• Journal of Preventive Medicine and Public Health
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• v.21 no.1 s.23
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• pp.195-206
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• 1988

Two major issues of the blood bank management are quality assurance and inventory control. Recently, in Korea blood donation has gained popularity increasingly to allow considerable improvement of the quality assurance with respect to blood collection, transportation, storage, component preparation skills and hematological tests. Nevertheless the inventory control, the other issue of blood bank management, has been neglected so far. For the supply of blood by donation barely meets the demand, the blood bank policy on the inventory control has been 'the more the better.' The shortage itself by no means unnecessitate inventory control. In fact, in spite of shortage, no small amount of blood is outdated. The efficient blood inventory control makes it possible to economize the blood usage in the practice of state-of-the-art medical care. For the efficient blood inventory control in Korean hospitals, this tudy is to develop formulae forecasting the standard blood inventory level and suggest a set of policies improving the blood inventory control. For this study informations of $A^+$ whole bloods and packed cells inventory control were collected from a University Hospital and the Central Blood Bank of the Korean Red Cross. Using this informations, 1,461 daily blood inventory records were formulated.48 varieties of blood inventory control environment were identified on the basis of selected combinations of 4 inventory control variables-crossmatch, transfusion, inhospital donation and age of bloods from external supply. In order to decide the optimal blood inventory level for each environment, simulation models were designed to calculate the measures of performance of each environment. After the decision of 48 optimal blood inventory levels, stepwise multiple regression analysis was started where the independent variables were 4 inventory control variables and the dependent variable was optimal inventory level of each environment. Finally the standard blood inventory level decision rule was developed using the backward elimination procedure to select the best regression equation. And the effective alternatives of the issuing policy and crossmatch release period were suggested according to the measures of performance under the condition of the standard blood inventory level. The results of this study' were as follows ; 1. The formulae to calculate the standard blood inventory level($S^*$)was $S^*=2.8617X(d)^{0.9342}$ where d is the mean daily crossmatch(demand) for a blood type. 2. The measures of performace - outdate rate, average period of storage, mean age of transfused bloods, and mean daily available inventory level - were improved after maintenance of the standard inventory level in comparison with the present system. 3. Issuing policy of First In-First Out(FIFO) decreased the outdate rate, while Last In-First Out(LIFO) decreased the mean age of transfused bloods. The decrease of the crossmatch release period reduced the outdate rate and the mean age of transfused bloods.

• Applied Biological Chemistry
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• v.50 no.4
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• pp.352-357
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• 2007

For a correlation to study the five tastes and efficacies in oriental medicine concepts, we used herbal medicines with bitter taste character in traditionally effective and experimental methods. In this study, we tested the in vitro effect of 20 typical kinds of herbal medicines with bitter taste on platelet aggregation induced by collagen in human whole blood using the impedance method of aggregometry. Among them, 3 kinds of hot water extracts and 2 kinds of 70% ethanol extracts showed the significant inhibiting effect on whole blood aggregation $(^{***}p<0.001)$. In particular, Coptidis Rhizoma (Coptis chinensis) extracts were selected as the most effective candidate with a strong grade of bitter taste through the sensory test of trained panels. The results from this experiment provide pharmacological evidence for the traditional use of bitter tasting herbs with traditional medicine theory, suggesting that strong bitter taste herbs could be help problems of blood circulation more than mild bitter tasting herbs.

• Journal of Veterinary Clinics
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• v.14 no.1
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• pp.78-87
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• 1997

The study of present study was to determine the valuable laboratory tests for the differential diagnosis of lead poisoning in dogs. Sixteen mongrel dogs were divided into 2 experimental groups (A and B) and a control group (C). The A and B groups were administered orally 2 mg and 20 mg of lead per kilogram of body weight for 49 days, respectively. In addition to clinical observation, blood, urine and hair samples were collected on appointed day and examined for hematological changes, lead content of serum, whole blood and hair, and urinary $\delta$-aminolevulinic acid concentrations. All dogs were necropside on 49th day and examined for the lead content and histological changes of organs. The results obtained were summarized as follows: The group B showed digestive and nervous signs, and weight loss. The group A showed no significant hematological changes except polychromatophilla on the 7th day. But group B showed polychromatophilia as well as mild anemia and nucleated erythrocyte on the 7th and 35th day. Basophlic stippling erythrocytes were observed in some of the group B on the 14th day. The lead content of whole blood was increased significantly in both A and B groups on the 21the day. The urinary $\delta$-aminolevulinic acid content was increased in both A and B groups on the 7th day. The hair lead content of A and B groups was increased significantly on the 49th and 21th day, respectively. The lead contents of organs including liver, kidney, spleen, muscle and bone were increased significantly in group B. Histopathologic changes were characterized by hemorrhages, necrosis and intranuclear inclusion body in the epithelial cells of convoluted tubles of kidney, cloudy swelling and degeneration and/or necrosis of liver, enlargement of Virchow-Robin space, and swelling of endothelial cells and hyperplasia of the pericytes of brain. From these results it may be concluded that examination of nucleated erythrocyte/polychromatophilia, urinary $\delta$ -aminolevulinic acid, and whole blood and hair lead contents is a reliable clinico-pathological diagnostic methods, and that examination of the Virchow-Robin space, endothelial cells and pericytes of brain as well as intranuclear inclusion body in the epithelial cells of convoluted tubles of kidney is valualble postmortem diagnostic methods for lead poisoning in dogs.

• IMMUNE NETWORK
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• v.16 no.5
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• pp.296-304
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• 2016

It has been reported that fatty acid binding proteins (FABPs) do not act only as intracellular mediators of lipid responses but also have extracellular functions. This study aimed to investigate whether extracellular liver type (L)-FABP has a biological activity and to determined serum L-FABP levels in patients with end-stage renal disease (ESRD). We isolated L-FABP complementary deoxyribonucleic acid (cDNA) from the Huh7 human hepatocarcinoma cell line and expressed the recombinant L-FABP protein in Escherichia coli. A549 lung carcinoma and THP-1 monocytic cells were stimulated with the human recombinant L-FABP. Human whole blood cells were also treated with the human recombinant L-FABP or interleukin (IL)-1α. IL-6 levels were measured in cell culture supernatants using IL-6 enzyme-linked immunosorbent assay (ELISA). Human recombinant L-FABP induced IL-6 in a dose-dependent manner in A549, THP-1 cells, and whole blood cells. The blood samples of healthy volunteers and patients with ESRD were taken after an overnight fast. The serum levels of L-FABP in healthy volunteers and ESRD patients were quantified with L-FABP ELISA. The values of L-FABP in patients with ESRD were significantly lower than those in the control group. Our results demonstrated the biological activity of L-FABP in human cells suggesting L-FABP can be a mediator of inflammation.

• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.335-341
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• 2009

Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.

• Journal of Environmental Health Sciences
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• v.35 no.2
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• pp.86-94
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• 2009

This study analyzed the concentration of the lead in blood (n=774) from May, 2007 to Oct 2007. The subject was residents in G, Y, H industrial area, Jeollanam-do, in which exposure to the lead is expected due to the adjacency of the industrial complex. As to the lead concentration in the blood of the residents in the whole exposed region and the comparing region, $2.81{\mu}g/dl$ in the exposed region group, and $2.86{\mu}g/dl$ in the comparing region group Respectively, which indicates that the concentration of the comparing group was higher than that of the exposed group. The geometric mean concentration of lead in blood was $3.26{\mu}g/dl$ as to men, and $2.46{\mu}g/dl$ as to women, which indicates that the concentration of men is higher than that of women (p<0.01). The lead concentration for each age group increased in proportion to age except those under 10 for some substances (p<0.01). As to geometric mean concentration in blood according to the smoking history of the subject, the concentration of the smoking group and the non-smoking group was $3.57{\mu}g/dl$ and $2.66{\mu}g/dl$ respectively, which indicates the former is higher than the latter (p<0.01). To clarify the factors affecting the heavy metal concentration in blood among the subjects, the multiple regression analysis was conducted. As a result, it turned out that as to lead content in blood, gender, age, smoking all affect the lead concentration of the subjects ($R^2$=23.3%).

• The Journal of Korean Medicine
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• v.15 no.2 s.28
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• pp.212-232
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• 1994

I selected the theory of blood stasis and wetness-phlegm among the major 4 causes of Joong Poong(C.V.A.)(wind. fire. deficiency of vital energy and wetness-phlegm) and recent etiology of blood stasis. An experimental studies were done to investigate the effects of Danggieum (removing blood stasis and promoting blood circulation) and Ijintang(dissipating wetness-phlegm) on thrombosis and elevated blood viscosity. The results were as follows; 1. The number of platelets were significantly increased in only Danggieum group. 2. Related to the amount of fibrinogen. only Danggieum group revealed some increase. but both groups revealed no significance. 3. Related to the prothrombin time. Danggieum and Ijintang groups had significant decrease. but Danggieum group revealed more significance. 4. Related to the degree of concentration of FDP, only Danggieum group had significant decrease. 5. Whole blood viscosity and plasma viscosity in lower shear rates. both groups of Danggieum and Ijintang had significant decrease. 6. Related to the amount of RBC. both groups of Danggieum and Ijintang had significant decrease. 7. Related to the change of hematocrit. only Danggieum group, and the change of hemoglobin. only Ijintang group had significant decrease. 8. Related to the change of body temperature. only Ijintang group had significant raise. 9. Related to the changes of WBC. glucose, albumin, total protein and body weight. Danggieum and Ijintang groups had each increase or decrease. but no significance. According to the above results, Danggieum had more significant effect than Ijintang on the thrombosis. and Ijintang had more significant effect than Danggieum on the elevated blood viscosity. And it is considered that it might be more effective in treating Joong-Poong(C.V.A) to take medicines regarding patient's constitution and symptoms as well as the causes of Joong-Poong(C.V.A)

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.1
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• pp.43-48
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• 2010

This study was designed to evaluate whether the addition of sediment improvements with natural materials to water tanks influenced the hematologic properties and muscle glycogen content of the blood cockle, Tegillarca granosa under laboratory conditions. Following a 10-d matured period of sediment improvements, blood cockles were subjected to 4 groups (0, 100, 300, $800\;g/m^2$) of sediment improvements for 14-d Only one individual died at $800g/m^2$ after 48-h Whole blood, hematocrit value and hemoglobin concentration of blood cockle were changed little by sediment improvements, however, both glucose and glycogen were increased more compared with those of the control groups.

• Journal of Animal Science and Technology
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• v.63 no.3
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• pp.475-490
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• 2021

An experiment was conducted to determine the effects of two levels of rumen undegradable protein (RUP) without or with whole or extruded flaxseed on milk yield, milk component, milk fatty acids (FAs) profile and plasma metabolites in transition ewes. Three weeks before and after lambing, seventy-two Baluchi ewes were used in a completely randomized design with a 3 × 2 factorial arrangement of treatments. The treatments contained 1) no flaxseed + 20% RUP (no flaxseed, low RUP [NFLR]); 2) no flaxseed + 40% RUP (no flaxseed, high RUP [NFHR]); 3) 10% whole flaxseed + 20% RUP (whole flaxseed, low RUP [WFLR]); 4) 10% whole flaxseed + 40% RUP (whole flaxseed, high RUP [WFHR]); 5) 10% extruded flaxseed + 20% RUP (extruded flaxseed, low RUP [EFLR]), and 6) 10% extruded flaxseed + 40% RUP (extruded flaxseed, high RUP [EFHR]). Ewes fed 10% extruded flaxseed exhibited higher (p < 0.001) dry matter intake (DMI) and colostrum yield (p < 0.1) compared to other treatments. Two types of flaxseed and RUP levels had no significant effect on milk yield, but milk fat and protein contents decreased and increased in diets containing 40% RUP, respectively. Ewes fed extruded flaxseed produced milk with lower concentrations of saturated fatty acids (SFA) and higher α-linolenic and linoleic acids and also polyunsaturated fatty acids (PUFA) compared to other groups (p < 0.05). During post-lambing, the ewes fed diets containing flaxseed exhibited higher concentration of serum non-esterified FAs (NEFA) compared to diets without flaxseed (p < 0.01). The concentration of serum β-hydroxybutyric acid (BHBA) decreased in the diets containing flaxseed types at pre-lambing, but increased in diets containing extruded flaxseed at post-lambing (p < 0.01). The serum glucose concentration of ewes (pre and post-lambing) which consumed diets containing extruded flaxseed or 40% RUP increased, but blood urea concentration was elevated following supplementation of diet with whole flaxseed or 40% RUP (p < 0.001). In conclusion, utilization of 10% extruded flaxseed in the diets of transition ewes had positive effects on animal performance with favorable changes in milk FAs profile. However, there is no considerable advantage to supply more than 20% RUP level in the diet of transition dairy sheep.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.2
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• pp.139-148
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• 2012

This study was conducted to investigate effects of feeding seleniferous whole crop barley silage (WCBS) on growth performance and blood characteristics in growing Hanwoo steers. A total of 20 growing Hanwoo steers, initially weighing on average 208.8 kg of body weight, were used and treatments were consisted of 1) controls : 0.1 mg/kg Se, 2) T1 : 0.3 mg/kg Se, 3) T2 : 0.9 mg/kg Se by combining seleniferous and/or non-seleniferous WCBS, and 4) T3 : 0.9 mg/kg Se of inorganic Se treatment. Five steers were allocated to each treatment, and the trial was lasted for 90 days. All experimental diets were included to 30% of combination of seleniferous and/or non-seleniferous WCBS, and in T3 diet, sodium selenite that corresponds to 0.9 mg/kg Se was added to control diets. Also, the diets were isonitrogenous and isocaloric among treatments. Dietary level and type of selenium did not affect feed intakes and daily gain, and blood glucose concentration was significantly (p<0.05) lower for controls than T1 and T2 treatments. Blood total lipid concentration was significantly (p<0.05) decreased with increasing levels of dietary selenium, and also that of T2 and T3 was significantly (p<0.05) lower than controls. LDL-cholesterol concentration was significantly (p<0.05) lower for treatments including dietary selenium than controls, and also blood triglyceride concentration was significantly (p<0.05) lower for T2 than controls. Overall, it was tended that feeding seleniferous WCBS or inorganic Se increased blood IgG concentration, and in the same dietary selenium levels, T2 treatment was higher for IgG than T3 group (p>0.05). Blood selenium concentration was significantly (p<0.05) increased by feeding increasing levels of seleniferous WCBS, but there was no significant difference between controls and T3 group. These results showed that feeding seleniferous WCBS to growing Hanwoo steers was responsible for saccharide and lipid metabolism, and in particular, it reduced their total lipid and blood LDL-cholesterol concentrations. Furthermore, selenium present in seleniferous WCBS rather than inorganic selenium was better available in intestinal absorption, and it might help to enhance immunity in growing and younger stages of Hanwoo steers.