rats. Method : Balb/c mice were injected intravenous with cultivated wild ginseng herbal acupuncture for $LD_{50}$ and acute toxicity test. Sprague-Dawley rats were injected intravenous with cultivated wild ginseng herbal acupuncture for subacute toxicity test. The cultivated wild ginseng herbal-acupuncture was injected at the tail vein of mice. Results : 1. In acute $LD_{50}$ toxicity test, there was no mortality thus unable to attain the value. 2. Examining the toxic response in the acute toxicity test, there was no sign of toxication. 3. In acute toxic test, running biochemical serum test couldn't yield any differences between the control and experiment groups. 4. In subacute toxicity test, there was no sign of toxication in the experimental groups and didn't show any changes in weight compared to the normal group. 5. In subacute toxicity test, biochemical serum test showed significant increase of Total albumin, Albumin, and Glucose in the experimental group I compared with the control group. Significant decrease of GOT, ALP, GPT, and Triglyceride were shown. In experiment group II, only Glucose showed significant increase compared with the control group. 6. Measuring survival rate for anti-cancer effects of Sarcoma-180 cancer cell line, all the experimental groups showed significant increase in survival rate. 7. Measuring NK cell activity rate, no significant difference was shown throughout the groups. 8. Measuring Interleukin-2 productivity rate, all the experimental groups didn't show significant difference. 9. For manifestation of cytokine mRNA, significant decrease of interleukin-10 was witnessed in the experimental group compared to the control group. Conclusion : According to the results, we can conclude cultivated wild ginseng herbal acupuncture caused negligible toxicity, and had anti-tumor effects in mice.

• 한방비만학회지
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• 제13권2호
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• pp.84-87
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• 2013

2명의 가성여성형유방 환자에게 산삼약침을 주 1회, 총 5회 시술을 하고 시술 전후 사진 촬영을 통해 약침의 효과를 확인한 결과, 한 증례에서는 시술 전후 명백한 형태상의 변화가 관찰되었으나 또 다른 증례에서는 변화가 미미하였다. 향후 보다 더 많은 환자를 대상으로 산삼약침의 치료효과에 대한 검증이 필요하며, 효과에 미치는 간섭요인이 확인되어야 할 것이다. 더불어 여성형유방의 경과에 대한 객관적인 평가방법의 개발 역시 필요할 것으로 생각된다.

• Toxicological Research
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• 제21권1호
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• pp.51-55
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• 2005

To investigate the acute toxicity of adventitious roots extract derived from wild ginseng, it was orally administered to beagle dogs with a single dose. In acute toxicity test, three groups (9 beagle dogs of male) were administered with different dosages of adventitious roots extract (prepared by Biopia Corp.) 500 mg/kg (G2), 1,000 mg/kg (G3), 2,000 mg/kg (G4) and one group (G1, 2 beagle dogs of male) were received by only capsule without the extract according to the Regulation on Korea Food and Drug Administration (1999. 12. 22). There were vomitus for a time and mucous stool at the day, and anorexia and mucous stool at the first day in the group of 2,000 mg/kg administration. There were mucous stool in one and anorexia for a while in two beagle dogs at the first day in the 1,000 mg/kg administration. But no death or abnormal clinical sign was observed through the study period. Therefore, the adventitious roots extract derived from wild ginseng is considered not to have the acute toxicity in the beagle dogs. These results suggest that LD/sub 50/ value of the test substance was considered to be more than 2,000 mg/kg in the beagle dogs.

• Toxicological Research
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• 제21권1호
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• pp.57-62
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• 2005

To investigate the mutant induction of wild ginseng culture extract, we performed chromosomal aberration assay with chinese hamster lung cell in vitro. The test concentration of the extract was decided for the standard with the 50% suppression of cell propagation in the cell. The concentrations for the chromosome test were 1,250, 2,500 and 5,000 ㎍/ml with metabolic activation (+S, 6 hours treatment), 1,100, 2,200 and 4,400 ㎍/ml without metabolic activation (-S, 6 hours treatment) 800, 1,600 and 3,200 ㎍/ml without metabolic activation (-S, 24 hours treatment). No significant increase in chromosome aberrations was observed at any of these concentrations both in the absence and presence of metabolic activation system. Cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS) caused a significant increase in chromosome aberration. These results may be concluded that wild ginseng culture extract is not capable of inducing chromosome aberration in cultured chinese hamster lung cell regardless of metabolic activation and genotoxicity of that is negative under the present experimental condition.

• 한국자원식물학회지
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• 제34권5호
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• pp.403-410
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• 2021

본 연구는 산양삼 7년근과 13년근을 대상으로 적변 유무에 따라 분류하고 적변에 따른 이들의 생육특성과 진세노사이드 함량을 조사하기 위해 수행되었다. 산양삼 재배지 9개소의 산양삼 지하부의 생육특성을 분석한 결과, 7년근과 13년근의 생육특성은 적변에 따른 유의적인 차이는 확인되지 않았다. 적변에 따른 연근별 산양삼의 진세노사이드 함량을 비교 분석한 결과, 7년근은 Rb1과 Rg1의 함량이 일반 산양삼에 비해 적변 산양삼에서 높았고, Rc, Rd, Re, Rg2는 적변 산양삼에 비해 일반 산양삼에서 유의적으로 높은 것을 확인하였으나 13년근의 진세노사이드 함량은 유의적인 차이를 확인할 수 없었다. 산양삼 생육특성과 진세노사이드 함량 간의 상관관계를 분석한 결과, 일반 산양삼의 경우 진세노사이드 Rb2, Rc, Rd, Rf, Rg1이 산양삼의 지하부 길이와 유의적인 정의 상관관계를 보였고, 적변 산양삼은 진세노사이드 Rd가 지하부 길이와는 유의적인 정의 상관관계, 주근직 경과는 유의적인 부의 상관관계를 보였다. 본 연구에서는 7년근과 13년근 모두 적변 여부에 따른 생육특성의 차이는 확인되지 않았고, 7년근의 진세노사이드 함량은 적변에 따라 유의적인 차이를 보였지만 13년근은 유의적인 차이가 없는 것을 확인하였다. 임가에서 산양삼은 7년근에 비해 13년근이 보다 고가에 거래되는 만큼 본 연구의 결과를 통해 적변 산양삼에 대한 소비자들의 인식을 개선하여 산양삼 재배농가의 소득증대에 도움을 주고, 향후 산양삼의 품질규격을 정립하고 유통체계를 수립함에 유용한 정보를 제공할 수 있을 것으로 사료된다.

• Korean Journal of Acupuncture
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• 제24권4호
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• pp.181-200
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• 2007

Objevtives & Methods : Effects of cultivated wild ginseng pharmacopuncture at BL18 and LI11 on lipid composition, cytokine level, liver function, anti-oxidative capacity and histological characters were investigated in diet-induced obese rats. Forty male Sprague-Dawley rats weighing about 400g were divided into 4 groups of control, BL18, LI11 and BL18 plus LI11 pharmacopuncture groups and raised for 4 weeks. Results : 1. Plasma ${\beta}$-lipoprotein, free fatty acids level and TNF-${\alpha}$ levels significantly decreased in the pharmacopuncture groups compared to those of no treatment group. Plasma and liver total cholesterol, triglyceride, glucose and thiobarbituric acid reactive substance (TBARS) levels were also significantly lower than those of no treatment group. There was, however, no difference in TBARS level among pharmacopuncture groups. Liver total cholesterol level of BL18 pharmacopuncture group was lower than those of the other two pharmacopuncture groups. In LDL-cholesterol level, BL18 pharmacopuncture and BL18 plus LI11 pharmacopuncture groups only had significantly lower levels than that of no treatment group. 2. There was no significant difference between cultivated wild ginseng pharmacopuncture groups and no treatment group in IL-6, alanine transaminase (ALT), aspartic acid transaminase (AST) levels. 3. Compared with \ those of no treatment group, pharmacopuncture groups had significantly higher levels of HDL-cholesterol, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase activities. There was, however, no significant difference among pharmacopuncture groups. 4. Histological characters of heart, kidney and liver of BL18 pharmacopuncture group were similar to those of normal rats. Conclusions : These results indicate that cultivated wild ginseng pharmacopuncture at BL18 and LI11 may suppress adipose tissue mass and lipid peroxidation and activate antioxidant system.

• 동의생리병리학회지
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• 제27권2호
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• pp.233-238
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• 2013

The development of healthy beverages are increasing by big concerns for well-being. The aim of this study is to develop cultivated wild ginseng-Kyungohkgo (CKOG) beverage using cultivated wild ginseng instead of natural wild ginseng for Kyungohkgo. To examine physiochemical properties of CKOG beverage, pH, degree Brix, acidity, Huntor color, particle size and sedimentation volume were measured. When compared with commercial KOG beverage, CKOG beverage was lower in pH, acidity, particle size and higher in sedimentation volume. In the sensory evaluation of CKOG beverage, the best recipe of healthy CKOG beverage was 20% CKOG, 5.7% honey, 0.2% citric acid and 74.1% of water. In stability testing, pH, degree Brix, acidity, Huntor color of CKOG beverage was not changed, microbes in CKOG beverage was not detected for 60 days. These results suggest that CKOG may have a possibility of development as healthy beverage.

• Journal of Acupuncture Research
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• 제32권2호
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• pp.97-103
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• 2015

Objectives : This study was aimed to report the anti-wrinkle effects of cultivated wild ginseng pharmacopuncture. Methods : Data were collected from 23 patients who had been treated with cultivated wild ginseng pharmacopuncture for skin wrinkles. Patients were treated twice a week, for a total of 5 times. To observe the effect of the treatment, we measured the width and depth of skin wrinkles with ECOSKIN(portable fluorescent dermal diagnostic equipment). We also conducted a survey with patients to measure total satisfaction and the anti-wrinkle effect using a semantic differential scale. The evaluation was done 2 times, once before the first treatment and once 1 week after the last treatment. The relationship was measured by Wilcoxon signed rank test. Results : Skin wrinkle mean width decreased significantly from $9.45{\pm}6.60$ to $5.54{\pm}4.78$. Depth decreased significantly from $0.68{\pm}0.34$ to $0.39{\pm}0.24$ total satisfaction increased significantly from $2.46{\pm}0.743$ to $4.13{\pm}0.51$. Anti-wrinkle effect increased significantly from $2.46{\pm}0.63$ to $3.93{\pm}0.593$. Conclusions : These results suggest that cultivated wild ginseng pharmacopuncture may be effective for decreasing wrinkles.

• 산업식품공학
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• 제21권3호
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• pp.201-207
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• 2017

It is well-known that cultivated wild Panax ginseng has anti-inflammatory effect. However, a comparative study on cultivation period vs biofunctionality is currently lacking. In this study, 70% ethanol extracts of 3-years (yrs)-, 5-yrs-, or 7-yrs-old cultivated wild ginseng were evaluated for their inhibitory effects on RAW264.7 murine macrophages. Specifically, the production of pro-inflammatory cytokines (interleukin-6 [IL-6] and tumor necrosis factor-alpha [TNF-${\alpha}$]), the expression of surface proteins (CD80, CD86, and MHC-II), and the phagocytic properties were investigated. RAW264.7 cells were induced by 500 ng/mL of lipopolysaccharide (LPS) and treated with 0.1, 1, and 10 ppm of samples. LPS-induced IL-6, TNF-${\alpha}$ and surface proteins in all samples were down-regulated in a dose-dependent manner. Both IL-6 and TNF-${\alpha}$ were significantly reduced at 10 ppm of the 7-yrs-old sample compared to 10 ppm of 3-yrs- and 5-yrs-old samples. CD80 and CD86 were also reduced at 10 ppm of all samples, and there was no difference among samples. The phagocytosis has no difference except in 10 ppm of 3 yr-old sample. The results suggest that cultivated wild ginseng extract has anti-inflammatory effect without decreasing phagocytosis.