• 한국균학회지
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• 제46권3호
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• pp.307-314
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• 2018

본 연구는 알코올 발효능이 우수한 야생 효모를 막걸리 발효에 응용할 목적으로 충남 예산군 예당저수지 야생화에서 분리한 비병원성 야생 효모들 중 에탄올 생산 우수 효모로 선발한 Aureobasidium pullulans P-1의 균학적 특성과 막걸리 발효 조건을 조사하였다. A. pullulans P-1는 출아에 의해 영양증식을 하였고 자낭포자를 생성하는 유포자 효모로서 내당성과 에탄올 내성이 강한 호염성 야생 효모이었다. A. pullulans P-1의 yeast extract-peptone-dextrose 배양액을 주모로 증자미와 입국과 물이 혼합된 술밑에 5% 첨가한 후 $25^{\circ}C$에서 1~10일간 발효시키면서 발효 중의 이화학적 성질의 변화를 조사한 결과, 에탄올 함량은 $25^{\circ}C$로 10일간 발효시켰을 때 가장 많은 8.45%를 생성하였고 관능 특성이 우수하였으며 항고혈압 활성 안지오텐신 전환효소 저해활성이 71.1%로 높았다.

• 원예과학기술지
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• 제31권4호
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• pp.467-472
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• 2013

국내 전역에서 수집된 야생 참외 계통 중 1986년에 20계통, 1990년에 16계통에 대해 재배시험결과는 계통간에 초장에 변이가 있었고 잎은 작은 편이었다. 성표현형은 양성웅화동주형 30계통, 자웅동주형 4계통, 양성화주형이 2계통이었다. 주지에서 암꽃착생은 양성화주형을 제외하고는 극히 드물었다. 양성웅화동주형과 자웅동주형은 측지의 제 1, 2절에는 암꽃착생이 현저하였다. 유과에는 모두 고미질이 확인되었다. 숙과의 색은 진황, 황, 연황, 황녹, 유백색이였으며 과중은 매우 작았고 숙과의 감미는 매우 낮았다. 일과당 종자수는 50.6-158.4개로 야생종 및 종족유지의 특징을 보여주고 있었다.

• 한국환경농학회지
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• 제34권2호
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• pp.149-154
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• 2015

효모의 유용 기능을 탐색하기 위해서 참나리에 정착하는 효모 군집을 분석하였다. 본 연구에서는 잎에서 총 82 균주, 줄기에서 총 94 균주, 꽃에서는 총 97 균주를 분리하였다. 분리된 균주를 ITS 1과 4 primer를 사용하여 ITS 영역 염기서열의 계통분석을 실시한 결과, 참나리 잎에서는 Pseudozyma가 31 균주, Aureobasidium pullulans가 28 균주, Cryptococcus가 11 균주, 줄기에서는 A. pullulans가 40 균주, Cryptococcus가 23균주, Candida 11 균주, 꽃에서는 A. pullulans가 95 균주, Rhodotorula 1 균주, Metschnikowia 1 균주가 분포하였다. 특히, 참나리 잎과 줄기, 꽃 모든 시료에서 A. pullulans가 우점하였으며, 꽃에서는 97 균주 중에서 95 균주의 A. pullulans가 검출되어 한 종이 절대적으로 우점함을 알 수가 있었다. 참나리 잎에서는 82 균주 중에서 Pseudozyma가 31 균주로 가장 우점함을 보였으며, 참나리 줄기에서는 94 분리 균주 중에서 Cryptococcus가 23 균주로 두번째로 우점함을 보였다. 참나리의 부위별로 분포양상이 다름을 확인하였다. 향후 이들 효모 균주들의 바이오테크놀로지 분야에 응용을 기대해본다.

• 한국의류학회지
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• 제28권11호
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• pp.1353-1360
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• 2004

The purpose of this study is to develop a cultural products design of korean image using fabrics. The study is carry out in literature research methods and make sample product. We examine existing researches about cultural product design development to understand developed items, materials, and design directions. The brainstorming method and the established literature researches were used to obtain the design components of Korean image. A cultural products design was developed into the decorative structural details. The results can be summarized as follows: Korean image is pure, passive, gentle, small, and lasting life-force at weeds and wild flowers. Korean form is rounded curve and soft silhouette as streamy, not exaggerate. Korean color is not a finishing treatment, non-dyed natural color, or pastel, light greyish, and dull tone. Korean textile is a natural materials as linen, ramie, and coarse silk. Final sample products are table supplies, home goods and bed goods applying the sewing method of tuck, quilting, top-stitching, patch work, applique and embroidery.

• 생명과학회지
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• 제11권3호
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• pp.242-247
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• 2001

Botrytis cinerea E12 was isolated from the leaves, flowers and fruits of eggplant in the greenhouse in Halrim, Kimhae and Dejeo, Pusan. The leaves infected with the pathogen were appeared initially brown-color, small gray spots at the edge, and finally fall down. The fruit was showed the symptoms of circular or irregular shapes, followed by sunken. When the symptoms were developed, the conidia formed on the surface with gray color. To determine the pathogenicity of B. cinerea E12 against the eggplants, the conidia were suspended with 30% tomato juice, PDB and sterile water, respectively. The result showed that the conidial suspension with 30% tomato juice was highly effective on the pathogenicity as more than 90%. Moreover, the symptoms caused by inoculum were the same as those of wild-type pathogen.

• 농촌지도와개발
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• 제10권2호
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• pp.251-266
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• 2003

Diffusion of non-professional flower growing in Korea still lags behind. The objectives of the study are to establish a theoretical modes to explain the diffusion of social floriculture, to identify some characteristics and problems of growing flower crops to cultivate amenities and emotions, and to suggest remedial measures for fostering it. Theoretical model adopted in this study consists of such variables as education, social movement, policies, diffusion of social flower growing, amenities, and emotional cultivation. For identifying the diffusion characteristics of flower growing, projects implemented by a non-governmental organization, schools, local and central government organizations were analyzed. In order to stimulate the diffusion of social flower growing and to increase its effects, there needs to strengthen education of stakeholders, establishment of partnership among schools, non-governmental and governmental organizations, and institutional supports including manpower and finances. Linking flower growing projects to community festival and tourism development programs can foster institutionalization of community flower growing. Furthermore, the introduction of wild, symbolic and traditional flowers and trees will help to specialize and improve community landscapes.

• 농업과학연구
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• 제46권2호
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• pp.335-340
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• 2019

Two species of Leycesteria, L. gracilis (Kurz) Airy Shaw and L. formosa Wall. (Caprifoliaceae), from the Lao Cai and Yen Bai Provinces are reported here as a new generic record for the flora of Vietnam. During floristic surveys of northern Vietnam, L. gracilis and L. Formosa were encountered. A critical morphological study of all the collected specimens and the type of materials consulting the relevant literature led us to confirm the identity of our collected specimens as L. gracilis and L. formosa. The genus differs from other Vietnamese genera of Caprifoliaceae by the presence of a spike inflorescence or flowers in sessile whorls of 6. Taxonomic notes, description, and photographs are provided, together with short notes on the distribution, ecology and phenology of the two species.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.49-55
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• 2003

The morphology of the leaves and the flowers of angiosperms exhibit remarkable diversity. One of the factors showing the greatest variability of leaf organs is the leaf index, namely, the ratio of leaf length to leaf width. In some cases, different varieties of a single species or closely related species can be distinguished by differences in leaf index. To some extent, the leaf index reflects the morphological adaptation of leaves to a particular environment. In addition, the growth of leaf organs is dependent on the extent of the expansion of leaf cells and on cell proliferation in the cellular level. The rates of the division and enlargement of leaf cells at each stage contribute to the final shape of the leaf, and play important roles throughout leaf development. Thus, the control of leaf shape is related to the control of the shape of cells and the size of cells within the leaf. The shape of flower also reflects the shape of leaf, since floral organs are thought to be a derivative of leaf organs. No good tools have been available for studies of the mechanisms that underlie such biodiversity. However, we have recently obtained some information about molecular mechanisms of leaf morphogenesis as a result of studies of leaves of the model plant, Arabidopsis thaliana. For example, the ANGUSTIFOLIA (AN) gene, a homolog of animal CtBP genes, controls leaf width. AN appears to regulate the polar elongation of leaf cells via control of the arrangement of cortical microtubules. By contrast, the ROTUNDIFOLIA3 (ROT3) gene controls leaf length via the biosynthesis of steroid(s). We provide here an overview of the biodiversity exhibited by the leaf index of angiosperms. Taken together, we can discuss on the possibility of the control of the shapes and size of plant organs by transgenic approaches with the results from basic researches. For example, transgenic plants that overexpressed a wild-type ROT3 gene had longer leaves than parent plants, without any changes in leaf width. Thus, The genes for leaf growth and development, such as ROT3 gene, should be useful tools for the biodesign of plant organs.

• Journal of Species Research
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• 제4권2호
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• pp.152-158
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• 2015

Dendranthema boreale and D. indicum are easily distinguished from other Korean Dendranthema spp. by having yellow flowers. We have found a putative new taxon of Dendranthema having white flowers, except for sharing most characters with Dendranthema boreale. The chloroplast (cp) genome of the putative new taxon of Dendranthema, Dendranthema sp. K247003, registered in National Agro-Biodiversity Center (ABC), was completely characterized as a genetic barcode. The cp-genome of Dendranthema sp. K247003 was 151,175-bp in size: LSC was 82,886-bp, IR 24,971-bp, SSC 18,347-bp. The cp-genome of Dendranthema sp. K247003 contains 113 genes and 21 introns consisted of 79 protein coding genes, 4 RNA genes, and 30 tRNA genes, with 20 group II introns and one group I intron. Some of the genes and there introns were duplicated in IR. The cp-DNA of Dendranthema sp. K247003 is distinguished from that of D. boreale IT121002 by 67 SNPs in genic regions of 24 protein coding genes and by a 9-bp INDEL in ycf1. Further cp-DNA study will give us better information on genetic markers of Dendranthema species.

• Journal of Plant Biotechnology
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• 제38권1호
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• pp.54-61
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• 2011

에틸렌 생합성 효소인 ACC oxidase 유전자인 CAO cDNA가 antisense 방향으로 도입된 형질전환 담배 식물체는 endogenous한 NtACO의 발현 및 에틸렌 생합성이 모두 억제되었다. 또한 폴리아민 생합성 효소인 SAMDC의 유전자 발현 및 효소 활성이 증가하였고 spermidine 함량이 증가하였다. 이러한 결과는 에틸렌 생합성의 감소로 인하여 세포 내 폴리아민 함량의 변화가 유도되는 경쟁적 관계로 spermidine이 증가하였고 이것은 SAMDC의 발현 및 활성 증가를 유도하였다고 여겨진다. 또한 이들 형질전환 식물체는 담배 역병균에 대한 저항 효과 뿐만 아니라 PR 단백질의 유전자들의 발현양이 증가하였는데 이는 에틸렌 감소에 유도되는 폴리아민 중에서도 spermidine의 증가가 PR 단백질의 유전자들의 발현을 촉진시키고 그 결과 병원균 감염에 대한 저항성을 유도하는 기작이 작동한 것으로 여겨진다. Spermidine을 합성하는데 중요하게 작용하는 SAMDC의 활성을 억제하는 MGBG를 처리한 경우에는 PR1a 발현이 억제된 경우에서 이 기작의 가능성을 확인하였다. 결론적으로 병원균 감염에 대한 과정에서 spermidine의 생합성이 증가하게 되면 PR 단백질 등 스트레스 저항성 관련 단백질들이 유도되어 스트레스 저항성을 높일 수 있을 것으로 사료된다.