• Molecules and Cells
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• 제28권1호
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• pp.57-65
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• 2009

CDC48 is a member of the AAA ATPase superfamily. Yeast CDC48 and its mammalian homolog p97 are implicated in diverse cellular processes, including mitosis, membrane fusion, and ubiquitin-dependent protein degradation. However, the cellular functions of plant CDC48 proteins are largely unknown. In the present study, we performed virus-induced gene silencing (VIGS) screening and found that silencing of a gene encoding a tobacco CDC48 homolog, NgCDC48, resulted in severe abnormalities in leaf and shoot development in tobacco. Furthermore, transgenic tobacco plants (35S:anti-NgCDC48), in which the NgCDC48 gene was suppressed using the antisense RNA method, exhibited severely aberrant development of both vegetative and reproductive organs, resulting in arrested shoot and leaf growth and sterile flowers. Approximately 57-83% of 35S:anti-NgCDC48 plants failed to develop mature organs and died at early stage of development. Scanning electron microscopy showed that both adaxial and abaxial epidermal pavement cells in antisense transgenic leaves were significantly smaller and more numerous than those in wild type leaves. These results indicate that NgCDC48 is critically involved in cell growth and development of tobacco plants. An in vivo targeting experiment revealed that NgCDC48 resides in the endoplasmic reticulum (ER) in tobacco protoplasts. We consider the tantalizing possibility that CDC48-mediated degradation of an as-yet unidentified protein(s) in the ER might be a critical step for cell growth and expansion in tobacco leaves.

• 한국환경복원기술학회지
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• 제21권3호
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• pp.47-56
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• 2018

Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

• 한국자원식물학회지
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• 제4권2호
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• pp.67-74
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• 1991

동양에서는 생약을 주로 한약조제에 많이 이용되고 있으나 서양에서는 신의약품 개발과 제조에 이용되고 있다. 그러나 최근에 와서는 국내외를 막론하고 제약업계에서 양약의 제조와 신의약품 개발에 치중하여 생약의 수요가 증가하고 있다. 따라서 당국에서는 계획생산에 대한 지도와 자금지원, 품종관리, 재배 및 신의약품개발에 대한 연구지원, 수입통제에 의한 농민보호등 제도적 지원을 강화하여 우리나라 주종 재배약초의 자포자기적인 생산기피현상을 막아야 할 것이다.

• 한국조경학회지
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• 제23권1호
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• pp.111-122
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• 1995

12 populatiDns of Ch indicum L. and it's variation antogenous in Tforea were selected throughout the country and their morphological characteristics were studied in this paper. The results are as follows. 1) The mean of the plant height is 97.3cm. Ch. indicunl is 76.5cm and Ch. boreale is 116. 6cm in height. The number of nodes is 58 on the average. The color of the stem is generally black-red in the case of Ch. indicum and is green for Ch. boreale. 2) The leaves of Ch. inficum are smaller In size and thicker than chose of Ch. boreale, and it has a stipule. This fact enables us to distinguish one species from the other, however, the Gyukpo-population of Ch. indicum has a large leaf, and Byunsan-population of Ch. boreale has a stipule so that the above fact is not an exact criterion on which to base one classification. 3) The size of the capitulum is 24.9mm for Ch. indicum and 15.6mm in the case of Ch. boreaje so that the difference between species is admitted. It was confirmed hat the size of the capitulum has positive correlation to that of the pedicel and has egative correlation to the number of flowers, so that of inflorescence, Ch. indicumhas corymb, and Ch. boreale has crowed corymb or umbel-type.The number of ligulates is 19 on the average, which no the difference being founnd between species and populations.4) The cluster analysis of morphological characteristics showed that Ch. indicum wasdivided into two groups, which was shown thought to be due to the difference of speciesbetween Ch. indicum and Ch. boreale. However, it could not be shown that their morphological characteristics explain a geographical relationship between populations

• 한국환경복원기술학회지
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• 제1권1호
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• pp.141-150
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• 1998

This study was conducted to find out the possibility of seeding of Zoysia japonica and woody plants for the revegetation of rock-exposed-cutslopes by Natural Topsoil Restoration Methods (NTRM). The 5cm thick layer of artificial soil was attached at rock-exposed cutslopes for the growth of Zoysia japonica and native woody plants by NTRM. The main results were summarized as follows. 1. Artificial soil attached at rock-exposed-slopes by NTRM was not eroded until recovered by Zoysia japonica and native woody plants. It shows extremely low soil erodibility. 2. One year later after seeding, woody plants show 100% ground coverage. But, in early stage, they grew more quickly at northwest slopes than at southeast slopes. 3. In seeding of Zoysia japonica, it grew more quickly at southeast slopes than at northwest slopes. Also, it grew better at lower part of the slopes than at middle part of the slopes. 4. After one year from seeding, the height of Lespedeza cyrtobotrya and Indigo/era pseudo-tinctoria were longer than any other plants. The next were Albizzia julibrissin, Lespedeza cuneata and wild flowers. The tree height of Rhus chinensis shows 28cm length, so it has enough possibility to be used for roadside revegetation works. 5. Lespedeza cyrtobotrya and Indigo/era pseudo-tinctoria show different importance values according to slope aspect. But, they show tendency to outweigh the other plants at all slope-aspects. 6. Most of the plants seeded by NTRM were germinated well and they covered rock-exposed-slopes so quickly and effectively. Thus it leads to conclusion that the revegetation methods used in this experiment was a very effective methods to recover rock-exposed-slopes by seeding of zoysia japonica and native woody plants.

• Natural Product Sciences
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• 제27권1호
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• pp.36-44
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• 2021

The essential oils from the aerial parts (leaves and flowers) of Spiraea hypericifolia L. (Rosaceae), collected in Northern Kazakhstan, were obtained by distillation in two dispersion media (distilled water and 15% NaCl solution). The chemical composition of the essential oils was evaluated by GC-MS for the first time. The yield of the essential oil was 0.04% (in fresh growth conditions) and 0.02% (in dry growth conditions) respectively regardless of which dispersion media (H2O or 15% NaCl solution) was used at the isolation of essential oil. The main compounds were aliphatic hydrocarbons (alkanes) (40.6-53.2%), aldehydes (8.4-17.4%), diterpenoids (9.1-16.7%) and ketones (6.2-8.7%). Content of monoterpenoids was depended on dispersion media (2.2-3.6% where H2O was dispersion media and 8.4-8.5% where 15% NaCl solution was dispersion media). n-Heneicosane (17.4-34.1%) and n-tricosane (14.3-19.5%) were the main constituents of the essential oil of S. hypericifolia. There were many insects from different classes in S. hypericifolia at flowering. Important components such as α-methylene-γ-butyrolactone (0.8-2.8%), benzyl cyanide (0.7-1.1%), β-damascenone (1.2-2.9%), (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (1.8-2.7%), β-ionone (0.5-1.8%) and others were detected in small amounts.

• 식물분류학회지
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• 제52권4호
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• pp.226-234
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• 2022

The study aims are to examine the characteristics of artificial and natural hybrids between Viola albida var. albida (= albida, from below) and V. albida var. chaerophylloides (= chaerophylloides, from below), and to confirm if hybrids could be fertile and make populations in their native habitats. The 1st filial (= F₁, from below) leaf shape produced by artificial crossing between albida and chaerophylloides was the same as that of V. albida var. takahashii (= takahashii, from below), and F₁ bore also both chasmogamous and cleistogamous flowers. F₁ seed number was 9.6 per cleistogamous pods, which was remarkably less than the average of 38.2 for albida and chaerophylloides, but the germination rate was all similar. The leaf type of self-crossed 2nd offsprings (= F₂, from below) showed all leaf types found in the Viola albida complex, but the ratio of chaerophylloides leaf type was relatively low. Individuals whose F₂ leaf type was restored to albida produced an average of 31.4 seeds per capsule, meaning that fertility was restored. On the other hand, individuals of F₂ takahashii leaf type come to fruition a low average of 10.4 seeds per capsule, which is similar to that of takahashii. The results of crossbreeding experiment, where is their native habitats, were similar to that of laboratory. Both albida and chaerophylloids in Mt. Bulmyeong distribute extensively, but takahashii make a small population only in places where albida and chaerophylloides grow together. Summarizing the above results is suggesting that the speciation of takahashii was done by hybrid between albida and chaerophylloides, and these have been maintained with relatively small population by cleistogamous capsules.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.51-51
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• 2022

Water dropwort (Oenanthe stolonifera DC.) is a perennial herbaceous plant that grows wild throughout Korea. As of 2020, 24,819 tons (51.7% of open field, 48.3% of facility) are produced in 1,278 ha (59.9% of open field, 40.1% of facility) nationwide. Water dropwort, which is rich in nutrients such as vitamins and iron, is mainly cultivated by vegetative propagation method using local traditional species, however, seed propagation and breeding of cultivars are insufficient so far. Since securing, propagating and continuous characterization of various genetic resources are required to breed new cultivars, this study was conducted to compare the main characteristics of domestic genetic resources and to improve their utilization. Growth characteristics such as plant height, fresh weight, plant type, and flowering date were investigated for the 89 varieties of genetic resources owned by Jeollabuk-do Agricultural Research and Extension Services after vegetative propagation in the individual pots. Also, the morphological image information of leaves and flowers was constructed. Genetic resources were collected nationwide and originated in 30 regions including Hwaseong, Siheung and Jeju. Their plant types could be classified into straight, intermediate and creeping types according to their morphological characteristics, and at this time, the number of intermediate types accounted for the largest proportion. Flowering was carried out under high-temperature and long-day conditions in summer. According to the flowering date, they could be classified into early, middle and late flowering varieties, and at this time, the middle flowering varieties occupied the largest proportion. As a result of the investigation of vegetative growth characteristics, varieties with long plant height, heavy fresh weight and thick stem thickness were identified. Along with this result, it is thought that classification and selection of genetic resources for various purposes will be possible through additional investigations such as analysis of components and antioxidant activity. Moreover, it is judged that such results can be used as basic data for breeding new water dropwort cultivars in the future.

• 한국초지조사료학회지
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• 제21권4호
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• pp.233-246
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• 2001

본 연구는 국산 야생화초지(Native wildflower p pasture: NWP, turf grass 6종 + 국산야생화 11종) 및 외국산 야생화초지(Introduced wildflower pasture; IWP, turf grass 6종 + 외국산야생화 9종)를 조성하여 계절별 개화시간, 개화지속 정도, 꽃의 색깔, 식생변화 및 초지에서의 동물상 등을 조사 분석하여 야생화초지의 개발에 필요한 초종 선발 및 구성, 혼파조합 등의 기초자료를 얻고자 실시하였다. 시험은 충남대학교 농과대학 초지 시험포장에서 1997년 8월부터 2000년 12월까지 수행하였으며 시험결과는 다음과 같다. 1) 야생화의 개화는 5월에서 9월까지 연속적으로 유지되었으며 꽃색깔은 비교적 다양하게 유지 되었다. 국내산 야생화의 경우 개화기간이 가장 긴 것은 벌노랑(Lotus corniculatus var. Japonicus)이었고, 원추리(Hemerocallis fulva), 범부채(Belamcanda chinenis), 벌개미취(Aster koraiensis) 등은 발아 및 정착이 느려 문제가 있었다. 외국산 야생화의 경우 국내산 야생화에 비해 초기생육 및 정착이 양호했으며, 꽃색깔도 화려하게 유지되었으나 기생초(Coreopsis tinctoria), 서양톱풀(Achillea millefolium), 원추천인국(Rudbeckia bicolor) 등은 초고가 크거나 강력한 지하경으로 우정되는 경향이었다. 2) 야생화 초지(NWP, IWP)의 적합한 turf grasses 는 계절별로 고른 식생을 유지한 tall fescue, perennial ryegrass, Kentucky bluegrass라 생각된다. 3) 국산 야생화초지의 식생분포는 톱풀(Achillea sibirica) > 벌노랑이(Lotus corniculatus var. Japonicus) > 패랭이꽃(Dianthus chinensis) > 질경이 (Plantago a asiatica) > 민들레(Taraxacum platycarpum) > 제비꽃(Viola mandshurica) > 벌개미취(Aster koraiensis) > 얼치기완두(Vicia tetasperma) > 매듭풀(Lespedeza s st$\psi$ulacea) > 원추리(Hemerocallis fulva) 순이었다. 봄철과 여름철에는 톱풀이, 가을철에는 벌노랑이가 가장 높은 분포를 나타냈으며 계절별로 일정한 분포를 유지한 것은 민들레였다. 한편, 외국산 야생화초지는 서양톱풀(Achillea millefolium) > 기생초(Coreopsis tinctoria) > 끈끈이대나물(Silene armeria) > 금계국(Coreopsis lanceolata) > 원추천인국(Rudbeckia bicolor) > 서양오이풀(Sanguisorba officinalis) > 수레국화(Centaurea cyanus) > 데이지( (Chrysanthemum leucanthemum) > 서양패랭이꽃(Dianthus petraeus) 순 이었다. 봄철에는 끈끈이대나물이, 여름철에는 서양톱풀이 가을철에는 기생초가 가장 높은 식생분포를 나타내었다. 3년 평균 총 야생화의 식생분포는 외국산 야생화초지가 국 산 야생화초지에 비해 2.27배 정도 높은 분포를 보여 다양한 국산야생화 초종도입과 선발 및 순도 높은 종자의 확보가 국산 야생화초지를 보급을 위해 우선적으로 선결해야할 과제라 하겠다. 4) 야생화 초지(NWP, IWP)는 관행 혼파초지나 하번초 혼파초지에 비하여 동물상이 다양하고 많게 분포되었으며 그중 외국산 야생화초지의 동물 개체수가 가장 많게 나타났다. 이상의 결과를 종합할 때, 야생화 초지는 봄부터 가을까지 야생화가 지속되었고, 양서류 및 곤충의 개체 수가 증가되었던 것으로 보아 야생화 초지의 공익적인 측면에서의 활용 가능성도 클 것으로 기대된다고 하겠다. 그러나 국산 야생화초지를 널리 보급하기 위해서는 무엇보다도 자생 야생화의 생육특성을 고려하여 다양하게 선발해야하고 재배증식을 통한 순도 높은 총자의 확보와 보급이 선행되어야 할 것으로 사료된다.

• 헤리티지:역사와 과학
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• 제51권1호
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• pp.48-65
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• 2018

1874년 박규현이 기술한 '파산서당기'를 중심으로 분석한 파산서당의 영건과정과 조경식물의 변화상을 추적한 연구결과는 다음과 같다. 첫째, 대구 달성의 하빈면 묘리 파회에 자리한 살림집 삼가헌과 별당(파산서당)은 사육신 박팽년(1417~1456)의 11대손 박성수로부터 14대손 박규현에 이르기 까지 약 90년간(1783~1874)에 걸쳐 완성되었는데, 풍수적 회룡고미형(回龍顧尾形)으로 회자되는 길처이다. 둘째, 파회마을은 박성수가 1769년(영조 45)에 벗들과 교우하기 위해 초가를 짓고 자신의 호(삼가헌(三可軒))를 당호로 삼은 것으로부터 유래하는데, 둘째아들 박광석이 분가(1783)한 후 사랑채(1826)와 안채(1869)를 지었고, 박광석의 손자 박규현이 1874년에 연을 심어 가꾼 연못과 정자(하엽정)를 추가했으며, 파산서당 명칭은 삼가헌 뒷동산 지명과 연계된다. 셋째, 네모꼴 연못은 길이 21m, 너비 15m 규모로 연꽃을 심었는데, 못 가운데 원형 섬을 두어 조선시대의 보편적인 성리학적 세계관을 반영하고 있다. 파산서당에 부가된 정자(하엽정)의 명칭, 동쪽 방 이연헌(怡燕軒), 서쪽 방 몽양재(蒙養齋) 등은 군자적 삶의 염원, 그리고 자손들의 밝은 미래를 염원하는 뜻을 담았다. 넷째, 성리학적 규범(소나무, 연꽃, 대나무), 안빈낙도의 생활철학(버드나무), 은일사상과 태평성대의 염원(대나무), 그리고 구휼목적과 실용성(밤나무, 상수리나무, 가래나무, 옻나무 등)에 비중을 둔 식재수종의 반영을 추적할 수 있는데, 이는 서당이라고 하는 장소성과 상징성, 절제미학 등을 고려한 조경법이라 하겠다. 다섯째, 조영초기 식재수종은 회화나무와 연꽃을 제외하고 대부분 고사하여 다른 수종으로 대체되었는데, 서당 기능의 상실과 풍수적 가치(홍동백서 상징수종), 벽사기능(엄나무와 탱자나무), 심미성 및 실용적 가치(해당화, 매화, 배나무, 복숭아나무, 굴참나무), 가문의 번영과 변화지 않는 청백의 삶(자귀나무, 배롱나무, 주목), 심미관 등 후손들의 확장된 작정관을 보여주는 사례이다.