Browse > Article

Comparison of diagnostic methods for detection of Brucella species in dog blood samples  

Kwon, Soon-Oh (College of Veterinary Medicine, Kangwon National University)
Lam, Truong Quang (College of Veterinary Medicine, Kangwon National University)
Her, Moon (National Veterinary Research and Quarantine Service)
Ahn, Dong-Chun (College of Veterinary Medicine, Chonbuk National University)
Park, Sang-Hee (National Institute of Health)
Park, Mi-Yeoun (National Institute of Health)
Lee, Young-Ju (College of Veterinary Medicine, Kyuongpook National University)
Hahn, Tae-Wook (College of Veterinary Medicine, Kangwon National University)
Publication Information
Korean Journal of Veterinary Service / v.32, no.4, 2009 , pp. 335-341 More about this Journal
Abstract
Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.
Keywords
Brucella; Dog; Blood sample; PCR; BCSP31; 16S rRNA; OMP-2;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 김성국, 김영환, 조민희, 이영주, 박청규. 2009. 경북지방 소에서 분리한 Brucella abortus의 생화학적 특성. 한국가축위생학회지 32(2): 139-146   과학기술학회마을
2 Carmichael LE, Zoha SJ, Flores-Castro R. 1984. Problems in the serodiagnosis of canine brucellosis: Dog responses to cell wall and internal antigens of Brucella canis. Dev Biol Stand 56: 371-383   PUBMED
3 Gary A. 1964. Infectious disease of domestic animal 3th. Iowa state university press
4 Herman L, De Ridder H. 1992. Identification of Brucella spp. by using the polymerase chain reaction. Appl Environ Microbiol 58(6): 2099-2101
5 Hoff GL, Nichols JB. 1974. Canine brucellosis in Florida: Serologic survey of pound dogs, animal shelter workers and veterinarians. Am J Epidemiol 100(1): 35-39
6 Keid LB, Soares RM, Vasconcellos SA, Megid J, Salgado VR, Richtzenhain LJ. 2009. Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis. Res Vet Sci 86(1): 22-26   DOI   ScienceOn
7 Kim JW, Lee YJ, Han MY Bae DH, Jung SC, Oh JS, Ha GW, Cho BK. 2007. Evaluation of Immunochromatographic assay for serodiagnosis of Brucella canis. J Vet Med Sci 69(11): 1103-1107   DOI   ScienceOn
8 Lucero NE, Escobar GI, Ayala SM, Jacob N. 2005. Diagnosis of human brucellosis caused by Brucella canis. J Med Microbiol 54(5): 457-461   DOI   ScienceOn
9 Queipo-Ortun$\~{n}$ MI, Morata P, Oc$\n P, Manchado P, Colmenero JD. 1997. Rapid diagnosis of human brucellosis by peripheral blood PCR assay. J Clin Microbiol 35(11): 2927-2930
10 Carmichael LE, Joubert JC. 1987. A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant (M-) organism as antigen. Cornell vet. 77(1): 3-12
11 John FT, James HG, Fredric WS, Jeffrey E. 1988. Hagan and Bruner's microbiology and infectious diseases of domestic animals. Barlough. 8th ed
12 오지연, 박청규. 2001. 대구지역 개의 Brucella canis 감염에 대한 세균학적 및 혈청학적 조사. 대한수의학회지 41(1): 67-71
13 Romero C, Pardo M, Grillo MJ, Diaz R, Blasco JM, Lopez-Goni I. 1995b. Evaluation of PCR and indirect enzyme-linked immunosorbernt assay on milk samples for diagnosis of brucellosis in dairy cattle. J Clin Microbiol 33(12): 3198-3200
14 Leal-Klevezas DS, Martinez-Vazquez IO, Martinez-Soriano JP. 1995. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol 33(1): 3087-3090
15 Bae DW, Lee YJ. 2009. Occurrence of canine brucellosis in Korea and polymorphism of Brucella canis isolates by infrequent restriction site-PCR. Korean J Vet Res 49(2): 105-111
16 Blankenship RM, Sanford JP. 1975. Brucella canis. A cause of undulant fever. Am J Med 59(3): 424-426   DOI   ScienceOn
17 Thomsen VO, Kok-Jensen A, Buser M, Philippi-Shulz S, Burkardt HJ. 1999. Monitoring treatment of patients with pulmonary tuberculosis: Can PCR be applied? J Clin Microbiol 37(11): 3601-3607
18 Alton GG, Jones LM, Piet DE. 1975. Laboratory techniques in brucellosis. 2nd Ed. WHO, Geneva: 11-63
19 Tosi MF, Nelson TJ. 1982. Brucella canis infection in a 17-month-old child successfully treated with moxalactam. J Pediatr 101(5): 725-727   DOI
20 Fekete A, Bantle JA, Halling SM. 1992. Detection of Brucella by polymerase chain reaction in bovine fetal and maternal tissues. J Vet Diagn Invest 4(1): 79-83   DOI   ScienceOn
21 Lopez G, Ayala SM, Escobar GI, Lucero NE. 2005. Use of Brucella canis antigen for detection of ovine serum antibodies against Brucella ovis. Vet Microbiol 105(3-4): 181-187   DOI   ScienceOn
22 Fekete A, Bantle JA, Halling SM, Sanborn MR. 1990. Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. J Appl Bacteriol 69(2): 216-227   DOI
23 Cogswell FB, Bantar CE, Hughes TG, Gu Y, Philipp MT. 1996. DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR. J Clin Microbiol 34(4): 980-982
24 Navarro E, Escribano J, Fernandez JA, Solera J. 2002. Comparison of three different PCR methods for detection of Brucella spp. in human blood samples. FEMS Immunol Med Microbiol 34(2): 147-151   DOI
25 Young EJ. 1997. Especies de Brucella. In: Enfermedades infecciosas. Principios y pr$\ctica. Mandell, Bennett and Dolin, Eds. M?dica panamericana, Buenos Aires 2300-2307
26 Romero C, Gamazo C, Pardo M, Lopez-Goni I. 1995a. Specific detection of Brucella DNA by PCR. J Clin Microbiol 33(3): 615-617
27 Wallach JC, Giambartolomei GH, Baldi PC, Fossati CA. 2004. Human infection with M-strain of Brucella canis. Emerg Infect Dis 10(1): 146-148   DOI   ScienceOn
28 Zerva L, Bourantas K, Mitka S, Kansouzidou A, Legakis NJ. 2001. Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J Clin Microbiol 39(4): 1661-1664   DOI   ScienceOn
29 Mateau-de-Antonio EM, Martin M, Soler M. 1993. Use of indirect enzyme-linked immunosorbent assay with ht saline solution extracts of a variant (M-) strain of Brucella canis for diagnosis of brucellosis in dogs. Am J Vet Res 54(7): 1043-1046
30 박인철, 강병규, 문진산, 박용호, 오기석, 이채용, 정석찬. 1999. 전남지방의 소형견 번식장으로부터 발생한 canine brucellosis. 대한수의학회지 39(6): 1099-1105
31 Baily GC, Kraahn JB, Drasar BS, Stokeer NG. 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95(4): 271-275