• Title/Summary/Keyword: wheat resources

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Purification and Properties of Wheat Fructan Exohydrolase (밀 Frucdtan Exohydrolase의 분리 및 특성)

  • Byeong Ryong, Jeong;Thomas L, Housley
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.456-464
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    • 1996
  • Fructans are the major vegetative storage carbohydrate in wheat(Triticum aestivum L.). The depolymerization of fructans occurs by the sequential removal of terminal fructosyl residues by a specific fructan exohydrolase(FEH). The objective of this study was to isolate and characterize this enzyme in wheat. From stems and sheaths of field-grown wheat(cv. Clark), FEH was purified 356-fold using salt precipitation and a series of chromatographic procedures including size exclusion, anion exchange, and affinity chromatography. FEH had a molecular weight of 63.7 kD and an optima at pH 5.5 and 3$0^{\circ}C$. The $K_{m}$s for $\beta$(2 longrightarrow1) linked oligofructans varied, from 10 to 37mM, with the lowest $K_{m}$ for tetrasaccharide. The $V_{max}$ increased as degree of polymerization (DP) increased. Wheat FEH hydrolyzed only, $\beta$(2 longrightarrow1) linked fructans but not, $\beta$(2 longrightarrow6) linked timothy fructan or sucrose. The role of this FEH in fructan metabolism in wheat is discussed.sed.

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Effect of Vital Wheat Gluten on the Quality Characteristics of the Dough Frozen after 1st Fermentation (활성글루텐이 1차발효 후 냉동한 생지의 품질특성에 미치는 영향)

  • Choi, Doo-Ri;Lee, Jeong-Hoon;Yoon, Yoh-Chang;Lee, Si-Kyung
    • Korean Journal of Food Science and Technology
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    • v.37 no.1
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    • pp.55-60
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    • 2005
  • Frozen dough made by sponge and dough method using sweet dough formula was quickly frozen at $-40^{\circ}C$ and stored for 8 weeks at $-20^{\circ}C$. Effects of vital wheat gluten on number of yeast cells, bread volume, specific loaf volume, hardness, and sensory properties of bread were investigated. Dough added with 4% vital wheat gluten showed higher yeast cell survival rate during freeze storage and larger specific loaf volume than other doughs. Hardness value increased with increasing amount of vital wheat gluten added, whereas, in frozen dough stored more than 4 weeks, dough added with 2% vital wheat gluten showed lower hardness value than others. Bread made with 4% vital wheat gluten showed highest sensory score.

Microbial Community Changes in the Soil of Plastic Film House as Affected by Anaerobic Fermentation of Rice Bran or Wheat Bran (쌀겨와 밀기울의 토양 혐기발효 처리가 시설 재배지 토양의 미생물상에 미치는 영향)

  • Kim, Hong-Lim;Weon, Hang-Yeon;Sohn, Bo-Kyun;Choi, Young-Hah;Kwack, Young-Bum
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.341-347
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    • 2009
  • Soil microbial community has been changed after the treatment of anaerobic fermentation using wheat bran or rice bran was applied to the soil. In the dilution plate technique, the number of anaerobic bacteria and fungi was higher in rice bran-treated soil than in non and wheat bran-treated soil, but of yeast was higher in wheat bran-treated soil than in non and rice bran-treated soil. Specially, the fungi were not detected in the wheat bran-treated soil. Identified by 16S rDNA sequencing, the number of aerobic bacteria was similar in all treatments, the dominant bacteria was the genus Bacillus. In the phospholipid fatty acid (PLFA) technique, both Gram-positive and Gram-negative bacteria change slightly in all treatments for 20 days of fermentation process but, after 20day, increased rapidly in wheat or rice bran-treated soil. In conclusion, the microbial communities structure was dramatically changed after the treatment of wheat or rice bran to soil.

An Easy Seedling Method to Screen Resistance of Powdery Mildew of Barley and Wheat (맥류의 흰가루병 저항성 검정을 위한 실내 유묘 대량검정 방법)

  • Park, Jong-Chul;Park, Chul-Soo;Kim, Mi-Jung;Kim, Yang-Kil;Lee, Mi-Ja;Park, Ki-Hoon;Noh, Tae-Hwan
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.153-158
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    • 2008
  • This study was conducted to develop a easy seedling method to test large amounts of barley and wheat cultivars for resistance of powdery mildew. In addition, we also examined the resistance of genetic resources that have been used in barley and wheat breeding programs in Korea. This seedling test used paper bag with seeds can be completed within three weeks. to-day seedlings were available to inoculation and 8 days was needed for result reading. This method can test at least 180 collections at one time. we can identify the two resistant types by leave symptoms showed non infection and necrotic spots. Among the 79 Korean barley cultivars, only two cultivar, 'Sangrokbori' and 'Dajinbori' were resistant and 'Jejubori' showed moderate resistant. There was no resistant in hulless barley and wheat cultivars. It was same results in comparison of earlier resistant reports in field test. We confirm that this method could using in test of powdery mildew resistance in barley and wheat. Among the 1,401 genetic resources using in Korean breeding program, malting barley has more resistant collections comparing to 796 hulled and hulless barley and 273 wheat germplasms.